TY - JOUR T1 - Melatonin receptors increase Momordica’s anticancer effects against PC-3 and HT-29 TT - Melatonin reseptörleri PC-3 ve HT-29'a karşı Momordica'nın antikanser etkilerini artırır AU - Taghizadehghalehjoughi, Ali AU - Yeni, Yeşim AU - Genç, Sıdıka AU - Wallace, David R AU - Hacimuftuoglu, Ahmet AU - Çakır, Zeynep PY - 2021 DA - March Y2 - 2021 DO - 10.16899/jcm.849993 JF - Journal of Contemporary Medicine JO - J Contemp Med PB - Rabia YILMAZ WT - DergiPark SN - 2667-7180 SP - 166 EP - 173 VL - 11 IS - 2 LA - en AB - Aim: The aim of our study is to the evaluation of melatonin (MLT) and Momordica charantia (MC) combination on PC-3 and HT-29 cancer lines and to address the question of where or not MLT increases MC antitumor effect in the PC-3 and HT-29 cancer lines. Material and Method: The PC-3 and HT-29 cell lines were grown in a manufacturer-specified culture medium. Cisplatin, MLT, increasing concentrations of MC, 40 μg/ml MLT + increasing concentrations MC were applied to PC-3 and HT-29 cell lines for 72 hours. 3-(4,5-Dimethylthiazol-2-Yl)-2,5-Diphenyltetrazolium Bromide (MTT) cell viability, Total Antioxidant Capacity (TAC), Total Oxidant Status (TOS), Cellular Migration (Wound Healing test), and Lactate Dehydrogenase (LDH) tests were done 72 hours after drug administration.Results: The combination of MLT 40 μg/ml + MC 100 µg/ml reduced cell viability in both PC-3 and HT-29 cells. Besides, TAC and TOS levels showed a correlation with LDH and MTT assays and were found to be statistically significant (P KW - HT-29 KW - LDH KW - Melatonin KW - Momordica KW - PC-3 N2 - Amaç: Çalışmamızın amacı, PC-3 ve HT-29 kanser hatlarında melatonin (MLT) ve Momordica charantia (MC) kombinasyonunun değerlendirilmesi ve MLT'nin PC-3 ve HT-29 kanser hatlarında MC antitümör etkisini nerede artırdığı sorusunu ele almaktır. Gereç ve yöntem: PC-3 ve HT-29 hücre çizgileri, üreticinin belirlediği bir kültür ortamında büyütüldü. Sisplatin, MLT, artan MC konsantrasyonları, MLT 40 μg / ml + artan konsantrasyonlar MC, PC-3 ve HT-29 hücre hatlarına 72 saat süreyle uygulandı. 3- (4,5-Dimetiltiyazol-2-Yl) -2,5-Difeniltetrazolyum Bromür (MTT) hücre canlılığı, Toplam Antioksidan Kapasitesi (TAC), Toplam Oksidan Durumu (TOS), Hücresel Göç (Yara İyileştirme testi) ve Laktat Dehidrojenaz (LDH) testleri, ilaç uygulamasından 72 saat sonra yapıldı.Bulgular: MLT 40 ug / ml + MC 100 ug / ml kombinasyonu, hem PC-3 hem de HT-29 hücrelerinde hücre canlılığını azalttı. Ayrıca TAC ve TOS seviyeleri LDH ve MTT testleri ile korelasyon gösterdi ve istatistiksel olarak anlamlı bulundu (P CR - 1. Jemal, A., Global Cancer Statistics (vol 61, pg 69, 2011). Ca-a Cancer Journal for Clinicians, 2011. 61(2): p. 134-134. CR - 2. Knudsen, B.S. and V. Vasioukhin, Mechanisms of Prostate Cancer Initiation and Progression. Advances in Cancer Research, Vol 109, 2010. 109: p. 1-50. CR - 3. Sandhu, G.S. and G.L. Andriole, Overdiagnosis of prostate cancer. J Natl Cancer Inst Monogr, 2012. 2012(45): p. 146-51. CR - 4. Slattery, M.L., Diet, lifestyle, and colon cancer. Semin Gastrointest Dis, 2000. 11(3): p. 142-6. CR - 5. Torre, L.A., et al., Global cancer statistics, 2012. CA Cancer J Clin, 2015. 65(2): p. 87-108. CR - 6. Aklilu, M. and C. Eng, The current landscape of locally advanced rectal cancer. Nat Rev Clin Oncol, 2011. 8(11): p. 649-59. CR - 7. Hanahan, D. and R.A. Weinberg, Hallmarks of cancer: the next generation. Cell, 2011. 144(5): p. 646-74. CR - 8. Braun-Falco, M., et al., [Follicular drug reaction from cetuximab: a common side effect in the treatment of metastatic colon carcinoma]. Hautarzt, 2006. 57(8): p. 701-4. CR - 9. Jia, S., et al., Recent Advances in Momordica charantia: Functional Components and Biological Activities. Int J Mol Sci, 2017. 18(12). CR - 10. Raza, H., et al., Modulation of xenobiotic metabolism and oxidative stress in chronic streptozotocin-induced diabetic rats fed with Momordica charantia fruit extract. J Biochem Mol Toxicol, 2000. 14(3): p. 131-9. CR - 11. Grover, J.K. and S.P. Yadav, Pharmacological actions and potential uses of Momordica charantia: a review. J Ethnopharmacol, 2004. 93(1): p. 123-32. CR - 12. Yodkeeree, S., S. Garbisa, and P. Limtrakul, Tetrahydrocurcumin inhibits HT1080 cell migration and invasion via downregulation of MMPs and uPA. Acta Pharmacol Sin, 2008. 29(7): p. 853-60. CR - 13. de Almeida Chuffa, L.G., et al., Mitochondrial functions and melatonin: a tour of the reproductive cancers. Cell Mol Life Sci, 2019. 76(5): p. 837-863. CR - 14. Calastretti, A., et al., Melatonin Analogue Antiproliferative and Cytotoxic Effects on Human Prostate Cancer Cells. Int J Mol Sci, 2018. 19(5). CR - 15. Eck-Enriquez, K., et al., Pathways through which a regimen of melatonin and retinoic acid induces apoptosis in MCF-7 human breast cancer cells. Breast Cancer Research and Treatment, 2000. 61(3): p. 229-239. CR - 16. Winczyk, K., M. Pawlikowski, and M. Karasek, Melatonin and RZR/ROR receptor ligand CGP 52608 induce apoptosis in the murine colonic cancer. Journal of Pineal Research, 2001. 31(2): p. 179-182. CR - 17. Toubi, E. and Y. Shoenfeld, Protective autoimmunity in cancer (Review). Oncology Reports, 2007. 17(1): p. 245-251. 18. Emsen, B., et al., The anti-cancer efficacies of diffractaic, lobaric, and usnic acid: In vitro inhibition of glioma. Journal of Cancer Research and Therapeutics, 2018. 14(5): p. 941-951. CR - 19. Yeni, Y., et al., Antitumor Effect of Combination Momordica and Melatonin on Prostate Cancer Pc-3 Cell Line. Acta Physiologica, 2019. 227: p. 71-71. CR - 20. Daneshmandi, S., B. Wegiel, and P. Seth, Blockade of Lactate Dehydrogenase-A (LDH-A) Improves Efficacy of Anti-Programmed Cell Death-1 (PD-1) Therapy in Melanoma. Cancers, 2019. 11(4). CR - 21. Ehrke, M.J., Immunomodulation in cancer therapeutics. International Immunopharmacology, 2003. 3(8): p. 1105-1119. CR - 22. Rodriguez-Garcia, A., et al., Thioredoxin 1 modulates apoptosis induced by bioactive compounds in prostate cancer cells. Redox Biology, 2017. 12: p. 634-647. CR - 23. Kanmani, P., et al., Production and purification of a novel exopolysaccharide from lactic acid bacterium Streptococcus phocae PI80 and its functional characteristics activity in vitro. Bioresource Technology, 2011. 102(7): p. 4827-4833. CR - 24. Reiter, R.J., et al., Melatonin, a Full Service Anti-Cancer Agent: Inhibition of Initiation, Progression and Metastasis. International Journal of Molecular Sciences, 2017. 18(4). CR - 25. Beckerandre, M., et al., Pineal-Gland Hormone Melatonin Binds and Activates an Orphan of the Nuclear Receptor Superfamily. Journal of Biological Chemistry, 1994. 269(46): p. 28531-28534. CR - 26. Garcia-Navarro, A., et al., Cellular mechanisms involved in the melatonin inhibition of HT-29 human colon cancer cell proliferation in culture. Journal of Pineal Research, 2007. 43(2): p. 195-205. CR - 27. Papazisis, K.T., et al., Effects of melatonin on proliferation of cancer cell lines. Journal of Pineal Research, 1998. 25(4): p. 211-218. CR - 28. Pariente, R., et al., Participation of MT3 melatonin receptors in the synergistic effect of melatonin on cytotoxic and apoptotic actions evoked by chemotherapeutics. Cancer Chemotherapy and Pharmacology, 2017. 80(5): p. 985-998. CR - 29. Farriol, M., et al., In vitro effects of melatonin on cell proliferation in a colon adenocarcinoma line. Journal of Applied Toxicology, 2000. 20(1): p. 21-24. CR - 30. Pitchakarn, P., et al., Momordica charantia leaf extract suppresses rat prostate cancer progression in vitro and in vivo. Cancer Science, 2010. 101(10): p. 2234-2240. CR - 31. Dia, V.P. and H.B. Krishnan, BG-4, a novel anticancer peptide from bitter gourd (Momordica charantia), promotes apoptosis in human colon cancer cells. Scientific Reports, 2016. 6. CR - 32. Popovich, D.G., et al., Momordica charantia Seed Extract Reduces Pre-Adipocyte Viability, Affects Lactate Dehydrogenase Release, and Lipid Accumulation in 3T3-L1 Cells. Journal of Medicinal Food, 2011. 14(3): p. 201-208. CR - 33. Liu, J., et al., Bog Bilberry (Vaccinium uliginosum L.) Extract Reduces Cultured Hep-G2, Caco-2, and 3T3-L1 Cell Viability, Affects Cell Cycle Progression, and Has Variable Effects on Membrane Permeability. Journal of Food Science, 2010. 75(3): p. H103-H107. UR - https://doi.org/10.16899/jcm.849993 L1 - https://dergipark.org.tr/en/download/article-file/1477537 ER -