TY  - JOUR
T1  - Optimization and Selection of Mobile Phase for the Determination of Multiple Pesticide Standards Using Liquid Chromatography-Tandem Mass Spectrometry
AU  - Lawal, Abubakar
AU  - Wong, Richard Chee Seng
PY  - 2021
DA  - February
Y2  - 2021
DO  - 10.18596/jotcsa.778786
JF  - Journal of the Turkish Chemical Society Section A: Chemistry
JO  - JOTCSA
PB  - Turkish Chemical Society
WT  - DergiPark
SN  - 2149-0120
SP  - 263
EP  - 278
VL  - 8
IS  - 1
LA  - en
AB  - The selection of the best mobile phase setup is one of the most important factors to be considered prior to quantitative instrumentation of multiple pesticides. Usually, mobile phases comprises of water (A) and an organic solvent (B) are the setup used in liquid chromatography instruments for the analysis of pesticide residues in various samples. Unfortunately, most of the analysis are being carried out without optimization and selection of the best mobile phase setup to improve the sensitivity of the instrument. For that reason, the comparative analysis of the reportedly used mobile phases and some few suggested ones was carried out on the multi-pesticide mixture of 0.1 mg/kg (100 µg/kg) standard solutions and quantified with liquid chromatography-tandem mass spectrometry (LC-MS/MS) instrument. Consequently, the best mobile phases setup that resulted in the sum of average total chromatographic peak areas (ATCPAs) and average total chromatographic peak heights (ATCPH) for the total ion chromatography (TIC) scans as an index that correspond to the concentration levels was selected [0.1 % formic acid in H2O (A) and 0.1 % formic acid in acetonitrile (ACN) (B)]. And further optimization was successfully carried out on the selected mobile phase A and the resulted setup [1 % ACN and 0.1 % formic acid in Milli-Q-water (mobile phase A) coupled with 0.1 % formic acid in ACN (mobile phase B)] improved the instrumental sensitivity on the targeted analytes. Thus, this justify the potential benefits of optimizing setup of the mobile phases prior to LC-MS/MS instrumentation of multi-pesticide analytes.
KW  - Mobile phase
KW  - Analysis of multi-pesticide residues
KW  - Liquid chromatography-tandem mass spectrometry
KW  - Total ion chromatography
KW  - Total chromatographic peak areas
UR  - https://doi.org/10.18596/jotcsa.778786
L1  - https://dergipark.org.tr/en/download/article-file/1233955
ER  -