@article{article_896489, title={cis-2 and trans-2-eicosenoic Fatty Acids Inhibit Mycobacterium tuberculosis Virulence Factor Protein Tyrosine Phosphatase B}, journal={Journal of the Turkish Chemical Society Section A: Chemistry}, volume={8}, pages={873–882}, year={2021}, DOI={10.18596/jotcsa.896489}, author={Savalas, Lalu Rudyat Telly and Lestari, Asih and Munirah, Munirah and Farida, Suryawati and Suhendra, Dedy and Asnawati, Dina and ’ardhuha, Jannatin and Sarı Ningsih, Baiq and Syahri, Jufrizal}, keywords={eicosenoic fatty acid, Latent tuberculosis infection, Protein tyrosine phosphatase B, molecular docking}, abstract={<p>The present study aims to investigate the potential inhibitory effect of eicosenoic fatty acids on protein tyrosine phosphatase B of Mycobacterium tuberculosis (PtpB). PtpB is recognized to play a vital role in Mycobacterium tuberculosis (Mtb) successful latent infection. It prevents the fusion even between phagocytosed mycobacteria with lysosomes so that the bacteria escape from degradation. We have over-expressed recombinant Mtb PtpB within Escherichia coli BL21(DE3), and further, we have used the protein for inhibition assay with cis-2 and trans-eicosenoic fatty acids. It is revealed that at a concentration of 16 µM, cis-2- and trans-2-eicosenoic fatty acids can inhibit PtpB by 63.72% and 74.67%, respectively. Docking analysis has confirmed strong interactions of PtpB with cis-2 and trans-2-eicosenoic fatty acids, with the binding energy of -60.40 and -61.60 kcal/mol, respectively. These findings underline both fatty acids’ high potential to be further investigated to discover drugs against latent tuberculosis infection. <br /> </p>}, number={3}, publisher={Turkish Chemical Society}, organization={Ministry of Research, Technology, and Higher Education, Republic of Indonesia}