@article{article_635833, title={Protective effect of lycopene on diethylnitrosamine-induced oxidative stress and catalase expression in rats}, journal={Ankara Üniversitesi Veteriner Fakültesi Dergisi}, volume={66}, pages={43–52}, year={2018}, author={Kaya, Emre and Yılmaz, Seval and Çeribaşı, Ali Osman and Telo, Selda}, keywords={Antioxidant,CAT expression,diethylnitrosamine,lycopene,oxidative stress}, abstract={<span class="fontstyle0">The purpose of this study is to investigate the protective role of lycopene on diethylnitrosamine (DEN)-induced <br>hepatotoxicity using biochemical and histopathological approaches. The rats were divided into 5 groups as control, lycopene, DEN, <br>lycopene+DEN and DEN+lycopene. DEN was administered to rats at 200 mg/kg, a single dose intraperitoneally for 30 days. Lycopene <br>was administered to rats every other day at 10 mg/kg, gavage for 10 days. Lycopene administration was started 10 days before the <br>DEN administration in lycopene+DEN group and together with the DEN administration in DEN+lycopene group. In this study, <br>malondialdehyde (MDA), reduced glutathione (GSH) levels, catalase (CAT), glutathione peroxidase (GSH-Px), glutathione-Stransferase (GST), superoxide dismutase (SOD) activities and the expression levels of the CAT enzyme were measured in blood and <br>liver tissues. DEN caused the oxidative stress by the increased MDA level and the reduced GSH level, antioxidant enzyme activities <br>in tissues. Lycopene administration produced amelioration in biochemical indices of hepatotoxicity in both blood and liver tissues <br>when compared to DEN group; simultaneous-administration with DEN has been more effective. It was determined to increase <br>expression levels of the CAT enzyme in the DEN group in RT-PCR. The improvement in expression levels in DEN+lycopene group <br>was observed to be better than the lycopene+DEN group. Histopathologically, many different histopathological changes were observed <br>in liver tissues of DEN and lycopene+DEN groups, it was determined that these changes reduced in DEN+lycopene group. The results <br>from the present study indicate that lycopene exhibits good hepatoprotective and antioxidant potential against DEN-induced <br>hepatocellular damage in rats. </span>   <br style="font-variant-numeric: normal; font-variant-east-asian: normal; line-height: normal; text-align: -webkit-auto; text-size-adjust: auto;">}, number={1}, publisher={Ankara University}