TY - JOUR T1 - Adsorption of Bovine Serum Albumin BSA on Clinoptilolite AU - Akgül, Murat AU - Savak, Neşe Burcu AU - Özmak, Meryem AU - Dumanlı, Ahu Gümrah AU - Yürüm, Yuda AU - Karabakan, Abdülkerim PY - 2008 DA - January JF - Hacettepe Journal of Biology and Chemistry JO - HJBC PB - Hacettepe University WT - DergiPark SN - 2687-475X SP - 21 EP - 29 VL - 36 IS - 1 LA - en AB - The purpose of the present work was to investigate the adsorption of BSA on clinoptilolite in aqueous media. In thisstudy, batch adsorption experiments were carried out and the effects of pH, protein concentration, ionic strength andmodification of zeolite on the adsorption process were examined. The optimum pH for adsorption was found to be4.0. It was found that various treatments applied to modify clinoptilolite has a substantial effect on the adsorption. Inadsorption studies, residual BSA concentration reached equilibrium in a short duration of 60 min. Maximumadsorption capacity value for clinoptilolite in the original form is 388.3 mg BSA/g zeolite 23.2 mg BSA/m2 and in themodified form ranges from 500-660 mg BSA/g zeolite 25-33 mg BSA/m2 showed that this adsorbent, clinoptilolite,was suitable for the adsorption of BSA from aqueous media KW - Clinoptilolite KW - bovine serum albumin KW - Adsorption CR - 1. Kondo, A., Oku, S., Higashitani, K., J. Colloid, Interface Sci.143 (1991) 214. CR - 2. Haynes, C.A., Norde, W., Colloids Surf. B, Biointerfaces 2 (1994) 517. CR - 3. Mizukami, F., Izutsu, H., T. Osaka, T., Akiyama, Y., Uiji, N., Moriya, K., Endo, K., Maeda, K., Kiyozumi, Y., Sakaguchi, K., J. Chromatogr, A 697 (1995) 279. CR - 4. Akiyama, Y., Mizukami, F., Kiyozumi, Y. , Maeda, K., Izutsu, H., Sakaguchi, K., Angew. Chem. Int. Ed. 38 (1999)1420. CR - 5. Kelleher, B.P., Doyle, A.M., O’Dwyer, T.F., Hodnett, B.K., J.Chem. Technol.Biotechnol, 76 (2001) 1216. CR - 6. Breck, D.W., Zeolite Molecular Sieves, Wiley, London 1974. CR - 7. Szostak, R., van Bekkum, H., Flanigen, E.M., Jansen, J.C., (Eds.), Modified Zeolites, Introduction to Zeolite Science and Practice, Studies in Surface Science and Catalysis, vol. 58, Elsevier, Amsterdam, 1991 (Chapter5). CR - 8. Katiyar, A., Ji, L., Smirniotis, P.G., Pinto, N.G., Microporous and Mesoporous Materials, 80 (2005) 311. CR - 9. Matsui, M., Kiyozumi, Y., Yamamoto, T., Mizushina, Y., Mizukami, F., Sakaguchi, K., Chem. Eur. J., 7 (2001) 1555. CR - 10. Kondo, A., Oku, S., Murakami, F., Higashitani, K., Colloids Surf. B Biointerfaces, 1 (1993) 197. CR - 11. Carter, D.C., and Ho, J.X., Adv. Protein Chem, 45(1994) 153. CR - 12. Figge, J., Rossing, T.H., Fencl, V., J.Lab. Clin. Med, 117 (1991) 453. CR - 13. Coradin, T., Coupe, A., Livage, J., Colloids and Surfaces B: Biointerfaces, 29 (2003) 189. CR - 14. Tarasevich, Y.I., Theor. Exp. Chem, 37 (2001) 98. CR - 15. Giacomelli, C.E., Norde, W., J. Colloid Interface Sci, 233 (2001) 234. CR - 16. Buijs, J., Norde, W., Lichtenbelt, J.W.T., Langmuir, 12 (1996) 1605. CR - 17. Tsu, J., Lu, J.R., Thomas, R.K., Cui, Z.F., Penfold, J., Langmuir, 14 (1998) 438. CR - 18. Ji, L., Katiyar, A., Pinto, N.G., Jaroniec, M. and Smirniotis, P.G., Micropor. And Mesopor. Mater, 75 (2004) 221. CR - 19. Norde, W., Favier, J.P., Colloids Surf, 64 (1992) 87. CR - 20. Jeon, J.S., Raghavan, S., Sperline, R.P., Colloids Surf. A Physicochem. Eng. Aspects, 92 (1994) 255. CR - 21. McClellan, SJ., Franses, E.I., Colloids Surf. A Physicochem. Eng. Aspects, 260 (2005) 265-8. CR - 22. Chiku, H., Matsui, M., Murakami, S., Kiyozumi, Y., Mizukami, F., Sakaguchi, K., Analytical Biochemistry, 318 (2003) 80. CR - 23. Cleland, J. L., Hedgepeth, C., Wang, D.L., J. Biol. Chem, 267 (1992) 13327. CR - 24. Cleland, J. L., Builder, S. E., Swartz, J. R., Winkler, M., Chang, J. Y., Wang, D. I., Biotechnology (N. Y.), 10 (1992) 1013. CR - 25. Molnar, A., Beregszaszi, T., Fudala, A., Lentz, P., Nagy, J.B., Konya, Z., Kiricsi, I., Catal. J., 202 (2001) 379. CR - 26. He, J., Li, X., Evans, D.G., Duan, X., Li, C., J. Mol.Catal. B. Enzym, 11 (2000) 45. CR - 27. Buijs, J., Hlady, V., J. Colloid Interface Sci, 190 (1997) 171. CR - 28. Larsericsdotter, H., Oscarsson, S., Buijs, J., J.Colloid Interface Sci, 237 (2001) 98. CR - 29. Vis, H., Heinemann, U., Dobson, C.M., Robinson, C.V., J. Am.Chem. Soc, 120 (1998) 6427 UR - https://dergipark.org.tr/en/pub/hjbc/article/925892 L1 - https://dergipark.org.tr/en/download/article-file/1727500 ER -