@article{article_597214, title={Characterization of plant growth-promoting traits and antagonistic potentials of endophytic bacteria from bean plants against Pseudomonas syringae pv. phaseolicola}, journal={Plant Protection Bulletin}, volume={59}, pages={59–69}, year={2019}, DOI={10.16955/bitkorb.597214}, author={Duman, Kamil and Soylu, Soner}, keywords={bean,halo blight,endophyte,antagonist,plant growth promoting bacteria,biological control,Pseudomonas syringae pv. phaseolicola}, abstract={<p class="MsoNormal" style="margin-top:6pt;margin-right:0cm;margin-bottom:6pt;margin-left:0cm;text-align:justify;line-height:150%;"> <i> <span style="font-family:Arial, sans-serif;" lang="en-us" xml:lang="en-us">Pseudomonas syringae </span> </i> <span style="font-family:Arial, sans-serif;" lang="en-us" xml:lang="en-us">pv. <i>
phaseolicola </i>( <i>Psp </i>) is a
seed-borne bacterium which causes halo blight disease in common bean. This
study aimed the determination of plant growth-promoting traits (such as IAA,
phosphate solubilization) and antagonistic potentials (such as siderophore and ammonia
productions) of endophytic plant growth-promoting bacteria (PGPB) from healthy
bean plants growing in different regions against <i>Psp in vitro </i>conditions. A total of 131 bacteria were primarily
characterized as putative PGPB and tested for antagonist activity against <i>Psp </i> in dual culture tests. Seventy-one
bacterial isolates demonstrated antagonistic activities against <i>Psp </i> isolate in varying ratios. Among
these 71 isolates, 30 representative bacterial isolates from the different
regions/fields were selected. On the basis of morphological, physiological,
biochemical characteristics and confirmation by MALDI-TOF analyses, 30
endophytic antagonist isolates were identified as belonging to 10 genera, 24
different species. According to results obtained, 10 isolates belong to <i>Bacillus </i> spp., 6 isolates belong to <i>Pseudomonas </i> spp., 4 isolates belong to <i>Rhizobium radiobacter </i>, 2 isolates belong
to <i>Arthrobacter </i> spp., 2 isolates
belong to <i>Achromobacter spanius </i>, <i> </i>2 isolates belong to <i>Serratia </i> <i>liquefaciens </i>, 1 isolate belongs to <i>Acinetobacter </i> <i>calcoaceticus </i>, <i> Exiguobacterium </i>sp <i>. </i>, <i> Microbacterium </i> <i>hydrocarbonoxydans </i>, and <i>Ochrobactrum anthropi </i>. The largest and
lowest inhibition zone was produced by endophytic bacterial isolates <i>Pseudomonas gessardii </i> (4.85) and <i>Bacillus licheniformis </i> (1.35). Among the
tested antagonist bacterial isolates, 10 isolates were positive for the
production of α-amylase, 7 isolates positive for phosphate solubilization, 29
isolates positive for siderophore production, 11 isolates positive for protease
production. All selected bacterial isolates produced IAA and ammonia in
relatively varying amounts. <i>P. gessardii </i>
produced a relatively large amount of extracellular siderophore (5.83), <i>Exiguobacterium </i> sp. produced a
relatively large amount of extracellular protease (5.25), <i>P. gessardii </i> and <i>O. anthropi </i>
produced a relatively large amount of extracellular IAA (161.39 µg/ml) and <i>Acinetobacter calcoaceticus </i> produced a
relatively large amount of phosphatase (2.63). This is the first study reporting
bean plants harbor endophytes having plant growth promoting activities with
antagonistic potential against <i>Psp </i>. </span> </p> <p class="MsoNormal" style="margin-top:6pt;margin-right:0cm;margin-bottom:6pt;margin-left:0cm;text-align:justify;line-height:150%;"> <br /> </p> <p> <br /> </p>}, number={3}, publisher={TARIM VE ORMAN BAKANLIĞI EĞİTİM VE YAYIN DAİRESİ BAŞKANLIĞI}, organization={Tarımsal Araştırmalar ve Politikalar Genel Müdürlüğü, Mustafa Kemal Üniversitesi Fen Bilimleri Enstitüsü}