TY  - JOUR
T1  - INVESTlGATlONS ON IN VlTRO FERTlLlZATlON OF RABBIT OVA
AU  - Tekeli, Tevfik
PY  - 1984
DA  - January
DO  - 10.1501/Vetfak_0000000949
JF  - Ankara Üniversitesi Veteriner Fakültesi Dergisi
JO  - Ankara Univ Vet Fak Derg
PB  - Ankara University
WT  - DergiPark
SN  - 1300-0861
SP  - 0
EP  - 0
VL  - 31
IS  - 02
LA  - en
AB  - In this study, in vitro fertilization of rabbit ova wasinvestigated. For this purpose, 42 females and 6 male rabbits (NewZealand) were used during the research. The experimental animalswere divided into 12 groups. PMSG (150-250 LU) and HCG (75-100LU) were injected for superfolliculation and superovulation. Totally62 follicles and 616 ovulation places (corpus haemorrhagicum) werecounted in different times following administration of gonadotrophins.Two hundredfiftyone (37.02 %) ova were collected by flushing oviductsand ruptured follicles with the defined medium. Collected ova were identi/ied under stereo microscope and estimated as normal and degenerated. One Iıundred fourty five (57.76 %), 102 (40.63 %), and 4(1.59%) of ova were covered with zona pellucida, corona radiata and cumulus oophorus, respectively. Totally 236 normalova were used for invitro fertilization. These normalova were incubated witlı capacitatedspermatozoa in tlıe in vitro fertilization medium under paraffin oif whiclıhad been equilibrated with 5 % COı + 95 % Nı at 37-38 oC in a incubator. Af ter 24-28 hours incubation, the ova were controlled understereo microscope and were classified as fertilized or unfertilizedAccording to the results, tlıe in vitro fertilization was observed in47 (19.91 %) out of 236 ova and 34 of fertifized ova were normal andthe rest (13 ova) were abnormal. One hundred eightynine ova were foul/d as unfertilized and degenerated.
KW  - INVESTlGATlONS
KW  - FERTlLlZATlON
KW  - RABBIT OVA
CR  - Ankara Üniversitesi – Ankara Üniversitesi Veteriner Fakültesi Dergisi
UR  - https://doi.org/10.1501/Vetfak_0000000949
L1  - https://dergipark.org.tr/en/download/article-file/1450206
ER  -