@article{article_588730, title={Analysis of WRKY Transcription Factors in Barley Cultivars Infected with Fusarium culmorum}, journal={International Journal of Life Sciences and Biotechnology}, volume={2}, pages={165–174}, year={2019}, DOI={10.38001/ijlsb.588730}, author={Uluhan, Ebru and Keleş, Esra Nur and Tufan, Feyza}, keywords={Barley,Fusarium culmorum,Fusarium crown rot,WRKY}, abstract={<span lang="en-us" style="font-size:10pt;line-height:107%;font-family:’Times New Roman’, serif;" xml:lang="en-us">One of the most critical problems of cereal breeding
is <i>Fusarium </i> crown rot disease caused
by various <i>Fusarium </i> species. <i>Fusarium culmorum </i> is one of the
predominant pathogen in Turkey and causes serious product losses. In this
study, the early response of barley cultivars upon <i>F. culmorum </i> infection were analyzed by disease severity and gene
expression patterns of WRKY transcription factors. In that context, firstly,
disease severities of 9 barley cultivars ( <i>Hordeum
vulgare </i> L. cvs. Epona, Escadre, Gazda, Oliver, Avcı 2002, Burakbey, Tarm
92, Manava, and Ramata) infected with <i>F.
culmorum </i>were determined with disease index percentages. After 7 days of
infection, Epona was more sensitive than the other cultivars while the lowest
disease index was seen in Gazda. Total RNA extractions were performed at 72 hai
from the root tissues of Epona and Gazda. Expression analysis of <i>HvWRKY6 </i>, <i>HvWRKY9 </i>, <i>HvWRKY24 </i>, <i>HvWRKY25 </i>, <i>HvWRKY33 </i>, <i>HvWRKY34 </i>, <i>HvWRKY42 </i>, <i> </i>and <i>HvWRKY46 </i>genes were
conducted by qPCR. As a result of pathogen stress, it was observed that the
transcript levels of <i>HvWRKY33 </i> was
significantly upregulated in both cultivars. <i>HvWRKY6 </i>, <i>HvWRKY34 </i> and <i>HvWRKY46 </i> genes were increased in Epona
while upregulation of <i>HvWRKY25 </i> and <i>HvWRKY34 </i> genes were detected in Gazda.
No significant decreases were detected in any cultivars. This study is
important in terms of providing an association between <i>WRKY </i> genes and pathogen stress response. </span>}, number={3}, publisher={International Society of Academicians}, organization={TUBITAK}