@article{article_577183, title={Rapid isolation of rosmarinic acid from Ocimum basilicum using flash chromatography}, journal={International Journal of Chemistry and Technology}, volume={3}, pages={72–76}, year={2019}, DOI={10.32571/ijct.577183}, author={Akşit, Hüseyin and Keçeci, Mehmet and Demirtaş, İbrahim and Genç, Nusret}, keywords={Rosmarinic acid,flash chromatography,Ocimum basilicum}, abstract={<p class="MsoNormal" style="text-align:justify;"> <span lang="en-us" style="font-size:9pt;" xml:lang="en-us">In this study; it was aimed at
the isolation of rosmarinic acid (RosA) from <i>Ocimum basilicum </i> (OB) with
a selective extraction and rapid isolation technique using flash chromatography
(FC). For this purpose, dried leaves of OB were extracted with n-hexane,
dichloromethane, and methanol, respectively. The methanol extract (ME) was
extracted with dichloromethane and ethyl acetate using separation funnel by
suspending with water. The ethyl acetate layer was dried with anhydrous Na <sub>2 </sub>SO <sub>4 </sub>
and the solvent was evaporated to give a rosmarinic acid-rich extract. Then the
ethyl acetate extract (EAE) was separated over silica gel column using FC, and
thus RosA was isolated with high purity.  </span> </p> <p> </p>}, number={1}, publisher={Rabia ACEMİOĞLU}