@article{article_926202, title={PEG/Gelatin Composite Hydrogels as a Support of Enzyme Immobilization}, journal={Hacettepe Journal of Biology and Chemistry}, volume={42}, pages={343–350}, year={2014}, author={Ayhan, Fatma and Gülsu, Aydan and Ayhan, Hakan}, keywords={PEG,Gelatin,Photopolymerization,Composite,Enzyme immobilization,Activity}, abstract={In the recent years composite hydrogels have gained considerable interest as a biomaterial vehicle for biomedi- cal applications. The purpose of this study is to prepare highly biocompatible Polyethylene glycol PEG /Gelatin composite hydrogels for Glucose Oxidase GOD immobilization and evaluate the enzyme activity. PEG/Gelatin composite hydrogels were prapered by one step simultaneous technique based on UV-initiated free radical pho- topolymerization. PEG/Gelatin composite hydrogels were prepared by mixing Polyethylene glycol diacrylate PEG 30% - 50% and Gelatin 22.000, 24.000, 40.000, 50.000, 87.500 MW in the precense of ethylene glycol di- methacrylate EGDMA as crosslinker and 2,2-dimethoxy-2-phenylacetophenone DMPA as photo initiator and also GOD 0.1 mg/ml for enzyme immobilization. Enzyme activity studies revealed that GOD immobilized PEG/Gelatin composite hydrogels that contain 30% PEG-DA presented a better activity, lower Km and higher Vmax than GOD immobilized composite hydogels that contain 50% PEG-DA. According to these results composite hydrogels are thought to be adapted for different enzyme systems with the selection of the suitable ratio.}, number={3}, publisher={Hacettepe University}