@article{article_709924, title={Cytotoxicity and Collagen Expression Effects of Tideglusib Administration on Human Periodontal Cells: An In-Vitro Study}, journal={Clinical and Experimental Health Sciences}, volume={10}, pages={153–162}, year={2020}, DOI={10.33808/clinexphealthsci.709924}, author={Oncu, Buse and Yilmaz, Ayse and Karademir, Betul and Altunok, Elif Çiğdem and Kuru, Leyla and Ağralı, Ömer Birkan}, keywords={Cell biology,Cell signaling biomolecules,Osteoblast(s),Tideglusib,Wnt/β-catenin signaling pathway}, abstract={<div style="text-align:justify;"> <b>Objective: </b> Tideglusib is a GSK-3 inhibitor activating Wnt/β-catenin signaling pathway which has significant importance in regenerative response. <br />The aim of this study was to evaluate the cytotoxicity and protein expression impacts of Tideglusib on human periodontal cell lines. </div> <div style="text-align:justify;"> <br /> <b>Methods: </b> Cytotoxicity effect of different concentrations (50nM, 100nM, 200nM) of Tideglusib application on human gingival fibroblast (hGF), <br />periodontal ligament fibroblast (hPDLF), and osteoblast (hOB) cell lines was determined. Type-I and III collagen expressions were evaluated <br />after 24-hour application of 50nM Tideglusib. </div> <div style="text-align:justify;"> <br /> <b>Results: </b> The cytotoxicity of 200nM Tideglusib was higher in hGF and hOB (p<0.05), but no difference was found in hPDLF compared to the <br />respective control group (p>0.05). The hGF and hOB treated with 50nM Tideglusib expressed an increased level of Type-I collagen (p<0.05), but <br />no difference was detected in the hPDLF compared to the respective control (p>0.05). Type-III collagen expressions were similar between the <br />test and control groups for each cell line (p>0.05). </div> <div style="text-align:justify;"> <br /> <b>Conclusion: </b> Tideglusib is not cytotoxic at 50nM and 100nM concentrations and may have positive effect on bone regeneration rather than <br />periodontal regeneration since it stimulated Type-I collagen production in hGF and hOB cells, but not in hPDLF. <br /> </div>}, number={2}, publisher={Marmara University}, organization={This study was supported by a grant from Marmara University Scientific Research Project Commission, Istanbul, Turkey.}