@article{article_681611, title={AN INVESTIGATION OF THE PHARMACOKINETICS AND POTENTIAL METABOLITES OF POTASSIUM 2-((4-AMINO-5- (MORFOLINOMETYL)-4H-1,2,4-TRIAZOL-3-YL)THIO) ACETATE ON RATS}, journal={Journal of Faculty of Pharmacy of Ankara University}, volume={44}, pages={233–241}, year={2020}, DOI={10.33483/jfpau.681611}, author={Shcherbyna, Roman}, keywords={1.2.4-triazole, metabolite, pharmacokinetics}, abstract={<div style="text-align:justify;"> <span style="font-size:12px;"> <b>Objective: </b> <i>The purpose of this work is to study the designated pharmacokinetic parameters and possible metabolites of the substance potassium 2-((4-amino-5-(morfolinometyl)-4H-1,2,4-triazol-3-yl)thio)acetate by intragastric introduction to rats. </i> </span> </div> <div style="text-align:justify;"> <span style="font-size:12px;"> <b>Material and Method: </b> <i>The study was performed on Wistar rats. The following parameters were calculated to evaluate the pharmacokinetic properties: area under the pharmacokinetic curve (AUC), apparent clearance (CL), elimination rate constant (K), volume of distribution (VT), serum half-life (t1⁄2). Separation was performed using Agilent 1260 Infinity HPLC system. </i> </span> </div> <div style="text-align:justify;"> <span style="font-size:12px;"> <b>Result and Discussion: </b> <i>The determination of the concentration of potassium 2-((4-amino-5-(morfolinometyl)-4H-1,2,4-triazol-3-yl)thio)acetate in serum was performed for 0.08 (5 min), 0.25 (15 min), 0.75 (45 min), 2.5, 7.5, 13.5 and 24 hours. It was found that in the serum of rats the maximum concentration of the injected substance was reached almost immediately after administration, namely for 5 min with an average value at the level of 279,67 µg/ml. Thereafter, the concentration decreases mono-exponential, which indicates that the distribution and elimination occur at the same rate. The area under the pharmacokinetic curve is AUC = 150,8998 µg*h/ml. The serum half-life of the specified substance is established (t1⁄2 = 0.32 hours). Based on the obtained chromatograms and mass spectra, 5 possible metabolites of potassium 2-((4-amino-5-(morfolinometyl)-4H-1,2,4-triazol-3-yl)thio)acetate (PKR-173)were determined by intragastric introduction to rats. </i> </span> </div>}, number={2}, publisher={Ankara University}