TY  - JOUR
T1  - Çakalburnu Lagünü (İzmir) sedimentlerinde real-time PCR ile bakteri ve arke düzeylerinin değerlendirilmesi
TT  - Assessment of bacteria and archaea levels in Çakalburnu Lagoon (İzmir) sediments by real-time PCR
AU  - Kaçar, Aslı
AU  - Omuzbüken, Burcu
PY  - 2021
DA  - June
DO  - 10.12714/egejfas.38.2.02
JF  - Ege Journal of Fisheries and Aquatic Sciences
JO  - EgeJFAS
PB  - Ege University
WT  - DergiPark
SN  - 2148-3140
SP  - 147
EP  - 154
VL  - 38
IS  - 2
LA  - tr
AB  - Kıyısal lagünler, bir veya daha fazla sayıda giriş ile su değişimine izin veren ve bir bariyer gibi denizden ayrılan sığ su kütleleridir. Bu kırılgan ekosistemler kendilerine özgü sediment yapılarına sahip olmaktadır. Büyük ölçüde bentik mikrobiyal döngü yoluyla yürütülen biyojeokimyasal süreçler; lagün ile bitişik kıyı bölgesi arasındaki ilişkiyi anlamak için oldukça önemlidir. Bu çalışma, İzmir Körfezi'nde bulunan ve 67 hektarlık bir alan kaplayan Çakalburnu Lagünü’nde gerçekleştirilmiştir. Çalışmanın amacını, lagün sedimentlerinde, farklı mikrobiyal toplulukların düzeylerinin belirlenmesi oluşturmaktadır. Araştırmada, lagünün 7 noktasından toplanan sediment örneklerinde, Genel Arke (ARC), Metanojenik Arke (MCRA), Anaerobik Metan Oksidasyonu yapan Arke (ANME 1, ANME 2a, ANME 2c), Genel Bakteri (BAC) ve Sülfat İndirgeyen Bakteri (SRB2) seviyelerinin tespitinde Real-Time qPCR analizleri gerçekleştirilmiştir. Çakalburnu Lagünü sedimentlerinde incelenen Genel Arke ve Genel Bakteri bollukların maksimum değerleri sırasıyla 2,66x1010 gen kopya sayısı/gr ve 3,89x107 gen kopya sayısı/gr olarak belirlenmiş olup, bu çalışmada lagün sedimentlerinde arkeal bolluğun yoğun olduğu görülmüştür. Mikrobiyal çeşitliliğin karakterizasyonu, ekosistemin biyolojik temellerinin anlaşılması açısından önemlidir. Çalışmamızda sunulan veriler, lagünler gibi hassas ekosistemlerde, ekolojik ve mikrobiyolojik dengenin korunması ile biyojeokimyasal döngülerin belirlenmesine yönelik çalışmalara katkı sağlamaktadır. Gelecekteki çalışmalarda, mikrobiyal grupların seviyelerinin mevsimlere ve yıllara göre değişimlerinin izlenmesi yönünde çalışmalar yürütülecektir.
KW  - Lagün
KW  - Sediment
KW  - Bakteri
KW  - Arke
KW  - Real-time PCR
KW  - Real-time PCR
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UR  - https://doi.org/10.12714/egejfas.38.2.02
L1  - https://dergipark.org.tr/en/download/article-file/1290149
ER  -