@article{article_975024, title={PHYTOCHEMICAL AND IN VITRO PHARMACOLOGICAL EVALUATION OF PHLOMIS PUNGENS}, journal={Journal of Faculty of Pharmacy of Ankara University}, volume={46}, pages={11–22}, year={2022}, DOI={10.33483/jfpau.975024}, author={Okur, Mehmet Evren and Karadağ, Ayşe Esra and Reis, Rengin and Sipahi, Hande and Arslan, Rana and Demirci, Betül and Demirci, Fatih}, keywords={Cell culture, fibroblast, inflammation, Lamiaceae, macrophage}, abstract={<div style="text-align:justify;"> <b>Objective: </b> <i>This study aimed to investigate the in vitro wound healing, anti-inflammatory, antimicrobial and antioxidant activity of Phlomis pungens Willd. extract derived from the aerial parts. </i> <br /> <b> Material and Method: </b> <i>The phytochemical analysis was performed using GC-MS in order to identify the volatile components of the bioactive Hex extract. The wound healing activity of P. pungens extract was evaluated based on in vitro antimicrobial, antioxidant, anti-inflammatory, and, scratch activity was studied. In addition, the in vitro cytotoxicity of the extract was also evaluated. </i> <br /> <b> Result and Discussion: </b> <i>P. pungens methanol extract depicted a 5-LOX inhibitory activity at 78.2µg/mL (IC50), while the antioxidant activity by DPPH radical provided an IC50=2.41mg/mL, and the ABTS radical showed IC50=3.32mg/mL, respectively. The extract showed dose-dependently anti-inflammatory activity while L-NAME and P. pungens methanol extract significantly decreased LPS stimulated PGE2 production. According to the scratch assay results, all treatments led to an increase in cell migration rate with a dose-dependent effect. Our findings suggested that P. pungens methanol extract may have a role in wound healing according to the scratch test, and it is thought that its antioxidant and anti-inflammatory activity also contributed. Further evaluations are ongoing to confirm the in vitro activity under in vivo conditions. </i> <br /> </div>}, number={1}, publisher={Ankara University}