TY  - JOUR
T1  - Micropropagation of Turkestan Soap Root Allochrusa gypsophiloides – Natural Source of Saponins
TT  - Micropropagation of Turkestan Soap Root Allochrusa gypsophiloides – Natural Source of Saponins
AU  - Mursalıyeva, Valentina
AU  - Imanbayeva, Akjunus
PY  - 2020
DA  - March
DO  - 10.21448/ijsm.627140
JF  - International Journal of Secondary Metabolite
JO  - Int. J. Sec. Metabolite
PB  - İzzet KARA
WT  - DergiPark
SN  - 2148-6905
SP  - 1
EP  - 7
VL  - 7
IS  - 1
LA  - en
AB  - Allochrusa gypsophiloides or Turkestan soap root (TSR), which is endemicto Central Asia, is a valuable saponin-bearing technical and medicinal plant.The purpose of this study was to develop invitro mass propagation for the conservation of endangered species. Nodeexplants were induced to regenerate adventitious shoot buds on Murashige andSkoog medium (MS) supplemented with different concentrations of6-benzylaminopurine (BAP) and/or kinetin in combination with naphtylacetic acid(NAA). The maximum number of shorter shoots per explant (16.8 ± 3.1) wasrecorded on MS contained 0.5 mg/L BAP and 0.5 mg/L NAA after one monthcultivation. For elongation, obtained shoot conglomerates were transfered forhormone-free MS. The cultivation of initial explants on MS with kinetin led toa three-fold reduction in the number of shoots characterized by a maximumlength and clearly defined internodes (without the stage of elongation).Micropropagation was achieved by cutting obtained shoots and adventitious shootinduction. The maximal shoot proliferation (62 ± 6.9) on MS 0.5 mg/L BAP + 1.0mg/L kinetin + 0.5 mg/L NAA was obtained. Shoots of about 0.5 cm required toelongation before rooting on the liquid ½ MS medium contained NAA or IBA. Inboth cases, auxin concentration 2.0 mg/L induced maximal rooting (58 % and 60%, respectively) at 20-day’s incubation. Type of auxin was influenced most onroot quality (thickness, color, branching) than on their frequency and number.
KW  - Allochrusa gypsophiloides
KW  - Turkestan soaproot
KW  - Saponin-bearing plant
KW  - Adventitious shoot
KW  - Micropropagation
N2  - Allochrusa gypsophiloides or Turkestan soap root (TSR), which is endemic to Central Asia, is a valuable saponin-bearing technical and medicinal plant. The purpose of this study was to develop in vitro mass propagation for the conservation of endangered species. Node explants were induced to regenerate adventitious shoot buds on Murashige and Skoog medium (MS) supplemented with different concentrations of 6-benzylaminopurine (BAP) and/or kinetin in combination with naphtylacetic acid (NAA). The maximum number of shorter shoots per explant (16.8 ± 3.1) was recorded on MS contained 0.5 mg/L BAP and 0.5 mg/L NAA after one month cultivation. For elongation, obtained shoot conglomerates were transfered for hormone-free MS. The cultivation of initial explants on MS with kinetin led to a three-fold reduction in the number of shoots characterized by a maximum length and clearly defined internodes (without the stage of elongation). Micropropagation was achieved by cutting obtained shoots and adventitious shoot induction. The maximal shoot proliferation (62 ± 6.9) on MS 0.5 mg/L BAP + 1.0 mg/L kinetin + 0.5 mg/L NAA was obtained. Shoots of about 0.5 cm required to elongation before rooting on the liquid ½ MS medium contained NAA or IBA. In both cases, auxin concentration 2.0 mg/L induced maximal rooting (58 % and 60 %, respectively) at 20-day’s incubation. Type of auxin was influenced most on root quality (thickness, color, branching) than on their frequency and number.
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CR  - Gemejiyeva, N., Mursaliyeva, V., Mukhanov, T. (2016). Assessment of the current state of Allochrusa gypsophiloides (Regel) Schischk. natural populations in the South-Kazakhstan region. News of Nation. Acad. Sci. Rep. of Kazakhstan. Ser. Biol. and Medic., 1(313), 22-29. ISSN 2224-5308.
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UR  - https://doi.org/10.21448/ijsm.627140
L1  - https://dergipark.org.tr/en/download/article-file/955351
ER  -