@article{article_926321, title={Preparation of Hydrophobic Monolithic Columns for On-line Solid-phase Extraction Coupled with HPLC for the Determination of Trace Level Benzo[a]pyrene}, journal={Hacettepe Journal of Biology and Chemistry}, volume={45}, pages={143–151}, year={2017}, url={https://izlik.org/JA47RM55BN}, author={Çorman, Mehmet Emin}, keywords={monolithic column,HPLC,High performance liquid chromatography,solid-phase extraction,benzo[a]pyrene}, abstract={Extraction of trace level persistent organic pollutants is an important issue in water samples. So in this work, p HEMAPA monolithic columns were developed as a novel kind of solid-phase extraction adsorbents, as well as p HEMAPA solid-phase extraction SPE adsorbent was coupled with high-performance liquid chromatography HPLC p HEMAPA-HPLC for the determination of benzo[a]pyrene BaP on-line mode. After p HEMAPA-HPLC para- meters including the injection volume, flow rate, and the sample volume had been optimized in detail, BaP separation procedures on the p HEMAPA-HPLC containing mobile phase, different amounts of BaP, and reusability were perfor- med. Advantages of this procedure are low quantitation limits 0.05–1.0 ng/mL , short extraction time, low consump- tion of organic solvents, and ease of automation and operation of the whole analysis. The experimental results indi- cated that p HEMAPA-HPLC allowed for one-step BaPs extraction, clean-up and analytical determination under the optimal conditions. The reusability of BaPs from water samples was 99.7% with a negligible capacity decrease after ten adsorption–desorption cycles. The method detection limit MDL , relative standard deviation RSD and recovery were obtained as 0.007 ng/mL, 0.26%, and 97.0%, respectively. It was concluded that p HEMAPA monolithic column coupled with HPLC was an excellent alternative for the routine analysis of BaPs at trace level.}, number={2}