@article{article_559194, title={A protocol on in vitro rooting of ‘Bayrampaşa’ artichoke (Cynara scolymus L.)}, journal={Mediterranean Agricultural Sciences}, volume={32}, pages={129–134}, year={2019}, DOI={10.29136/mediterranean.559194}, author={Ozsan, Tugce and Onus, Ahmet Naci}, keywords={Asteraceae,Cynara scolymus L.,Plant tissue culture,Activated charcoal,Plant growth regulators}, abstract={<p class="MsoNormal" style="margin-bottom:.0001pt;text-align:justify;line-height:200%;"> </p> <p class="MsoNormal" style="margin-top:0cm;margin-right:-3.1pt;margin-bottom:.0001pt;margin-left:-2pt;text-align:justify;"> <span style="font-size:8pt;" lang="en-us" xml:lang="en-us"> <span style="font-size:14px;">Artichoke, belongs to
the family </span> <i> <span style="font-size:14px;">Asteraceae </span> </i> <span style="font-size:14px;"> ( </span> <i> <span style="font-size:14px;">Compositae </span> </i> <span style="font-size:14px;">), is cultivated in a very
wide area in the world and regarded as a functional food due to the bioactive
components. The propagation of artichoke via tissue culture offers considerable
advantages. However one of the most important problem is </span> <i> <span style="font-size:14px;">in vitro </span> </i> <span style="font-size:14px;"> rooting. Therefore, the main purpose of the present study
was to develop an effective protocol on </span> <i> <span style="font-size:14px;">in
vitro </span> </i> <span style="font-size:14px;"> rooting of ‘Bayrampaşa’ cultivar which is one of the important local
artichoke cultivar. To serve the purpose, after a successful micropropagation
process, involving 3 subculture stages, well-developed plantlets were selected
and rooted in different media compositions as ten different media including
control group were used in present study. The differences between the media
were provided by adding different growth regulators such as </span> </span> <span style="font-size:8pt;" lang="en-us" xml:lang="en-us"> <span style="font-size:14px;">10.0 mg l </span> <sup> <span style="font-size:14px;">-1 </span> </sup> <span style="font-size:14px;"> </span> <span style="font-size:14px;">indole-3-acetic
acid (IAA), </span> <span style="font-size:14px;">6.0 mg l </span> <sup> <span style="font-size:14px;">-1 </span> </sup> <span style="font-size:14px;"> </span> <span style="font-size:14px;">indole butyric acid (IBA), </span> <span style="font-size:14px;">5.0 mg l </span> <sup> <span style="font-size:14px;">-1 </span> </sup> <span style="font-size:14px;"> </span> <span> <span style="font-size:14px;">gibberellic acid (GA </span> <sub> <span style="font-size:14px;">3 </span> </sub> <span style="font-size:14px;">), </span> </span> <span style="font-size:14px;">0.5
mg l </span> <sup> <span style="font-size:14px;">-1 </span> </sup> <span style="font-size:14px;"> </span> <span> <span style="font-size:14px;">naphthaleneacetic
acid (NAA) and activated charcoal (0, 1.0 and 2.0 g l </span> <sup> <span style="font-size:14px;">-1 </span> </sup> <span style="font-size:14px;">).
Developments of plantlets were observed and recorded at 15 days intervals.
According to the results obtained during the study, the medium containing IAA
(10.0 mg l </span> <sup> <span style="font-size:14px;">-1 </span> </sup> <span style="font-size:14px;">) and 1.0 g l </span> <sup> <span style="font-size:14px;">-1 </span> </sup> <span style="font-size:14px;"> of activated charcoal gave
the best results in terms of rooting after micropropagation. </span> </span> </span> <span style="font-size:6pt;" lang="en-us" xml:lang="en-us"> <span style="color:#000000;"> </span> </span> </p> <p class="MsoNormal" style="margin-bottom:.0001pt;text-align:justify;line-height:200%;"> <span style="font-size:12pt;line-height:200%;font-family:’Times New Roman’, serif;"> <br /> </span> <span style="font-size:12pt;line-height:200%;font-family:’Times New Roman’, serif;"> </span> </p> <p> </p>}, number={2}, publisher={Akdeniz University}