@article{article_597942, title={Investigation of D-e-MAPP-derived Cytotoxicity on Human Prostate Cancer Cells}, journal={Bilecik Şeyh Edebali Üniversitesi Fen Bilimleri Dergisi}, volume={6}, pages={204–212}, year={2019}, author={Kutlu, Mehtap and İzgördü, Hüseyin and Çömlekçi, Emre and Vejselova Sezer, Canan}, keywords={Sphingolipid,DU-145,Confocal microscopy}, abstract={<p> <span class="IEEEAbstractHeadingChar"> <span lang="en-us" style="font-size:9pt;" xml:lang="en-us">Sphingolipids play critical role in
biological processes such cell death, survival and drug resistance in many
cancers. Ceramide and dihydroceramide are proliferation and death associated
sphingolipids. Recent cancer research are focused on clarifying
cancer-sphingolipid metabolism. In last years, ceramide as a key molecule in
sphingolipid metabolism relationship has been investigated for its anticancer
activity via augmenting its intracellular level by ceramidase inhibitor
application. Prostate cancer is amoung the most frequent human cancers and is
reported as second most common cancer-related death cause. Prostate cancer is
common at ages older than 65. The aim of this study was to investigate the
potential cytotoxic activity of a ceramidease inhibitor
(1S,2R)-D-erythro-2-(N-Myristoylamino)-1-phenyl-1-propanol (D-erythro-MAPP) on
human prostate cancer DU-145 cells by using MTT
(3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) assay, flow
cytometry, confocal and TEM microscopy. MTT findings showed that D-erythro-MAPP
caused toxicity in low doses on DU-145 cells. Confocal and TEM microscopy
findings indicated hole formation in cytoskeleton, chromatin condensation,
horseshoe-shaped cell nuclei as morphological changes and blebbings on cell
membrane, fragmentation of nuclei, chromatin condensation and loss of criastae
of as ultrastructural changes, respectively. Flow cytometry findings showed
that D-erythro-MAPP triggered apoptosis in short term application of 24 hours
on DU-145 cells. According to results it can be conculded that D-erythro-MAPP
decreased viability of DU-145 cells in dose-dependent manner.  Our findings stated the anti-cancer and
cytotoxic potential of D-erythro-MAPP on DU-145 and this agent might be used in
drug developing for cancer treatment after the further in vitro and in vivo
studies. </span> </span> <br /> </p>}, publisher={Bilecik Seyh Edebali University}