@article{article_638403, title={Simultaneous Determination of Ascorbic Acid, Paracetamol, Aspirin in Tablets Using UPLC}, journal={Hacettepe University Journal of the Faculty of Pharmacy}, pages={135–149}, year={2016}, author={Selimoğlu, Faysal and Kadıoğlu, Yücel and Dinç, Erdal}, keywords={Quantitative resolution, UPLC method, Paracetamol, Ascorbic acid, Aspirin, Pharmaceutical preparation}, abstract={Ultra-high-performance liquid chromatographic UPLC data obtained from photodiode array PDA detection was processed by the PCR and PLS algorithms for the simultaneous quantitative resolution of Ascorbic Acid AA , Paracetamol PAR , Aspirin ASP in a commercial formulation. Principle component regression PCR and partial least squares PLS were applied to the peak area ratio of the analytes/internal standard at multi-wavelength PDA detector responses. The combined use of UPLC and chemometric calibration techniques was denoted UPLC- PCR and UPLC-PLS. For the comparison purpose, the UPLC method was used for the confirmation of the results obtained from combined UPLC-chemometric calibration techniques. A good chromatographic separation between drugs and internal standard IS was achieved using a Waters ACQUITY UPLC BEH Phenyl 100 mm x 1.0 mm, i.d., 1.7 μm column and a mobile phase consisting of 0.1 M CH3COOH and methanol 75:25, v/v . The multi-wavelength PDA detection for Ascorbic Acid AA , Paracetamol PAR , Aspirin ASP was accomplished by measuring the peak area at the wavelength set corresponding to 245, 250, 255.0, 260.0, 265, 270, 275 and 280 nm. The proposed UPLC-PCR and UPLC-PLS approaches were validated by using the synthetic mixtures, and inter-day and intra-day experiments. The proposed methods were successfully applied to commercial samples containing the analyzed drugs.}, number={2}, publisher={Hacettepe University}