TY - JOUR T1 - Tıbbi Öneme Sahip Hatmiler İçin Etkili Bir Çimlendirme Yöntemi: Tohum Kabuğuna Çentik Atılması TT - The Efficient Germination Method For Marhmallows With Medicinal Importance: The Nicking on Seed Coat AU - Battal, Abdulhamit AU - Görmez, Gül AU - Türker, Musa PY - 2019 DA - September Y2 - 2019 DO - 10.17798/bitlisfen.544221 JF - Bitlis Eren Üniversitesi Fen Bilimleri Dergisi PB - Bitlis Eren University WT - DergiPark SN - 2147-3129 SP - 843 EP - 851 VL - 8 IS - 3 LA - tr AB - Malvaceae familyasının üyelerinden biri olan Alcea cinsi tıbbi öneme sahip bazıtürleri içermektedir. Alcea kurdicaAlef. (Devegülü Hatmi) tonsilite, gastrik ülsere, zatürreye, boşaltım sistemienfeksiyonlarına ve saç kırana karşı kullanılan tıbbi bir bitkidir. Buçalışmanın amacı, devegülü hatmi bitkisi için steril şartlar altında etkili birçimlendirme yönteminin geliştirilmesi ve bu bitkinin doku kültürüçalışmalarında kullanılabilir hale getirilmesidir. Bu amaç için, steril birajan olan sodyum hipokloritin üç farklı konsantrasyonu (%5, %7.5 ve %10), 42°Csıcaklıkta inkübasyonu ve tohum kabuğunun bistüri ile çentiklenmesiuygulamaları test edilmiştir. %5 ve %7.5 sodyum hipoklorit konsantrasyonlarınınsterilizasyon için yeterli olmadığı belirlenirken, %10 sodyum hipokloritkonsantrasyonun ise yeterli olduğu gözlenmiştir. Çimlenme indeksi sonuçlarına göre, kabuğu çentiklenen tohumlarhızlıca çimlenirken, 42°C de inkübasyonun çimlenmeyi etkilemediği gözlenmiştir.Yedinci günde, kotiledon, kotiledon sapı ve hipokotil dokuları doku kültürüiçin kullanılabilir duruma gelmiştir. Sekizinci günde, yapraklar oluşmayabaşlamıştır. Yaprak, yaprak sapı ve sürgün ucu eksplantları ise otuzuncu gündedoku kültüründe kullanılabilecek duruma gelmiştir. Sonuç olarak, bu çalışma iletıbbi öneme sahip devegülü hatmi için etkili bir çimlendirme yöntemigeliştirilmiştir. KW - Çimlenme İndeksi KW - Hatmi KW - Alcea N2 - The genus Alceaone of the member of Malvaceae family includes some species having medicinalimportance. Alcea kurdica Alef.(Devegülü Marshmallow) is a medicinal plant used against tonsils, gastriculcers, pneumonia, excretory system infections and pelade. The aim of thisstudy was to develope an efficient germination method under sterile conditionsand to make the plant suitable for tissue culture studies. For this purpose,three different concentrations (5%, 7.5% and 10%) of the sodium hypochloritewhich is a sterile agent, 42°C incubation and damaging on the seed coat withblade were tested. While 5% and 7.5% of sodium hypochlorite concentrations werenot sufficient for sterilization, 10% of sodium hypochlorite concentration wasobserved to be sufficient. According to the results of the germination index,while the seeds nicked on coat were easily germinated, it was observed thatincubation at 42°C did not affect the germination. 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