TY  - JOUR
TT  - Investigation of Effect of the Citrinin on Mitotic Index in the Human Blood Lymphocytes
AU  - Altuntaş, Hamiyet Dönmez
AU  - Coşgun, Gülcan Dumlupinar
AU  - İmamoğlu, Nalan
AU  - Hamurcu, Zuhal
AU  - Liman, Bilal Cem
AU  - Demirtaş, Halil
PY  - 2006
DA  - June
JF  - Sağlık Bilimleri Dergisi
JO  - JHS
PB  - Erciyes University
WT  - DergiPark
SN  - 1018-3655
SP  - 71
EP  - 78
VL  - 15
IS  - 2
KW  - Sitrinin
KW  - lenfosit
KW  - mitotik indeks
N2  - Mycotoxins are secondary metabolites produced by microfungi that are capable of causing disease and death in humans and other animals. In the present study, the mycotoxin citrinin (CTN) was evaluated for their effects on mitotic index (MI) rates of human peripheral blood lymphocytes. Heparinized peripheral blood samples were obtained from six healthy non-smoking individuals (3 females and 3 males) and cultured for 72 h in the culture medium Nutrient Mixture F-10 in 9 different tubes for each individual. Lymphocyte cultures were treated for the last 48 h with CTN at the following concentrations, 10 mM, 20 mM, 40 mM, 60 mM, 80 mM, 100 mM, and 0.1 mM mitomycin c (positive control) and 0.5% absolute ethanol. To block metaphases, at 70 h of incubation, colchicine was added to cultures to give a final concentration of 0.1 μg/ml, and cultures were harvested cells at 72 h. At 60 mM, 80 mM and 100 mM CTN concentrations, significantly reduced MI values in comparison with negative controls were found (p=0.001). In addition, CTN caused a significant concentration-dependent decrease in MI rates in human lymphocytes. These results make thinking that CTN at the high concentrations is cytotoxic in cultured human lymphocytes
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