@article{article_735544, title={Investigation of the Effects of Topical Centarium Erythraea in Full-Thickness Skin Wounds in Diabetic Rabbits}, journal={Dicle Üniversitesi Veteriner Fakültesi Dergisi}, volume={13}, pages={92–98}, year={2020}, DOI={10.47027/duvetfd.735544}, author={Yavuz, Ünal and Temamoğulları, Füsun and Yığın, Akin and Yumuşak, Nihat}, keywords={Centarium erythraea,Centella asiatica,gene expression,rabbit,wound healing}, abstract={Centarium erythraea, a species of flowering plant in the Gentianaceae family, is a plant commonly used in the wound treatment. This study aims to investigate the effect of Centarium erythraea in enhancing healing process in full-thickness skin wounds in diabetic rabbits. A total of 28 young (6 months and body weight of 2.5-3 kg) female New Zealand rabbits were used. Diabetes mellitus was created by injection of alloxan monohydrate. After 15 days following diabetes induction, the surgical operation was applied under general anesthesia and two full-thickness 2.6 x 2.6 cm wounds were created that were equidistant from the dorsal median line. A total of four groups were formed with 7 rabbits in each group. For wounds, on a daily basis, oily homogenized form of Centarium erythraea was administered in Group 1, while pomade form of the titrated extract of Centella asiatica in Group 2, pure olive oil in Group 3 and normal saline was applied in Group 4. Wound edges were measured on the 4, 8, 12, 16, 20 and 24th days, furthermore, histopathological and genetic examinations were performed on tissue samples taken on the same days. Examining the wound diameters, healing rates in the Group 1 and Group 2 were found statistically significant compared to Group 3 and Group 4 (p <0,05). In histopathological examinations, it was noted that connective tissue increase, collagen proliferation and epitelogenesis were significant in Groups 1 and 2 compared to Groups 3 and 4 (p <0.05). Inflammatory cell infiltrations, hyperemia and necrosis in Groups 3 and Group 4 were observed with a high intensity compared to Groups 1 and Group 2 (p <0.05). IL-6, IL-8 and CXCR1 gene expressions were observed to be low in all diabetic groups. Although the expression values were low in groups 1 and 2, it was found that there was a significant difference in the expression amounts of the target genes on the 4 and 8 days compared to other groups (p <0.05). As a result, when the healing rate in the full-thickness skin wounds in the diabetic rabbits is examined at the level of wound diameters, histopathology and gene expression, it occurred in order of Group 2, Group 1, Group 3 and Group 4. Oily homogenized form of Centarium erythraea was found to have a positive effect in full-thickness wound healing of diabetic rabbits.}, number={2}, publisher={Dicle University}