TY  - JOUR
T1  - LENTIL SEED ASPARTATE APvIINOTRANSFERASE ISOENZYMES I. ISOLATION and PARTIAL PURIFICATION
TT  - LENTIL SEED ASPARTATE APvIINOTRANSFERASE ISOENZYMES I. ISOLATION and PARTIAL PURIFICATION
AU  - R. Yanardağ**, N. Akev*, A. Can*
PY  - 2015
DA  - September
JF  - Journal of Faculty of Pharmacy of Istanbul University
PB  - Istanbul University
WT  - DergiPark
SN  - 0367-7524
SP  - 1
EP  - 10
VL  - 31
IS  - 0
LA  - en
AB  - Three electrophoretically distinct aspartate minotransferase isoenzymesnamed AAT-1, AAT-2and AAT-3, were separated from lentil (Lens culinarisMedik.) seeds by extraction, ammonium sulphate precipitation, hydroxylapatiteand DEAE-cellulose column chromatographies. AAT-1 was purified 228,AAT-2,42 and AAT-3, 3.8 fold. The isoenzymes were examined by means ofpolyacrylamide gel electrophoresis.Owing to its key role in general metabolism, aspartate adotransferase (AAT, E.C. 2.6.1.1.) which was formerly called glutamic oxaloacetic transaminase (GOT), occurs widely in nature. In animals, cytbsolic and rnitochon+al forms of AAT have been characterized and purified (1-4). AAT has also been studied in various plants (5, 6) and different isoeozyrnic forms have been r&-, ported. The enzyme has been extensively characterized in wheat germ (7,8), cauliflower (9), cotton seeds (lo), pea (ll), soybean (12-14), oat (15), lupine (16) and alfalfa (17,18).
KW  - Lentil
KW  - Lens culinaris Medik.
KW  - aspartate minotransferase
KW  - isoenzymes
N2  - Elektroforetik balurndan far& ii~ aspartat aminotransferaz izoenzimi yegil mercimek (Lens culinaris Medik.) tohudarmdan ekstraksiyon , amonyum sulfatla goktiirme, hidroksilapatit ve DEAE-seluloz sutun kromatografisi yontemleri kullanllarak aynld~. AAT-1, AAT-2 ve AAT-3 olarak adlandlrilan bu izoenzimler, slras~yla 228, 42 ve 3.8 kez saflagtlrdd~. Izoenzimler poliakrilamid jelelektroforezi ile incelendi.Elektroforetik balurndan far& ii~ aspartat aminotransferaz izoenzimi yegil mercimek (Lens culinaris Medik.) tohudarmdan ekstraksiyon , amonyum sulfatla goktiirme, hidroksilapatit ve DEAE-seluloz sutun kromatografisi yontemleri kullanllarak aynld~. AAT-1, AAT-2 ve AAT-3 olarak adlandlrilan bu izoenzimler, slras~yla 228, 42 ve 3.8 kez saflagtlrdd~. Izoenzimler poliakrilamid jelelektroforezi ile incelendi.
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UR  - https://dergipark.org.tr/en/pub/iujfp/article/5921
L1  - https://dergipark.org.tr/en/download/article-file/5225
ER  -