@article{article_951265, title={Phycocyanin Extraction From Frozen and Freeze-Dried Biomass of Pseudanabaena sp. by Using Mild Cell Disruption Methods}, journal={Marine Science and Technology Bulletin}, volume={10}, pages={333–339}, year={2021}, DOI={10.33714/masteb.951265}, url={https://izlik.org/JA23ZR39FT}, author={Kısaoğlan, Berke and Demirel, Zeliha and Conk Dalay, Meltem}, keywords={Cyanobacteria, Extraction, Phycobiliprotein, Phycocyanin, Pseudanabaena sp.}, abstract={<div style="text-align:justify;">Phycocyanin is a precious, natural, blue coloured pigment-protein complex that has commercial value and wide application in cosmetics, food, and pharmaceutical industries. In the present study, we performed various cell disruption methods (ultrasonication, homogenization, freeze/thaw and CaCl2 extraction) for phycocyanin extraction from different forms of biomass of a thermophilic Pseudanabaena sp. that has a high potential to produce high-quality phycocyanin. Using potassium phosphate buffer and ultrasonic bath method, we achieved the highest phycocyanin yield (345 mgPC.g <sup>-biomass </sup>) from freeze-dried biomass and we obtained increased yield as the duration of application increases. Phycocyanin yields were calculated as 345 mgPC.g <sup>-biomass </sup>, 255 mgPC.g <sup>-biomass </sup> and 220 mgPC.g <sup>-biomass </sup> for 5, 10 and 15 min, respectively. In this study, cell disruption methods have determined significantly more effective on freeze-dried biomass rather than frozen biomass. Phycocyanin content of freeze-dried biomass was analysed after six months of storage and dramatic decrement was observed in the phycocyanin content of the cells. </div>}, number={4}