@article{article_656701, title={Determination of Seroprevalence of Infectious Bovine Keratoconjunctivitis Disease by ELISA and AGID}, journal={Atatürk Üniversitesi Veteriner Bilimleri Dergisi}, volume={15}, pages={106–112}, year={2020}, DOI={10.17094/ataunivbd.656701}, author={Ataseven, Lale}, keywords={Agar gel immunodiffusion, ELISA, Moraxella bovis, Seroprevalence, Turkey}, abstract={Infectious Bovine Keratoconjunctivitis (IBK) is a highly contagious disease of conjunctiva and cornea in cattle which causes significant economic losses in cattle breeding. The objective of this study is to determine serum antibodies against M. bovis and obtaining information about the seroprevalence of IBK by AGID test and ELISA. Serum samples, collected from 552 cattle in different herds in Turkey were examined for specific antibodies against Moraxella bovis (M. bovis). The seropositivity of IBK in cattle sera with clinical signs was determined as 26.1% and 5.8% by ELISA and AGID, respectively. Seropositivity in sera samples of healthy appeared cattle were detected as 26.3% and 5.6% by ELISA and AGID, respectively. According to the results, the highest seropositivity (36.5%) was detected in the animals aged 12-24 months. Similar seroprevalence detection rates were recorded in cattle with and without clinical signs. Clinical signs, spesifically, does not give knowledge about the exact percentage of the IBK. Therefore, besides isolation and identification of the agent, serological tests like ELISA, as a screening test, should be preferred to determine the presence and spread of IBK in the herd.}, number={2}, publisher={Ataturk University}