TY  - JOUR
T1  - Determination of Biochemical Profile of Bilecik Propolis
AU  - Keskin, Merve
AU  - Keskin, Şaban
AU  - Mayda, Nazlı
AU  - Özkök, Aslı
PY  - 2019
DA  - December
Y2  - 2019
DO  - 10.15671/hjbc.593940
JF  - Hacettepe Journal of Biology and Chemistry
JO  - HJBC
PB  - Hacettepe University
WT  - DergiPark
SN  - 2687-475X
SP  - 403
EP  - 409
VL  - 47
IS  - 4
LA  - en
AB  - Önemli bir arı ürünü olan propolis antioksidan, antimikrobiyal, antitumoral,antiinflamatuar özellikleri nedeniyle apiterapi uygulamalarında ve günlük yaşamda gıda takviyesi olarakkullanılmaktadır. Propolisin özellikleri elde edildiği bölgeye, toplanmabiçimine ve toplanma zamanına bağlı olarak değiştiğinden her kovandan veyabölgeden aynı özelliklere sahip propolis eldesi mümkün olmamaktadır. Yapılan buçalışmayla Marmara Bölgesinde yer alan Bilecik ilinden temin edilen hampropolis örneklerinin biyokimyasal özellikleri belirlendi. Meşe, kayın, köknar,ıhlamur, kestane ağaçları ile çevrili olan Bilecik orman bakımından oldukçazengindir. Bu nedenle de zengin bir floraya sahiptir. Bu amaçla; %70’lik etilalkol çözeltisi kullanılarak ham propolislerden etanolik propolis özütlerihazırlandı. Hazırlanan propolis ekstraktlarının balsam miktarı, vaks miktarı,toplam polifenol miktarı ve toplam flavonoid miktarı belirlendi. GC/MSkullanılarak ham propolislerin içerdiği bileşenler tespit edildi. Propolis içinönemli bir değer olan balsam miktarının % 13 ile %52 arasında değiştiği; toplampolifenol miktarının ise 11 mg GAE/mL ile 76 mg GAE/mL arasında değiştiğibelirlendi. Propolis örneklerinin aldehitler, alifatik asit ve esterler,alkoller, hidrokarbonlar, karboksilik asit esterleri, ketonlar, terpenler, yağasitleri ve diğer bileşenler bakımından zengin olduğu belirlendi.
KW  - :  Propolis
KW  - phenolic components
KW  - characterization
KW  - balsam
CR  - 1. M. R., Ahn, S., Kumazawa, Y., Usui, J., Nakamura, M.,Matsuka, F. Zhu, and Nakayama, T. Antioxidant Activity and Constituents of Propolis Collected in Various Areas of China, Food Chemistry, 101 (2007),1383-1392.
CR  - 2. R., Aliyazıcioglu, H., Sahin, O., Erturk, E., Ulusoy, ve S., Kolayli, Properties of phenolic composition and biological activity of propolis from Turkey, International Journal of Food Properties, 16 (2013)., 277-287.
CR  - 3. F., Li, S., Awale, Y., Tezuka and S., Kadota, Cytotoxic Constituents From Brazilian Red Propolis and Their Structure–Activity Relationship, Bioorg., Med., Chem., 16 (2008), 5434–5440
CR  - 4. R.O., Silva, V.M., Andrade, E.S., Bulle Rego, G.A., Azevedo Doria, B.,Santos Lima, F.A., Silva, A.A.S., Araújo, Acute and sub-acute oral toxicity of Brazilian red propolis in rats. Journal of Ethnopharmacolgy, 170 (2015), 66-71.
CR  - 5. M.S. R., Neto, S.R., Tintino, A.R.P., da Silva, M.S., Costa, A.A., Boligon, E.F.F., Matias, V.Q., Balbino, R.A., Irwin Menezes and H.D.M. Coutinho, Seasonal variation of Brazilian red propolis: Antibacterial activity, synergistic effect and phytochemical screening. Food and Chemical Toxicology, 107 (2017), 572-580.
CR  - 6. Ö., Saral, Determination of Antioxidant Activities of the Chestnut and Flower Honeys Collected from Eastern Black Sea Region in Turkey. Journal of Apitherapy and Nature, 1 (2018), 28-32.
CR  - 7. E., Talla, A., Tamfu, P., Biyanzi, P., Sakava, F., Asobo, J., Mbafor, N. F., Tchuenguem and R., Ndjouenkeu,. Phytochemical Screening, Antioxidant Activity, Total Polyphenols and Flavonoids Content of Different Extracts of Propolis From Tekel (Ngaoundal, Adamawa Region, Cameroon), The Journal of Phytopharmacology, 3(2014), 5, 321-329.
CR  - 8. M., Popova, E., Giannopoulou, K., Skalicka-Wo´zniak, K., Graikou, J., Widelski, V., Bankova, H., Kalofonos, G., Sivolapenko, K., Gaweł- B˛eben, B., Antosiewicz, and I., Chinou, Characterization and Biological Evaluation of Propolis from Poland, Molecules, 22 (2017), 1159.
CR  - 9. X., Feás, L., Pacheco, A., Iglesias and L.M., Estevinho, Use of Propolis in the Sanitization of Lettuce,, Int. J. Mol. Sci.,15 (2014), 12243-12257.
CR  - 10. V. L. Singleton and J. A., Rossi Colorimetry of Total Phenolics with Phosphomolybdic–Phosphotungstic Acid Reagents, American Journal of Enology and Viticulture, 16 (1965), 144-158. 11. V. L., Singleton, R.Orthofer and R. M., Lamuela-Raventos, Analysis of Total Phenols and Other Oxidation Substrates and Antioxidants by Means of Folin-Ciocalteu Reagent, Methods in Enzymology, 299 (1999), 152-178.12. L. R., Fukumoto and G., Mazza, Assessing antioxidant and prooxidant activities of phenolic compounds, Journal Agriculture Food Chemistry, 48 (2000), 3597-3604. 13. B., Trusheva, M., Popova, E. B., Koendhori, I., Tsvetkova, C., Naydenski, and V., Bankova, Indonesian propolis: Chemical composition, biological activity and botanical origin, Natural Product Research, 25 (2011), 606–613. 14. J.S., Bonvehi, F.V., Coll, and R.E., Jorda, The Composition Active Components and Bacteriostatic Activity of Propolis in Dietetics, JAOCS, 71 (1994), 5, 18-25.
CR  - 15. M., Keskin and S., Kolaylı, Standardization of Propolis, is it Possible?, Uludağ Arıcılık Dergisi – Uludag Bee Journal, 18 (2) (2018): 101-110.16. V., Bankova, S., De Castro and M., Marcucci,  Propolis: recent advances in chemistry and plant origin, Apidologie, Springer Verlag, 31 (1) (2000), 3-15.
CR  - 17. J. S. Bonvehi and A. L.. Gutierrez, Antioxidant Activity and Total Phenolics of Propolis from the Basque Country (Northeastern Spain), Am. Oil Chem. Soc., 88 (2011), 1387–1395.
CR  - 18. L.G., Dias, A.P., Pereira and L. M., Estevinho, Comparative Study of Different Portuguese Samples of Propolis: Pollinic, Sensorial, Physicochemical, Microbiological Characterization and Antibacterial Activity, Food and Chemical Toxicology, 50 (2012), 4246– 4253.
CR  - 19. R. G., Woisky, and A., Salatino,. Analysis of propolis: some parameters and procedures for chemical quality control, Journal of Apicultural Research, 37(2) (1998): 99-105.
CR  - 20. V., Bankova, A., Dyulgerov, S., Popov and N., Marekov, A GC/MS Study of the Propolis Phenolic Constituents, Z. Naturforsch, 42 (1987), 147–151.
CR  - 21. A.O., Sarıkaya, E., Ulusoy., N., Öztürk, M., Tunçel and S., Kolaylı,  Antioxidant Activity And Phenolic Acidconstituents Of Chestnut (Castania Sativamill.) Honey And Propolis Journal of Food Biochemistry, 33 (2009) 470–481
CR  - 22. N., Duran, M., Muz and G., Culha, G., Duran and B., Ozer, GC-MS analysis and antileishmanial activities of two Turkish propolis types, Parasitol Res., 108 (2011):95–105
CR  - 23.  M. Popova, S. Silici, O. Kaftanoglu and V. Bankova, Antibacterial activity of Turkish propolis and its qualitative and quantitative chemical composition, Phytomedicine, 12 (2005), 221–228.
CR  - 24. H., Katircioglu and N., Mercan,  Antimicrobial activity and chemical compositions of Turkish propolis from different regions,  African Journal of Biotechnology, 5 (11) (2006), 1151-1153
CR  - 25. S., Silici and S., Kutluca, Chemical composition and antibacterial activity of propoliscollected by three different races of honeybees in the same region,  Journal of Ethnopharmacology,  99 (2005) 69–73.
CR  - 26. A., Uzel, K., Sorkun, O., Onçağ, D., Çoğulu, O, Gencay and B., Salih, Chemical compositions and antimicrobial activitiesof four different Anatolian propolis samples, Microbiological Research, 160(2005), 189—195.
CR  - 11. V. L., Singleton, R.Orthofer and R. M., Lamuela-Raventos, Analysis of Total Phenols and Other Oxidation Substrates and Antioxidants by Means of Folin-Ciocalteu Reagent, Methods in Enzymology, 299 (1999), 152-178.
CR  - 12. L. R., Fukumoto and G., Mazza, Assessing antioxidant and prooxidant activities of phenolic compounds, Journal Agriculture Food Chemistry, 48 (2000), 3597-3604.
CR  - 13. B., Trusheva, M., Popova, E. B., Koendhori, I., Tsvetkova, C., Naydenski, and V., Bankova, Indonesian propolis: Chemical composition, biological activity and botanical origin, Natural Product Research, 25 (2011), 606–613.
CR  - 14. J.S., Bonvehi, F.V., Coll, and R.E., Jorda, The Composition Active Components and Bacteriostatic Activity of Propolis in Dietetics, JAOCS, 71 (1994), 5, 18-25.
CR  - 16. V., Bankova, S., De Castro and M., Marcucci,  Propolis: recent advances in chemistry and plant origin, Apidologie, Springer Verlag, 31 (1) (2000), 3-15.
UR  - https://doi.org/10.15671/hjbc.593940
L1  - https://dergipark.org.tr/en/download/article-file/1051907
ER  -