TY  - JOUR
T1  - Boron mine ponds: metagenomic insight to bacterial diversity
TT  - Bor maden göletleri: bakteriyel çeşitliliğe metagenomik bakış
AU  - Nural Yaman, Belma
AU  - Aytar Çelik, Pınar
AU  - Mutlu, Mehmet Burçin
AU  - Korkmaz, Ferhan
AU  - Gedikli, Serap
AU  - Çabuk, Doç. Dr. Ahmet
PY  - 2021
DA  - August
Y2  - 2021
DO  - 10.46309/biodicon.2021.902221
JF  - Biological Diversity and Conservation
JO  - BioDiCon
PB  - Ersin YÜCEL
WT  - DergiPark
SN  - 1308-5301
SP  - 229
EP  - 235
VL  - 14
IS  - 2
LA  - en
AB  - Since it can be a source of new microorganisms with biological potential, it is important to identify the microorganisms found in environments with high boron content in terms of ecological and biotechnological application potentials. In this context, deposit waters in environments where boron mining activities perform are important habitats for boronophilic/boronotolerant microorganisms.In this study, bacterial community in the waste mining ponds of Balıkesir-Bigadiç and Eskişehir-Kırka Boron Mining Operations were investigated by using 16S rDNA gene-targeted Illumina MiSeq sequencing. The greater parts of high-throughput sequences were related to Proteobacteria, Planctomycetes, Bacteriodetes, Verrucomicrobia and Actinobacteria phyla. Cyanobacteria and Parcubacteria were other notable groups with low abundance. Genera belonged to Blastopirellula, Luteolibacter, Porhyrobacter and Hydrogenophaga were the most abundant taxa for all the samples. Sandarakinorhabdus, Pseudoanabena, Roseinatronobacter and Pontimonas genera-affiliated reads were also detected at in the October samples. Striking seasonal variations were detected between samples in terms of the type and number of microbial populations.
KW  - boron
KW  - high throughput sequencing
KW  - microbial diversity
N2  - Biyolojik potansiyeli olan yeni mikroorganizmaların kaynağı olabileceği için, bor içeriği yüksek ortamlarda bulunan mikroorganizmaların belirlenmesi ekolojik ve biyoteknolojik uygulama potansiyelleri açısından önemlidir. Bu bağlamda, bor madenciliği faaliyetlerinin gerçekleştirildiği çevrelerdeki birikinti suları boronofilik / boronotolerant mikroorganizmalar için önemli habitatlardır. Bu çalışmada, Balıkesir-Bigadiç ve Eskişehir-Kırka Bor Maden İşletmeleri atık madenciliği havuzlarındaki bakteri topluluğu, 16S rDNA gen hedefli Illumina MiSeq dizilimi kullanılarak incelenmiştir. Yüksek verimli dizilerin büyük kısımları Proteobacteria, Planctomycetes, Bacteriodetes, Verrucomicrobia ve Actinobacteria filumları ile ilişkili bulunmuştur. Cyanobacteria ve Parcubacteria, düşük miktara sahip diğer önemli gruplar arasındadır. Blastopirellula, Luteolibacter, Porhyrobacter ve Hydrogenophaga'ya ait cinsler tüm örneklerde en çok bulunan taksonlardır. Ekim örneklerinde Sandarakinorhabdus, Pseudoanabena, Roseinatronobacter ve Pontimonas cinsine bağlı okumalar da tespit edilmiştir. Mikrobiyal popülasyonların türü ve sayısı açısından örnekler arasında çarpıcı mevsimsel farklılıklar tespit edilmiştir.
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UR  - https://doi.org/10.46309/biodicon.2021.902221
L1  - https://dergipark.org.tr/en/download/article-file/1658735
ER  -