TY - JOUR T1 - Antalya’da yetiştirilen holstein sığırlarında kompleks vertebral malformasyon kalıtsal hastalığının allele özgü PCR ile belirlenmesi TT - Detection of complex vertebral malformation in holstein cattle reared in Antalya using allele-spesific PCR AU - Balcıoğlu, Murat Soner AU - Eren, Murat Gökçe AU - Demir, Eymen PY - 2019 DA - December DO - 10.29136/mediterranean.578015 JF - Mediterranean Agricultural Sciences PB - Akdeniz University WT - DergiPark SN - 2528-9675 SP - 443 EP - 446 VL - 32 IS - 3 LA - tr AB - Bu çalışmada kalıtsal bir hastalık olan kompleks vertebralmalformasyonun (CVM) Antalya ilinde yetiştiriciliği yapılan Siyah Alacasığırlarında varlığı allele özgü polimeraz zincir reaksiyonu (AS-PCR)kullanılarak araştırılmıştır. Antalya Damızlık Sığır Yetiştiricileri Birliğineüye işletmelerden ve aynı birliğe üye olan Akdeniz Üniversitesi, ZiraatFakültesi süt sığırcılığı işletmesine ait toplam 200 bireyde CVM kalıtsalhastalığının varlığı araştırılmıştır. Holstein sığırlarından elde edilen kanörneklerinden total genomik DNA izoleedilmiştir. Sığır genomunun 3. kromozomu üzerindebulunan SLC35A3 geninin 395 bçuzunluğundaki bölgesinde normal ve mutant allellerin belirlenmesinde farklıprimerler kullanılmıştır. AS-PCR işleminden sonra uygulanan agaroz jelelektroforezi sonuçlarına göre bireyler genotiplendirilmiştir. Bu çalışmada;incelenen 200 bireyden 7 tanesinin CVM taşıyıcısı olduğu belirlenmiştir. KW - CVM KW - SLC35A3 geni KW - Kalıtsal hastalıklar N2 - Thisstudy aimed to analyze complex vertebral malformation (CVM) genetic defect inHolstein raised in Antalya province using allele specific PCR (AS-PCR). Thepresence of CVM mutant allele was investigated in 200 Holstein cows raised bymember breeders of Antalya Cattle Breeders Association and raised in AkdenizUniversity, Faculty of Agriculture. Total genomicDNA was extracted from blood samples obtained from Holstein cows. In the 395 bplength of SLC35A3 gene region ofbovine chromosome 3, different primers were used toidentify the normal and mutant allele. By using agarose gel electrophoresis,individuals were genotyped after AS-PCR. In this study, 7 of 200 individualswere found as CVM carriers. CR - Adamov N, Mitrov D, Esmerov I, Dovc P (2014) Detection of recessive mutations (BLAD and CVM) in Holstein-Friesian cattle population in Republic of Macedonia, Macedonian Veterinary Review 37: 61-68. CR - Agerholm JS, Bendixen C, Andersen O, Arnbjerg J (2001) Complex vertebral malformation in Holstein calves, Journal of Veterinary Diagnostic Investigation 16: 283-289. CR - Agerholm JS, Bendixen C, Arnbjerg J, Andersen O (2004) Morphological variation of ‘complex vertebral malformation’ in Holstein calves Journal of Veterinary Diagnostic Investigation 16: 548-553. 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