TY  - JOUR
T1  - Positive effect of BSA supplemented soybean lecithin based extender  on liquid storage of ram semen at 5C
TT  - BSA ilave edilmiş lesitin bazlı sulandırıcının koç spermasının 5 C sıcaklıkta muhafaza edilmesineolumlu etkisi
AU  - Gökçe, Elif
AU  - Alçay, Selim
AU  - Gül, Zülfiye
PY  - 2017
DA  - December
DO  - 10.1501/Vetfak_0000002815
JF  - Ankara Üniversitesi Veteriner Fakültesi Dergisi
JO  - Ankara Univ Vet Fak Derg
PB  - Ankara University
WT  - DergiPark
SN  - 1300-0861
SP  - 313
EP  - 320
VL  - 64
IS  - 4
LA  - en
AB  - The aim of the study was to evaluate different BSA doses in soybean-lecithin based extenders for liquid storage of ram semen. Pooled semen was divided into four equal volumes and diluted in a single-step dilution method with BSA supplemented (BSA5, BSA7.5, BSA10 groups supplemented 5mg/mL, 7.5mg/mL, 10mg/mL BSA respectively) and BSA-free (control) extenders. Semen samples were assessed for sperm motility, plasma membrane functional integrity using hypo-osmotic swelling test (HOST), damaged acrosome using FITC-Pisum sativum agglutinin (PSA-FITC) at 0, 24, 48 and 72h of storage at 5 °C. Antioxidant activities of groups at 0 and 72h were measured with glutathionine (GSH) concentration, membrane lipid peroxidation status was analyzed using the malondialdehyde (MDA) concentration. Antioxidant capacity of extender also was measured with 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and Dichloro-dihydro-fluorescein diacetate (DCFH-DA) assays. In the study, BSA (5mg/mL) supplementation has beneficial effect on ram sperm parameters at all evaluation times. In addition, the increasing doses of BSA supplementation has a negative effect on sperm parameters especially 48 and 72h of storage
KW  - BSA
KW  - lecithin
KW  - ram semen
KW  - short-term storage
N2  - Bu çalışmada, farklı dozlarda BSA ilave edilmiş lesitin bazlı sulandırıcının koç spermasının 5°C’ta kısa sureli saklanması üzerindeki etkisinin değerlendirilmesi amaçlandı. Çalışma amacıyla alınan spermalar pooling yapılarak dört eşit hacme bölündü ve 5mg/mL, 7.5mg/mL, 10mg/mL BSA ilave edilmiş (BSA5, BSA7.5, BSA10 sırasıyla) ve edilmemiş (kontrol) sulandırıcılarla sulandırıldı. Sulandırılmış spermanın sıcaklığı 5°C’a düştükten sonra 0, 24, 48 ve 72. saatlerde; motilite, plazma membran fonksiyonel bütünük [hipoozmotik şişme testi (HOST)] ve akrozomal hasar [FITC-Pisum sativum agglutinin (PSA-FITC)] muayeneleri yapıldı. Çalışmanın başlangıcı ve sonunda antioksidan aktivitenin ve lipid peroksidasyonun değerlendirilmesi için malondialdehyde (MDA) ve glutathionine (GSH) konsantrasyonları ölçüldü. Ayrıca 2,2-Diphenyl-1-picrylhydrazyl (DPPH) ve Dichloro-dihydro-fluorescein diacetate (DCFH-DA) yöntemleri ile her bir sulandırıcının antioksidan kapasiteleri değerlendirildi. Yapılan muayenelerin sonucunda; 5mg/mL BSA ilavesinin koç spermasının kısa süreli muhafazasında etkili olduğu gözlemlendi. Ayrıca artan BSA konsantrasyonunun spermanın soğutularak saklanmasında olumsuz etki oluşturduğu belirlendi
CR  - Agarwal A, Makker K, Sharma R (2008): Clinical relevance of oxidative stress in male factor infertility: An update. J Reprod Immunol, 59, 2-11.
CR  - Akhter S, Rakha BA, Iqbal R, et al. (2014): Effect of bovine serum albumin on motility, plasmalemma, viability and chromatin integrity of buffalo bull spermatozoa and chromatin integrity of buffalo bull spermatozoa. Pakistan J Zool, 46, 115-120.
CR  - Alçay S, Gökçe E, Toker MB, et al. (2016): Freeze-dried egg yolk based extenders containing various antioxidants improve post-thawing quality and incubation resilience of goat spermatozoa. Cryobiology, 72, 269-273.
CR  - Alçay S, Toker MB, Üstüner B, et al. (2014): Investigation of relationships between DNA integrity and fresh semen parameters in rams. Kafkas Univ Vet Fak Derg, 20, 793- 798.
CR  - Alçay S, Toker MB, Gökçe E, et al. (2015): Successful ram semen cryopreservation with lyophilized egg yolk-based extender. Cryobiology, 71, 329-333.
CR  - Amidi F, Farshad A, Khor AK (2010): Effects of cholesterol-loaded cyclodextrin during freezing step of cryopreservation with TCGY extender containing bovine serum albumin on quality of goat spermatozoa. Cryobiology, 61, 94-99.
CR  - Anghel A, Zamfirescu S, Dragomir C, et al. (2010): The effects of antioxidants on the cytological parameters of cryopreserved buck semen. Rom Biotech Lett, 15, 26-32.
CR  - Bakst MR, Cecil HC (1992): Effect of bovine serum albumin on motility and fecundity of turkey spermatozoa before and after storage. J Reprod Fert, 94, 287-293.
CR  - Ball BA, Medina V, Gravance CG, et al. (2001): Effect of antioxidants on preservation of motility, viability and acrosomal integrity of equine spermatozoa during storage at 5C. Theriogenology, 56, 577-589.
CR  - Bouayed J, Bohn T (2010): Exogenous antioxidants- double-edged swords in cellular redox state health beneficial effects at physiologic doses versus deleterious effects at high doses. Oxid Med Cell Longev, 3, 228-237.
CR  - Bucak MN, Çoyan K, Öztürk C, et al. (2012): Methionine supplementation improves ram sperm parameters during liquid storage at 5 C. Cryobiology, 65, 335-337.
CR  - Bucak MN, Tekin N (2007): Protective effect of taurine, glutathione and trehalose on the liquid storage of ram semen. Small Rum Res, 73, 103-108.
CR  - Bucak MN, Tuncer PB, Sarıözkan S, et al. (2009): Comparison of the effects of glutamine and an amino acid solution on post-thawed ram sperm parameters, lipid peroxidation and anti-oxidant activities. Small Rum Res, 81, 13-17.
CR  - Çoyan K, Başpınar N, Bucak MN, et al. (2010): Influence of methionine and dithioerythritol on sperm motility, lipid peroxidation and antioxidant capacities during liquid storage of ram semen. Res Vet Sci, 89, 426-431.
CR  - Davis BK, Byrne R, Hun B (1979): Studies on the mechanism of capacitation. II. Evidence for lipid transfer between plasma membrane of rat sperm and serum albumin during capacitation in vitro. Biochim Biophys Acta, 558, 257-266.
CR  - De Paz P, Esteso MC, Alvarez M, et al. (2010): Development of extender based on soybean lecithin for its application in liquid ram semen. Theriogenology, 74, 663- 671.
CR  - Demircan C, Gül Z, Büyükuysal RL (2014): High glutamate attenuates S100B and LDH outputs from rat cortical slices enhanced by either oxygen-glucose deprivation or menadione. Neurochem Res, 39, 1232-1244.
CR  - Dorman HJD, Peltoketo A, Hiltunen R, et al. (2003): Characterization of antioxidant properties of de-odourized aqueous extracts from selected Lamiaceae herbs. Food Chem, 83, 255-262.
CR  - El-Kon I (2011): Testing usability of bovine serum albumin (BSA) for preservation of Egyptian Buffalo semen. Am Eurasian J Agric Environ Sci, 11, 495-502.
CR  - Ellman GL (1959): Tissue sulfhydryl groups. Arch Biochem Biophys, 82, 70-77.
CR  - Gaspar J, Rodrigues A, Laires A, et al. (1994): On the mechanisms of genotoxicity and metabolism of quercetin. Mutagenesis, 9, 445-449.
CR  - Gündoğan M (2009): Short term preservation of ram semen with different extenders. Kafkas Univ Vet Fak Derg, 15, 429-435.
CR  - Gündoğan M, Tekerli M, Uçar M, et al. (2003): Effect of diluents on motility of ram sperm during storage at 5 C. Arch Androl, 49, 69-75.
CR  - Gündoğan M, Yeni D, Avdatek F, et al. (2010): Influence of sperm concentration on the motility, morphology, membrane and DNA integrity along with oxidative stress parameters of ram sperm during liquid storage. Anim Reprod Sci, 122, 200-207.
CR  - Hodnick WF, Kung FS, Roettger WJ, et al. (1986): Inhibition of mitochondrial respiration and production of toxic oxygen radicals by flavonoids. A structure-activity study. Biochem Pharmacol, 35, 2345-2357.
CR  - Hossain MS, Hyeong LJ, Miah AG, et al. (2007): Effect of fatty acids bound to bovine serum albumin-V on acrosome reaction and utilization of glucose in boar spermatozoa. Reprod Med Biol, 6, 109-115.
CR  - Kankofer M, Kolm G, Aurich J (2005): Activity of glutathione peroxidase, superoxide dismutase and catalase and lipid peroxidation intensity in stallion semen during storage at 5 C. Theriogenology, 63, 1354-1365.
CR  - Matsuoka T, Imai H, Kohno H, et al. (2006): Effect of bovine serum albumin and trehalose in semen diluents for improvement of frozen-thawed ram semen. J Reprod Dev, 52, 675-683.
CR  - Maxwell WMC, Salamon S (1993): Liquid storage of ram semen: A review. Reprod Fertil Dev, 5, 613-638.
CR  - Metodiewa D, Jaiswal AK, Cenas N, et al. (1999): Quercetin may act as a cytotoxic prooxidant after its metabolic activation to semiquinone and quinoidal product. Free Radic Biol Med, 26,107-116.
CR  - Naijian HR, Kohram H, Zare SA, et al. (2013): Effects of various concentrations of BSA on microscopic and oxidative parameters of Mahabadi goat semen following the freeze-thaw process. Small Rum Res, 113, 371-375.
CR  - Ochiai M, Nagao M, Wakabayashi K, et al. (1984): Superoxide dismutase acts as an enhancing factor for quercetin mutagenesis in rat-liver cytosol by preventing its decomposition. Mutat Res, 129, 19-24.
CR  - Sarıözkan S, Tuncer PB, Bucak MN, et al. (2009): Influence of various antioxidants on microscopic-oxidative stress indicators and fertilizing ability of frozen-thawed bull semen. Acta Vet Brno, 78, 463-469.
CR  - Sarıözkan S, Türk G, Cantürk F, et al. (2013): The effect of bovine serum albumin and fetal calf serum on sperm quality, DNA fragmentation and lipid peroxidation of the liquid stored rabbit semen. Cryobiology, 67, 1-6.
CR  - Toker MB Alçay S, Gökçe E, et al. (2016): Cryopreservation of ram semen with antioxidant supplemented soybean lecithin-based extenders and impacts on incubation resilience. Cryobiology, 72, 205- 209.
CR  - Uysal O, Bucak MN (2007): Effect of oxidized serum albumin, cysteine and lycopene on the quality of frozen thawed ram semen. Acta Vet Brno, 76, 383-390.
CR  - Uysal O, Bucak MN, Yavaş İ, et al. (2007): Effect of various antioxidants on the quality of frozen-thawed bull semen. J Anim Vet Adv, 6, 1362-1366.
CR  - Üstüner B, Alçay S, Nur Z, et al. (2014): Effect of egg yolk and soybean lecithin on tris-based extender in post-thaw ram semen quality and in vitro fertility. Kafkas Univ Vet Fak Derg, 20, 393-398.
CR  - Watjen W, Michels G, Steffan B, et al. (2005): Low concentrations of flavonoids are protective in rat H4IIE cells whereas high concentrations cause DNA damage and apoptosis. J Nutr, 135, 525-531.
CR  - Xia J, Ren D (2009): The BSa-induced Ca(2+) influx during sperm capacitation is CATSPER channel-dependent. Reprod Biol Endocrinol, 7, 119-127.
UR  - https://doi.org/10.1501/Vetfak_0000002815
L1  - https://dergipark.org.tr/en/download/article-file/647297
ER  -