TY - JOUR TT - Novel Support Material with Polymeric Ligand for Ion Exchange Chromatography: Synthesis, Characterization and Chromatographic Performance AU - Saraçoğlu Kaya, Berna AU - Tuncel, Ali PY - 2019 DA - December JF - International Journal of Multidisciplinary Studies and Innovative Technologies JO - IJMSIT PB - SET Teknoloji WT - DergiPark SN - 2602-4888 SP - 173 EP - 183 VL - 3 IS - 2 LA - tr KW - Free Radical Grafting Polymerization (FRGP) KW - High Performance Liquid Chromatograpy (HPLC) KW - Ion Exchange Chromatography (IEC) KW - Monodisperse and porous polymeric particles N2 - Within the scope of the study, polymeric ligandswith weak cation exchange carboxyl (-COOH) groups formed on the surface,designed for High Performance Liquid Chromatography (HPLC), monodisperse andporous poly(2,3-dihydroxypropyl methacrylate-co-ethylene dimethacrylate),poly(DHPM- co-EDM) particles were tested in Ion Exchange Chromatography (IEC)mode for chromatographic separation of protein mixtures as a weak cationexchange column-material. The synthesis of theparticles obtained for the design of the column support material, which is thefirst step of the study carried out in three stages, takes place in fourdifferent sections. Firstly, monodispersepolystyrene microgels to form the skeletal structure were synthesized by “DispersionPolymerization”, then monodispersed-porous poly(glycidyl methacrylate-co-ethyleneglycoldimethacrylate), poly(GMA-co-EDM) microspheres with epoxy groups were producedby using “multi-stage microsuspension polymerization”. The particles were hydrolyzed in acidic mediumto form the surface in diol form and poly(2,3-dihydroxypropylmethacrylate-co-ethylene dimethacrylate), poly(DHPM-co-EDM) particles wereobtained which were suitable for chromatographic use. The particles obtained in the last step wereused in the “Free Radical Grafting Polymerization” (FRGP) method for theproduction of polymeric ligand containing carboxyl group. Poly (DHPM-co-EDM) particles with –OH groups onthe surface are silanated with 3-trimethoxysilylpropyl methacrylate (TMSPM) andderivatized with methacrylic acid (MAA) to form poly(MAA) ligand on the surfaceof poly(DHPM-c-EDM) particles were obtained for ion-exchange chromatography. The characterization and chromatographicperformance of the particles were investigated in the second and third stagesof the study. For particlessynthesized by the FRGP method, the average-pore size was 33 nm and thespecific-surface area was 21 m2/g. Theoretical plate height values ​​between 200-320 µm were obtained for weakcation exchange column. The peak resolution values ​​in the chromatogram werefound to be above 1.4 for separation as short as 15 minutes. Protein recoveryvalues ​​were found to be between 90-100% for all proteins. Forthe produced poly(MAA)-grafted-poly(DHPM-co-EDM) column, the BSS values ​​ofthe analytes from analysis to analysis and from day to day reproducibilitytests were below 1%. 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