@article{article_963979, title={ISOLATION OF NEWLY ISOLATED VB_K1 BACTERIOPHAGE AND INVESTIGATION OF SUSCEPTIBILITY ON ESBL POSITIVE KLEBSIELLA SPP. STRAINS}, journal={Journal of Faculty of Pharmacy of Ankara University}, volume={45}, pages={515–523}, year={2021}, DOI={10.33483/jfpau.963979}, author={Erol, Hilal Basak and Kaşkatepe, Banu}, keywords={Bacteriophage, ESBL, Klebsiella spp., resistance}, abstract={<div style="text-align:justify;"> <b>Objective: </b> <i>The decrease in the efficacy of antimicrobials in the treatment of Klebsiella-related infections necessitated the search for alternative treatment strategies. This study aims to provide isolation of lytic bacteriophage specific to Klebsiella species and to investigate its potential for use as alternative antimicrobial agent. </i> </div> <div style="text-align:justify;"> <b>Material and Method: </b> <i> One Extended spectrum beta lactamase (ESBL) producer Klebsiella strain was used as host bacteria and water samples were collected from river in Ankara for bacteriophage isolation. Spot test method was applied to determine the possible presence of bacteriophage after phage enrichment. To confirm the presence of the lytic bacteriophage, double layer agar method was applied to spot test positive samples. The susceptibility of the bacteriophage was determined using in vitro spot test. 38 clinical ESBL positive Klebsiella spp. strains were used for this analysis. </i> </div> <div style="text-align:justify;"> <b>Result and Discussion: </b> <i>In the initial screening, the vB_K1 bacteriophage producing visible plaques with a diameter of 1.00 mm was isolated in the petri dish. The susceptibility of ESBL positive Klebsiella spp. strains to this bacteriophage was determined as 73.7%. It was proved that vB_K1 bacteriophage is very effective to Klebsiella spp. strains. However, in vitro bacteriophage susceptibility of characterized bacteriophage is encouraging development. </i> </div>}, number={3}, publisher={Ankara University}