@article{article_984139, title={Intracytoplasmic Sperm Injection (ICSI) in B6D2F1 and CB6F1 Strains Mice Using Cauda Epididymal Spermatozoa}, journal={Experimed}, volume={11}, pages={179–183}, year={2021}, DOI={10.26650/experimed.2021.984139}, author={Taşkın, Dr. Ali Cihan and Kocabay, Ahmet and Coşkun, Nilhan}, keywords={Mouse, oocyte, ICSI, development}, abstract={<p>Objective: Reproductive biotechnology studies focus on the long-term storage of embryos (cryopreservation), embryo cultures, ge-nome editing of embryos and embryo transfer. Micromanipulation techniques in reproduction biotechnologies have an important role, especially in studies investigating assisted reproductive tech-nology in laboratory animals. The aim of the present study was to investigate the effect of epididymal spermatozoa injected to oo-cyte by intracytoplasmic sperm injection (ICSI) in different mice strains. In this study, we evaluated the in vitro development of post-ICSI derived embryos using cauda epididymal sperm. </p> <p>Material and Method: Female mice (8-10 weeks) were superovulated using pregnant mare serum gonadotropin/human chorionic gonadotropin (PMSG/hCG) and ~14h post hCG, the mice were sacrificed, and the oocytes were collected. Spermatozoa from the cau-da epididymal of a 12-week-old were used on the same strain for ICSI and the in vitro developmental potential was evaluated. Finally, the embryos were cultured for 120 hours at 5% CO2 with 37°C. </p> <p>Results: The results showed that the two-cell embryo of the B6D2F1 strain (79.31%) was significantly higher than the CB6F1 (56.26%) (p<0.05). While the blastocyst rate was comparable between both the B6D2F1 strain (68.75%) and CB6F1 strain (69.57%) (p>0.05). </p> <p>Conclusion: ICSI using cauda epididymal sperm is a suitable ap-plication for in vitro embryo development in B6D2F1 and CB6F1 strains. Finally, ICSI success of the B6D2F1 mice strains was found to be higher than CB6F1 mice strains. </p>}, number={3}, publisher={Istanbul University}, organization={TÜBİTAK}