@article{article_604797, title={The Effects of Thyroid Hormones on Glucokinase Enzyme Activity in diabetic Rat Liver Tissues}, journal={Tıp Fakültesi Klinikleri Dergisi}, volume={2}, pages={105–112}, year={2019}, author={Şahin, Duygu and Altan, Nilgün and Sepici-dinçel, Aylin and Engin, Atilla}, keywords={Glucokinase,Thyroid hormone,Diabetes Mellitus,Rat liver}, abstract={<p class="MsoNormal" style="margin:0cm 0cm .0001pt 35.4pt;text-align:justify;text-indent:35.4pt;line-height:150%;"> <span style="font-family:’TimesNewRomanPS-BoldMT’;font-size:10pt;color:rgb(4,5,3);"> <b>Objective: </b> <span style="font-family:TimesNewRomanPSMT;font-size:10pt;">We aimed to investigate the effcts of insulin and various doses of thyroid hormones  <span style="font-size:10pt;">with combine treatment on glucokinase (GK) enzyme activity, which is the one of the key  <span style="font-size:10pt;">enzyme in carbohydrate metabolism, diabetes and hypothyroidism.  </span> </span> </span> </span> </p> <p class="MsoNormal" style="margin:0cm 0cm .0001pt 35.4pt;text-align:justify;text-indent:35.4pt;line-height:150%;"> <span style="font-family:’TimesNewRomanPS-BoldMT’;font-size:10pt;color:rgb(4,5,3);"> <span style="font-family:TimesNewRomanPSMT;font-size:10pt;"> <span style="font-size:10pt;"> <span style="font-size:10pt;"> <span style="font-family:’TimesNewRomanPS-BoldMT’;font-size:10pt;"> <b>Material and methods: </b> <span style="font-family:TimesNewRomanPSMT;font-size:10pt;">Rats were assigned to eight groups: Group 1: control, Group 2: diabetes  <span style="font-size:10pt;">(DM), Group 3: DM + insulin, Group 4: thyroidectomized control, Group 5: thyroidectomized +  <span style="font-size:10pt;">DM, Group 6: thyroidectomized + DM + insulin, Group 7: thyroidectomized + DM + insulin +  <span style="font-size:10pt;">thyroid hormone (TH) (2.5μg/kg), Group 8: thyroidectomized + DM + insulin + TH (5 μg/kg).  <span style="font-size:10pt;">Glucose concentration, HbA1c and thyroid hormone levels were measured in blood samples and  <span style="font-size:10pt;">GK enzyme activities were determined in liver tissue samples. </span> </span> </span> </span> </span> </span> </span> </span> </span> </span> </span> </p> <p class="MsoNormal" style="margin:0cm 0cm .0001pt 35.4pt;text-align:justify;text-indent:35.4pt;line-height:150%;"> <span style="font-family:’TimesNewRomanPS-BoldMT’;font-size:10pt;color:rgb(4,5,3);"> <span style="font-family:TimesNewRomanPSMT;font-size:10pt;"> <span style="font-size:10pt;"> <span style="font-size:10pt;"> <span style="font-family:’TimesNewRomanPS-BoldMT’;font-size:10pt;"> <span style="font-family:TimesNewRomanPSMT;font-size:10pt;"> <span style="font-size:10pt;"> <span style="font-size:10pt;"> <span style="font-size:10pt;"> <span style="font-size:10pt;"> <span style="font-size:10pt;"> <span style="font-family:’TimesNewRomanPS-BoldMT’;font-size:10pt;"> <b>Results: </b> <span style="font-family:TimesNewRomanPSMT;font-size:10pt;">Our results showed that the glucokinase enzyme activities were significantly decreased  <span style="font-size:10pt;">in diabetic rat liver tissues compared to control group. Moreover it was observed that the enzyme  <span style="font-size:10pt;">activities were slightly regulated by insulin. It is demonstrated that decreased GK enzyme  <span style="font-size:10pt;">activity in thyroidectomized diabetic rat liver was regulated by insulin and various doses of  <span style="font-size:10pt;">thyroid hormones. </span> </span> </span> </span> </span> </span> </span> </span> </span> </span> </span> </span> </span> </span> </span> </span> </span> </p> <p class="MsoNormal" style="margin:0cm 0cm .0001pt 35.4pt;text-align:justify;text-indent:35.4pt;line-height:150%;"> <b style="font-family:’TimesNewRomanPS-BoldMT’;font-size:10pt;color:rgb(4,5,3);text-indent:35.4pt;">Conclusion: </b> <span style="font-size:10pt;color:rgb(4,5,3);text-indent:35.4pt;font-family:TimesNewRomanPSMT;">As a result the possible contribution of thyroid hormones to insulin effct to  </span> <span style="font-size:10pt;font-family:TimesNewRomanPSMT;color:rgb(4,5,3);text-indent:35.4pt;">normalize diabetic induced changes in liver tissue has been shown. </span> </p>}, number={3}, publisher={Istanbul Aydin University}