TY - JOUR T1 - Investigating the Effect of Testosterone Supplement on Heart and Muscle Damage in Rats Applied with Swimming Exercise AU - Lök, Sefa AU - Kulaksız, Ömer PY - 2019 DA - April Y2 - 2019 DO - 10.15314/tsed.552893 JF - Turkish Journal of Sport and Exercise JO - Turk J Sport Exe PB - Selcuk University WT - DergiPark SN - 2147-5652 SP - 170 EP - 174 VL - 21 IS - 1 LA - en AB - AnabolicAndrogenic Steroids which are a synthetic derivative of testosterone are knownto be used by some sportsmen in order to improve performance. The present studywas conducted to investigate the effect of testosterone supplement on heart andmuscle damage in rats applied with swimming exercise. The study was conductedon 30 day-old 27 (Wistar, male) rats that were supplied from “Selçuk UniversityExperimental Medicine Research and Application Center”. The rats were dividedinto 4 groups. The rats in control groups (n:6) were fed during the studywithout doing any application. The rats in testosteronegroup (n:7) weresupplied with a 10 mg/kg dose of testosterone 5 days a week for 3 weeks. Therats in exercise group (n:7) did freestyle swimming exercise for 30 minutes aday for 5 days a weekfor 3 weeks. The rats in testosterone + exercise group(n:7) did freestyle swimming exercise for 30 minutes and were administered with10 mg/kg dose of testosterone supplement for 5 days a week for 3 weeks. At theend of the trial, necessary biochemical parameters were measured in serumsobtained by drawing blood from tails of the rats. While levels of Serum, LDH,CK-MB, and CKwere studied by using an autoanalyzer, Troponin I level wasanalyzed by using ELISA kit. SPSS 15.0 packaged software was usedin statisticalevaluation of the data and the results were given as mean±SE. Since the datawere convenient with the normal distribution, ANOVA test was applied forbetweengroups comparison and Duncan test was used to identify from which groupthe difference was resulted. When between-groups values of troponin I, ck-MB,and LDH, which are indicators of heart damage, and enzyme CK, which is anindicator of muscle damage,for control, exercise, testosterone, andtestosterone+exercise groups were compared, a statistically significantdifference was determined between control, exercise, testosterone, andtestosterone+exercise groups (p<0.05). As a result of statistically analysisperformed on all groups, the difference was found to be caused by testosteroneand testosterone+exercise groups. Because testosterone production is naturallyincreased in the body during the exercise, swimming exercise and testosteroneadministration led to significant occurrence of troponin I, ck-MB, LDH, whichare heart damage indicators in rats, and CK value, which is an indicator ofmuscle damage, in the blood.Keywords: Testosterone,Heart, Muscle Damage, Swimming Exercise KW - Testosterone KW - Heart KW - Muscle Damage CR - 1. Barkhe MS, Yesalis CE. Abuse of anabolic androgenic steroids and related substances in sport and exercise. Current Opinion in Pharmacology, 2004 :4, 614-20. CR - 2. Borges T, Eisele G, Byrd C, 2001. Rewiev of androgenic anabolic steroid use. Office of Safeguards and Security U.S. Department of Energy, 1-18. CR - 3. Buskens V, Raub W, Van Miltenburg N, Montoya ER, Van Honk J, 2016. Testosterone Administration Moderates Effect of Social Environment on Trust in Women Depending on Second-to-Fourth Digit Ratio. Scientific reports, 6:45-51. CR - 4. Carrier N, Saland SK, Duclot F, He H, Mercer R, Kabbaj M. The anxiolytic andantidepressant like effects of testosterone and estrogen in gonadectomized male rats. Biological psychiatry, 2015; 78(4), 259-69. CR - 5. Casavant MJ, Blake K, Pharmd JG, Yates A, Copley LM. Consequences of use of anabolic androgenic steroids. Pediatr Clin N Am, 2007; 54, 677-90. CR - 6. Comeglio P, Cellai I, Filippi S, Corno C, Corcetto F, Morelli A, Maggi M. Differential Effects of Testosterone and Estradiol on Clitoral Function: An Experimental Study in Rats. The Journal of Sexual Medicine, 2016: 13(12), 1858-71. CR - 7. Fontana K Campos GER, Staron RS, Cruz-Höfling MA, Effects of Anabolik Steroids and High-Intensity Aerobic Exercise on Skeletal Muscle of Transgenic Mice. PLoS One, 2013: 8(11), 1-8. CR - 8. Harmer PA. Anabolic-androgenic steroid use among young male and female athletes: is the game to blame. Br J Sports Med, 2010: 44, 26-31. CR - 9. Hassan AF, Kamal MM. Effect of exercise training and anabolic androgenic steroids on hemodynamics, glycogen content, angiogenesis and apoptosis of cardiac muscle in adult male rats. Int J Health Sci, 2013: 7(1), 47-60. CR - 10. Hoffman JR, Ratamess NA. Medical ıssues associated with anabolic steroid use: are they exaggerated. Journal of Sports Science and Medicine, 2006: 5, 183-93. CR - 11. Kerr JM, Congeni JA . Anabolic-androgenic steroids: use and abuse in pediatric patients. Pediatr Clin N Am, 2007: 54, 7771-85. CR - 12. Kicman AT. Pharmacology of anabolic steroids.British Journal of Pharmacology, 2008: 154, 502-21. CR - 13. Maravelias C, Dona A, Stefanidou M, Spiliopoulou C. Adverse effects of anabolic steroids in athletes: A constant threat. Toxicol Lett, 2005: 158, 167-75. CR - 14. Naraghi MA, Abolhasani F, Kashani I, Anarkooli IJ, Hemadi M, Azami A, Barbarestani M, Aitken RJ, Shokri S. The effects of swimming exercise and supraphysiological doses of nandrolone decanoate on the testis in adult male rats: a transmission electron microscope study. Folia Morphol, 2010: 69 (3), 138–46. CR - 15. Papazisis G, Kouvelas D, Mastrogianni A, Karastergiou A. Anabolic androgenic steroid abuse and mood disorder. A case report Int J Neuropsychopharmacol, 2007: 10(2), 292-293. CR - 16. Rocha FL, Carmo EC, Roque FR, Hashimoto NY, Rossoni LV, Frimm C, Ane'as I, Negra'o CE, Krieger JE, Oliveira EM. Anabolic steroids induce cardiac renin-angiotens in system and impair the beneficial effects of aerobic training in rats. Am J Physiol Heart Circ Physiol, 2007: 293(6), 3575-83. CR - 17. Shokri S, Hemadi M, Bayat G, Bahmanzadeh M, Jafari-Anarkooli I, Mashkani B. Combination of running exercise and high dose of anabolic androgenic steroid, nandrolone decanoate, increasesprotamine deficiency and DNA damage in rat spermatozoa. Andrologia, 2014: 46(2), 184-90. CR - 18. Tasgin E, 2013. The effect of nandrolone decanoate on the concentrations of IL1-β, TNF-α, IL-4, IL-6 and biocheminal parameters in male and female rats. Selcuk University Health Sciences Institute, Phd Thesis, 36-42. CR - 19. Yavari A. Abuse of anabolic androgenic steroids. Journal of Stress Physiology & Biochemistry, 2009: 5(3), 22-32. UR - https://doi.org/10.15314/tsed.552893 L1 - https://dergipark.org.tr/en/download/article-file/713244 ER -