TY  - JOUR
T1  - Evaluation of lung toxicity in rats kept in coal mine ambience by in vivo respiration records: An Experimental Study
TT  - Kömür madeni ortamında tutulan sıçanlarda akciğer toksisitesinin in vivo solunum kayıtları ile değerlendirilmesi: Deneysel Bir Çalışma
AU  - Arslan, Seyfullah Oktay
AU  - Çam, Saliha Ayşenur
PY  - 2019
DA  - August
Y2  - 2019
DO  - 10.30569/adiyamansaglik.596944
JF  - Adıyaman Üniversitesi Sağlık Bilimleri Dergisi
JO  - ADYÜ Sağlık Bilimleri Derg
PB  - Adıyaman University
WT  - DergiPark
SN  - 2458-9179
SP  - 1500
EP  - 1510
VL  - 5
IS  - 2
LA  - en
AB  - OBJECTIVE: Mineworkers exposed to dusts or toxic gasses in occupational atmosphericconditions. Pneumoconiosis and other lung diseases are charecterized by thepathogenesis of coal dust-caused pulmonary toxicity associated with reactiveoxygen species (ROS).MATERIALS AND METHODS:This study was conducted toinvestigate the respiration failures and fibrosis in rats after being exposedto coal dust and gases in mine atmosphere. Another aim was to study the therapeuticeffect of erdosteine as antioxidant therapy. Rats were exposed to mine ambiencefor four week, and then they were breathed in the clean air for four week. Therespiratory functions of rats were recorded once a week for eight week, as invivo. The fibrosis of lung tissue levels, the oxidant/antioxidant status, andcytokines of inflammation in bronchoalveolar lavage fluids (BALFs) wereevaluated, at the end of the processes.RESULTS: We observed to be the beginning of respiratoryabnormalities in animals exposed to coal dust in second week. The end of fourthweek, there were the increase of respiratory frequency and along with thedecrease of respiratory depth. The respiratory failures were not improved inclean ambience, moreover apnea were appearance in the end of six week (thesecond week of clean air). Deaths were 28% in animals. Erdosteine administer torats could not fully abolished to the pulmonary toxicity, however could able tohold to toxicity, and also there were not dies in rats administered toerdosteine. Coal dust exposure was resulted in fibrosis with higher hydroxyproline(HP) levels, cytokine inflammation with higher interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α)levels, and lipid peroxidation with an increased malondialdehyde (MDA) levels, accordingto the healthy. A dramatically run out of endogen antidote sulfide pools (GSH),and an increased MPO activity were dedected in the mine dusts and gassesexposure group, according to the healthy animals. High biochemical index oftoxicity were partly balanced by erdosteine.CONCLUSIONS: Our experimental findings support the hypothesisthat ROS is induced coal workers' pneumoconiosis. Re-oxygenation cannot be gettingit together to reverse the pulmonary toxicity. On the top of it, it can makeits pathogenesis further exaggerating and even worse. On this account we heartilyspeculate that re-oxygenation should be by steps in mine workers. In additionthe antioxidant therapy may be partly a choice be able to tolerate the coal dust-inducedlung toxicity of mine workers.
KW  - Respiratory functions
KW  - antioxidant therapy
KW  - 
KW  - re-oxygenation
KW  - oxidative stress
KW  - coal mine ambience
N2  - Amaç: Maden işçileri çalışma ortamıkoşullarında tozlara veya zehirli gazlara maruz kalmaktadır. Pnömokonyoz vediğer akciğer hastalıkları, kömür tozu kaynaklı pulmoner toksisitenin reaktifoksijen türleriyle (ROS) ilişkili patogenezi ile karakterizedir.Yöntem:Bu çalışma, maden atmosferinde kömür tozu ve gazlarına maruz kaldıktan sonrasıçanlarda solunum yetersizliği ve akciğer fibrozunu araştırmak için yapıldı.Diğer bir amaç, erdosteinin antioksidan tedavi olarak terapötik etkisiniaraştırmaktı. Sıçanlar, dört hafta boyunca maden ortamına maruz bırakıldı vedaha sonra dört hafta boyunca temiz havada kaldılar. Sıçanların solunumfonksiyonları, in vivo olarak, sekiz hafta boyunca haftada bir kez kaydedildi.İşlem sonunda, akciğer dokusu seviyelerinin fibrozisi, oksidan / antioksidandurumu ve bronkoalveoler lavaj sıvılarında (BALF) inflamasyonun sitokinlerideğerlendirildi.Bulgular:İkinci haftada kömür tozuna maruz kalan hayvanlarda solunum anormalliklerininbaşladığı gözlendi. Dördüncü haftanın sonunda, solunum sıklığının artması vesolunum derinliğinin azalması vardı. Solunum yetersizlikleri temiz ortamdaiyileşmedi, ayrıca altı haftanın sonunda (temiz havanın ikinci haftasında) apneortaya çıktı. Hayvanlarda ölümler % 28 idi. Ratlara uygulanan Erdostein,akciğer toksisitesini tamamen ortadan kaldıramadı, ancak toksisitedeki şiddetiazalttı ve ayrıca erdostein uygulanan sıçanlarda ölen yoktu. Kömür tozuna maruzkalma, yüksek hidroksiprolin (HP) seviyelerine sahip fibrozis, yüksekinterlökin-6 (IL-6) ve tümör nekroz faktörü-alfa (TNF-α) seviyelerinde sitokiniltihabı ve artmış malondialdehit (MDA) ile lipit peroksidasyonuyla sonuçlandı.Sağlıklı hayvanlara göre, endojen sülfit havuzları (GSH) dramatik bir şekildetükendi ve artmış bir MPO aktivitesi tespit edildi. Yüksek biyokimyasaltoksisite indeksi kısmen erdostein ile dengelenmiştir.Sonuç: Deneysel bulgularımız, ROS'unkömür işçilerinin pnömokonyozu oluşturduğu hipotezini desteklemektedir. Akciğertoksisitesini tersine çevirmek için yeniden oksijenasyon yarar sağlamaz.Üstelik, patogenezini daha da kötüleştiriyor. Bu nedenle, yenidenoksijenasyonun maden işçilerinde adım adım olması gerektiğini kuvvetledüşünüyoruz. Ek olarak, antioksidan tedavi, kısmen, maden işçilerinin kömürtozu kaynaklı akciğer toksisitesini tolere edebilmesi için bir seçenek olabilir.
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UR  - https://doi.org/10.30569/adiyamansaglik.596944
L1  - https://dergipark.org.tr/en/download/article-file/782956
ER  -