@article{article_968396, title={Evaluation Of Clonal Relationship Between Acinetobacter baumannii Isolates and Determination Of Resistance To Antibiotics Which Were Obtained from Different Hospitals In Ankara}, journal={Türk Hijyen ve Deneysel Biyoloji Dergisi}, volume={73}, pages={199–210}, year={2016}, author={Şahin, Hünkar and Önde, Ufuk and Adiloğlu, Ali Kudret and Karakoç, Ayşe Esra and Bulut, Cemal and Açıkgöz, Ziya Cibali and Erdem, Gül Bahar and Hasçelik, Gülşen and Coşkun, Ayşegül Öztürk}, keywords={Acinetobacter baumannii, clonal relationship, rep-PCR, antibiotic resistance}, abstract={Objective: Acinetobacter baumannii is one of the most important cause of hospital acquired infections which raises the rates of mortality and morbidity especially in intensive care units. Acinetobacter baumannii may be isolated from various infections such as pneumonia, bacteremia, urinary track infections, wound infections and meningitis.The aim of this study is to determine the clonal relationship and determination of antibiotic resistances between 99 Acinetobacter baumannii isolates which were obtained from different hospitals in Ankara between November 2009 and December 2011. Method: Identification of A. baumannii isolates and antibiotic susceptibilities were performed by Vitek 2 bioMérieux, France system. In this study, amikacin, ciprofloxacin, tetracyclin, netilmicin, sulbactam/ampicillin, trimethoprim/sulfamethoxazole, ceftazidime, gentamicin, levofloxacin, meropenem, imipenem, piperacillin, piperacillin/tazobactam, cefoperazone/sulbactam, cefepime, tigecycline, and colistin were used as antimicrobial agents. The clonal relationship of Acinetobacter isolates was analysed by Rep-PCR method. Results: Ninety nine percent of A. baumannii isolates was resistant to imipenem and meropenem. There was no resistant strain to colistine 0% . All of the strains were resistant to piperacillin and piperacillin/tazobactam 100% . Ciprofloxacin was 99%, sulbactam/ampicillin 98%, ceftazidime was 97%, cefepime was 96%, levofloxacin was 85%, tetracycline was 80%, trimethoprim/sulfamethoxazole was 71% resistant. Resistance to amikacin was 59% and resistance to gentamicin and netilmicin were both 56%. Five percent of strains were resistant, 27% were intermediate susceptile and 68% were susceptile to tigecycline. There were four patterns. P1, P2 and P3 gathered to one group G1 and they were similar. P4 was different from them. P1 had 14, P2 had 37, P3 had 36 and P4 had 12 strains. There were more than three bands difference between G1 and P4 which showed that they were different strains. Band difference between G1 P1, P2, P3 was only one and this showed us that they were similar. In the other hand, there were not any band diffirence in P1, P2, P3 and P4. All of the patterns were consisted of strains from different hospitals.We determined that the same strains spread to four different hospitals probably by patient transfers. Conclusion: A study can be organized to determine resistance genes and resistance mechanisms of A. baumannii in these hospitals as continuation of this study. Moreover, a comprehensive epidemiologic data can be revealed by determining the risk factors of patients relevant to those responsible isolates for carbapenem and other antibiotics resistance.}, number={3}, publisher={T.C. Sağlık Bakanlığı Halk Sağlığı Genel Müdürlüğü}