TY  - JOUR
T1  - Effects of sodium nitrate on the growth and proximate composition of the indigenous marine microalgae Tetraselmis chuii (Butcher, 1959)
AU  - Khatoon, Helena
AU  - Barman, Sanzib Kumar
AU  - Rahman, Mohammad Redwanur
AU  - Mazumder, Sabuj Kanti
AU  - Hasan, Shanur
PY  - 2022
DA  - January
DO  - 10.26650/ASE2021972678
JF  - Aquatic Sciences and Engineering
JO  - Aqua Sci Eng
PB  - Istanbul University
WT  - DergiPark
SN  - 2602-473X
SP  - 46
EP  - 52
VL  - 37
IS  - 1
LA  - en
AB  - Nitrogen  is  one  of  the  fundamental  nutrients  for  algal  growth,  underpinning  the  microalgal  bio-chemical composition. Therefore, this study compared the growth and proximate compositions of Tetraselmis chuii (Butcher, 1959), cultured in different nitrate (NaNO3) concentrations (25, 50, 100, 200 and 500 mg L-1). Thus, the cell density, optical density, specific growth rate and division rate of T.  chuii  were  measured  daily.  Furthermore,  protein  and  carbohydrate  contents  were  also  deter-mined in the stationary phase. The results showed that T. chuii cultivated in a NaNO3 concentration of  500  mg  L-1  had  significantly  (p<0.05)  higher  growth  in  terms  of  cell  density,  biomass,  optical  density, specific growth rate and division rates compared with other concentrations. Likewise, pro-tein content was also significantly higher under the NaNO3 concentration of 500 mg L-1, whereas significantly (p<0.05) higher carbohydrate content was found at 25 mg L-1 NaNO3 compared with the other concentrations, showing a contrary trend between protein and carbohydrate concentra-tions, respectively. Since the primary focus has been on improving the quality of microalgal bio-mass in order to develop novel processes and products, this is the first study to use higher concen-trations  of  modified  nitrate  on  T.  chuii  isolated  from  the  coastal  area  of  Bangladesh.  Thus  the  indigenous marine T. chuii had significantly utilised NaNO3 concentrations with higher growth and proximate  contents  in  this  study.  However,  further  study  is  needed  on  microalgal  genetics  and  metabolic engineering to create a new molecular era of the indigenous marine microalgae isolated from the coastal water of Bengal.
KW  - Nitrogen concentrations
KW  - marine microalgae
KW  - growth
KW  - proximate composition
CR  - Ahmad, M. T., Shariff, M., Md. Yusoff, F., Goh, Y. M. & Banerjee, S. (2020). Applications of microalga Chlorella vulgaris in aquaculture. Reviews in Aquaculture, 12(1), 328-346. [CrossRef] google scholar
CR  - Araujo, G. S., Silva, J. W., Viana, C. A. & Fernandes, F. A. (2020). Effect of sodium nitrate concentration on biomass and oil production of four microalgae species. International Journal of Sustainable Energy, 39(1), 41-50. [CrossRef] google scholar
CR  - Bartley, M. L., Boeing, W. J., Daniel, D., Dungan, B. N. & Schaub, T. (2016). Optimization of environmental parameters for Nannochloropsis salina growth and lipid content using the response surface method and invading organisms. Journal of applied phycology, 28, 15-24. [CrossRef] google scholar
CR  - Butcher, R. W. (1959). An introductory account of the smaller algae of British coastal waters Part I. Introduction and Chlorophyceae. Fish Investig, 4, 1-74. google scholar
CR  - Cai, T., Park, S. Y. & Li, Y. (2013). Nutrient recovery from wastewater streams by microalgae: status and prospects. Renewable and Sustainable Energy Reviews, 19, 360-369. [CrossRef] google scholar
CR  - Clesceri, L. S., Greenberg, A. E. & Trussel, R. R. (1989). Standard methods for the examination of water and wastewater, Published by the Am. Publ. Health Assoc., Am. Water Works Assoc. and Water Pollution Control Fed. google scholar
CR  - Darvehei, P., Bahri, P. A. & Moheimani, N. R. (2018). Model development for the growth of microalgae: A review. Renewable and Sustainable Energy Reviews, 97, 233-258. [CrossRef] google scholar
CR  - Dubois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. T. & Smith, F. (1956). Colorimetric method for determination of sugars and related substances. Analytical chemistry, 28(3), 350-356. [CrossRef] google scholar
CR  - El-Kassas, H. Y. (2013). Growth and fatty acid profile of the marine microalga Picochlorum sp. grown under nutrient stress conditions. The Egyptian Journal of Aquatic Research, 39(4), 233-239. [CrossRef] google scholar
CR  - Ghezelbash, F., Farboodnia, T., Heidari, R. & Agh, N. (2008). Biochemical effects of different salinities and luminance on green microalgae Tetraselmis chuii. Research Journal of Biological Sciences, 3(2), 217221. google scholar
CR  - Gu, H., Nagle, N., Pienkos, P. T. & Posewitz, M. C. (2015). Nitrogen recycling from fuel-extracted algal biomass: residuals as the sole nitrogen source for culturing Scenedesmus acutus. Bioresource technology, 184, 153-160. [CrossRef] google scholar
CR  - Guiheneuf, F. & Stengel, D. B. (2015). Towards the biorefinery concept: Interaction of light, temperature and nitrogen for optimizing the co-production of high-value compounds in Porphyridium purpureum. Algal research, 10, 152-163. [CrossRef] google scholar
CR  - Hibberd, D. J. (1981). Notes on the taxonomy and nomenclature of the algal classes Eustigmatophyceae and Tribophyceae (synonym Xanthophyceae). Botanical journal of the linnean society. [CrossRef] google scholar
CR  - Ho, S. H., Ye, X., Hasunuma, T., Chang, J. S. & Kondo, A. (2014). Perspectives on engineering strategies for improving biofuel production from microalgae—a critical review. Biotechnology advances, 32(8), 1448-1459. [CrossRef] google scholar
CR  - Huang, X., Huang, Z., Wen, W. & Yan, J. (2013). Effects of nitrogen supplementation of the culture medium on the growth, total lipid content and fatty acid profiles of three microalgae (Tetraselmis subcordiformis, Nannochloropsis oculata and Pavlova viridis). Journal of Applied Phycology. [CrossRef] google scholar
CR  - James, D. B. (1996). Inception report on sea cucumber culture in Laamu Atoll, Maldives. Food and Agriculture Organization of the United Nations, Bangkok, TCP/MDV/4452 Field Document 1. google scholar
CR  - Khatoon, H., Banerjee, S., Yusoff, F. M. & Shariff, M. (2013). Use of microalgal - enriched D iaphanosoma celebensis S tingelin, 1900 for rearing L itopenaeus vannamei (B oone, 1931) postlarvae. Aquaculture Nutrition, 19, 163-171. [CrossRef] google scholar
CR  - Khatoon, H., Haris, H., Rahman, N. A., Zakaria, M. N., Begum, H. & Mian, S. (2018). Growth, proximate composition and pigment production of Tetraselmis chuii cultured with aquaculture wastewater. Journal of Ocean University of China, 17 (3), 641-646. [CrossRef] google scholar
CR  - Khatoon, H., Rahman, N. A., Banerjee, S., Harun, N., Suleiman, S. S., Zakaria, N. H. & Endut, A. (2014). Effects of different salinities and pH on the growth and proximate composition of Nannochloropsis sp. and Tetraselmis sp. isolated from South China Sea cultured under control and natural condition. International Biodeterioration & Biodegradation, 95, 11-18. [CrossRef] google scholar
CR  - Kim, G., Mujtaba, G. & Lee, K. (2016). Effects of nitrogen sources on cell growth and biochemical composition of marine chlorophyte Tetraselmis sp. for lipid production. Algae, 31(3), 257-266. [CrossRef] google scholar
CR  - Lavens, P. & Sorgeloos, P. (1996). Manual on the production and use of live food for aquaculture (No. 361). Food and Agriculture Organization (FAO). google scholar
CR  - Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. (1951). Protein measurement with the Folin phenol reagent. Journal of biological chemistry, 193, 265-275. [CrossRef] google scholar
CR  - Mohsenpour, S. F. & Willoughby, N. (2016). Effect of CO2 aeration on cultivation of microalgae in luminescent photobioreactors. Biomass and Bioenergy, 85, 168-177. [CrossRef] google scholar
CR  - Mostafa, S. S. (2012). Microalgal biotechnology: prospects and applications. Plant science, 12, 276-314. google scholar
CR  - Nigam, P. S. & Singh, A. (2011). Production of liquid biofuels from renewable resources. Progress in energy and combustion science, 37(1), 52-68. [CrossRef] google scholar
CR  - Ördög, V., Stirk, W. A., Bâlint, P., van Staden, J. & Lovâsz, C. (2012). Changes in lipid, protein and pigment concentrations in nitrogen-stressed Chlorella minutissima cultures. Journal of Applied Phycology, 24(4), 907-914. [CrossRef] google scholar
CR  - Pancha, I., Chokshi, K., George, B., Ghosh, T., Paliwal, C., Maurya, R. & Mishra, S. (2014). Nitrogen stress triggered biochemical and morphological changes in the microalgae Scenedesmus sp. CCNM 1077. Bioresource technology, 156, 146-154. [CrossRef] google scholar
CR  - Ramanna, L., Rawat, I. & Bux, F. (2017). Light enhancement strategies improve microalgal biomass productivity. Renewable and Sustainable Energy Reviews, 80, 765-773. [CrossRef] google scholar
CR  - Razaghi, A., Godhe, A. & Albers, E. (2014). Effects of nitrogen on growth and carbohydrate formation in Porphyridium cruentum. Open Life Sciences, 9(2), 156-162. [CrossRef] google scholar
CR  - Rizwan, M., Mujtaba, G., Rashid, N. & Lee, K. (2017). Enhancing lipid production of Dunaliella tertiolecta by manipulating the interactive effect of salinity and nitrogen. Chemical and Biochemical Engineering Quarterly, 31(3), 199-207. [CrossRef] google scholar
CR  - Sathasivam, R., Radhakrishnan, R., Hashem, A. & Abd_Allah, E. F. (2019). Microalgae metabolites: A rich source for food and medicine. Saudi journal of biological sciences, 26(4), 709-722. [CrossRef] google scholar
CR  - Teo, C. L., Atta, M., Bukhari, A., Taisir, M., Yusuf, A. M. & Idris, A. (2014). Enhancing growth and lipid production of marine microalgae for biodiesel production via the use of different LED wavelengths. Bioresource technology, 162, 38-44. [CrossRef] google scholar
CR  - Tseng, C. K., Jiaofen, C. & Zhefu, Z. (1992). On a new species of Pavlova (Prymnesiophyceae) from China. Chinese Journal of Oceanology and Limnology, 10(1), 23-30. [CrossRef] google scholar
CR  - Uggetti, E., Sialve, B., Hamelin, J., Bonnafous, A. & Steyer, J. P. (2018). CO2 addition to increase biomass production and control microalgae species in high rate algal ponds treating wastewater. Journal of CO2 Utilization, 28, 292-298. [CrossRef] google scholar
CR  - Wu, H. & Miao, X. (2014). Biodiesel quality and biochemical changes of microalgae Chlorella pyrenoidosa and Scenedesmus obliquus in response to nitrate levels. Bioresource Technology, 170, 421-427. [CrossRef] google scholar
CR  - Xia, S., Wan, L., Li, A., Sang, M. & Zhang, C. (2013). Effects of nutrients and light intensity on the growth and biochemical composition of a marine microalga Odontella aurita. Chinese Journal of Oceanology and Limnology, 31(6), 1163-1173. [CrossRef] google scholar
CR  - Yeh, K. L. & Chang, J. S. (2012). Effects of cultivation conditions and media composition on cell growth and lipid productivity of indigenous microalga Chlorella vulgaris ESP-31. Bioresource technology, 105, 120-127. [CrossRef] google scholar
CR  - Yusof, N. S., Yeong, Y. S., Zakeri, H. A., Wahid, M. E. A., Ab, N. & Ghafar, N. Y. (2021). Photoperiod influenced the growth and antioxidative responses of Chlorella vulgaris, Isochrysis galbana, and Tetraselmis chuii. J Appl Pharm Sci, 11(4), 125-134. google scholar
CR  - Zarrinmehr, M. J., Farhadian, O., Heyrati, F. P., Keramat, J., Koutra, E., Kornaros, M. & Daneshvar, E. (2020). Effect of nitrogen concentration on the growth rate and biochemical composition of the microalga, Isochrysis galbana. The Egyptian Journal of Aquatic Research, [CrossRef] google scholar
CR  - Zhu, S., Huang, W., Xu, J., Wang, Z., Xu, J. & Yuan, Z. (2014). Metabolic changes of starch and lipid triggered by nitrogen starvation in the microalga Chlorella zofingiensis. Bioresource Technology, 152, 292298. [CrossRef] google scholar
UR  - https://doi.org/10.26650/ASE2021972678
L1  - https://dergipark.org.tr/en/download/article-file/1885393
ER  -