@article{article_612768, title={Association Between Antibiotic Resistance and Biofilm Formation of Escherichia coli Strains Isolated from Blood Culture}, journal={Experimed}, volume={9}, pages={60–64}, year={2019}, author={Halipçi Topsakal, Hatice Nur and Aydoğan, Okan and Özdemir, Sinem and Köksal Çakırlar, Fatma}, keywords={Escherichia coli,biofilm,ESBL,carbapenemase,antibiotic resistance,hemoculture}, abstract={<p class="MsoNormal"> <span lang="EN-US"> </span> </p> <p class="MsoNormal" style="text-align: justify; "> <span lang="EN-US"> <b>DOI </b>: 10.26650/experimed.2019.19013 <o:p> </o:p> </span> </p> <p class="MsoNormal" style="text-align: justify;"> <span lang="EN-US"> <br> </span> </p> <p class="MsoNormal" style="text-align: justify; "> <span lang="EN-US"> <b>Objective </b>: Bloodstream infections (BSIs) appear to be important causes of morbidity and mortality. Escherichia coli (E. coli) causes various serious diseases such as BSIs. The emergence of multidrug resistance in E. coli has become a global concern. E. coli has the capability to colonize and survive on several surfaces in different time periods.  This is achieved by adhering to inert and cellular substrates and forming a biyofilm layer. The aim of this study is to investigate the relationship of biyofilm formation to antibiotic resistance in E. coli isolates which are isolated from hemocultures. <o:p> </o:p> </span> </p> <p class="MsoNormal" style="text-align: justify; "> <span lang="EN-US"> <b>Material and Method </b>: E. coli strains were isolated from blood samples of patients with bacteremia who were hospitalized in intensive care units and in other departments of Istanbul University-Cerrahpaşa, Cerrahpaşa School of Medicine Hospital. Blood cultures were analyzed with the Bactec 9120 system (Becton Dickinson, USA). The identification and antimicrobial resistance of 62 E. coli strains were determined by the Phoenix automated system (BD Diagnostic Systems, Sparks, MD) according to the European Committee on Antimicrobial Susceptibility Testing (EUCAST) and the Clinical and Laboratory Standard Institute (CLSI). Biofilm formation was determined by the Congo red agar method. <o:p> </o:p> </span> </p> <p class="MsoNormal" style="text-align: justify; "> <span lang="EN-US"> <b>Results </b>: Biofilm formation was detected in 42 (67.7%) of the sixty-two E. coli isolates. Extended-spectrum beta-lactamase (ESBL) production was 38 (61.2%) and carbapenemase production was 12 (19.3%). 28 (66.6%) of the biofilm-positive isolates were ESBL-positives and 10 (23.8%) were carbapenemase-positives. Nine isolates were both ESBL and carbapenemase positives. Ten isolates were biofilm, ESBL and carbapenemase were positive at all. <o:p> </o:p> </span> </p> <p class="MsoNormal"> <span lang="EN-US"> </span> </p> <p class="MsoNormal" style="text-align: justify; "> <span lang="EN-US"> <b>Conclusion </b>: Resistance rates against various antibiotics in E. coli strains isolated from blood cultures were found higher in biyofilm positive strains than in negative strains at Istanbul University-Cerrahpaşa, Cerrahpaşa School of Medicine Hospital. <o:p> </o:p> </span> </p> <p class="MsoNormal" style="text-align: justify; "> <span lang="EN-US"> <b>Cite this article as </b>: Halipçi Topsakal HN, Aydoğan O, Özdemir S, Köksal Çakırlar F. Association Between Antibiotic Resistance and Biofilm Formation of Escherichia coli Strains Isolated from Blood Culture. Experimed 2019; 9(2): 60-4. <o:p> </o:p> </span> </p>}, number={2}, publisher={Istanbul University}