Sıçanlarda oluşturulan cerrahi müdahalesiz yeni bir deneysel Asherman sendromu modeli

Year 2018, Volume:3 Issue: 3, 148 - 151, 30.11.2018

Başak Büyük

https://doi.org/10.25000/acem.434883

Abstract

Amaç:
Asherman sendromu(AS) uterin kaviteye travma sonucu kavitenin adezyonlarla
kısmi ya da tam tıkanması durumudur. Klinik öncesi çalışmalarla yeni tedavi
yöntemlerinin etkinliğini gösterebilmek için öncelikle AS modelinin hayvanda
oluşturulmasına ihtiyaç vardır. Bu çalışmanın amacı, AS ve intrauterin adezyon
modellemesinde kullanılmak üzere cerrahi uygulanmaksızın yeni ve etkili bir
yöntem geliştirmek, bu şekilde araştırmacılara güvenlik ve uygulanabilirlik
açısından daha etkin bir metot önerebilmektir.

Yöntem:
On iki dişi Wistar Albino sıçan rastgele 2 gruba
ayrıldı. Daha önceden hazırlanmış
olan pembe renk (20 gauge) kateterle transvajinal yoldan hayvanların sol uterin hornularına ulaşıldı.
Grup I’deki hayvanlara 0,2 ml serum fizyolojik uygulandı, Grup II’dekilere ise 0,2 ml Triklorasetik asit (TCA) uygulandı. Üç menstruel siklus beklendikten
sonra hayvanların uterusları çıkarılıp Hematoksilen-Eozin ve Masson Trikrom boyamalar
yapılarak histopatolojik olarak değerlendirildi. Yapılan Hematoksilen-Eozin
boyalı kesitlerde inflamasyon dereceleri değerlendirilirken, Masson Trikrom
boyalı kesitlerde fibrozis dereceleri değerlendirildi.

Bulgular: Grup 1’e
ait uterus kesitleri normal histolojik görünüme sahipken, Grup 2’ye ait sol
uterus kesitlerinde inflamasyon ve fibrozis oluştuğu gözlendi. Bu sonuçlar
istatistiksel olarak anlamlıydı (p=0,002).

Sonuç: Sonuç
olarak, önerdiğimiz cerrahi müdahalesiz AS modelleme yöntemi, literatürde
önerilen yöntemler ile benzer şekilde hastalık oluşturmuş ve bu etki, yapılan
histopatolojik incelemelerle de ortaya konmuştur. Bu şekilde, AS için doğru bir
hastalık modellemesi gerçekleştiren cerrahisiz yeni bir yöntem önerilmiştir.

Keywords

Asherman sendromu, sıçan, jinatrezi, patoloji, hayvan modeli

A new nonsurgical experimental model for Asherman syndrome created in rats

Abstract

Aim: Asherman Syndrome (AS) is a partial or complete obstruction
of the uterine cavity with adhesions as a result of trauma. In pre-clinical
studies, to be able to show the effectiveness of new treatment methods, first
of all, the AS model needs to be created in the animals. The aim of this study
is to develop a new and effective nonsurgical method for using in AS and intrauterine
adhesions modeling, and through this way, to propose a more effective method
for researchers in terms of safety and feasibility.

Methods: Twelve
female Wistar Albino rats were divided into two groups. It was reached to the left uterine horn transvaginally
by using pre-prepared pink color (20 gauge) cannula. While 0.2 ml normal saline
was applied to the animals in Group I (control group), 0.2 ml (Trichloroacetic
acid) TCA was applied to the animals in Group II (experiment group).The right
uterine horns of the animals were left without treatment. After three menstrual
cycles, the animals were sacrificed and Hematoxylin-Eosin and Masson’s Trichrom
staining were performed and evaluated histopathologically. Inflammation was
evaluated in Hematoxylin-Eosin staining and fibrosis was evaluated in Masson’s
Trichrom staining.

Results: Whereas the
uterine sections of the Group I have normal histologic appearance, inflammation
and fibrosis were found in the left uterine sections of the Group 2 by
histopathological evaluation. Results were statistically significant (p=0.002).

Conclusion: The
proposed nonsurgical AS modeling method created disease, and this was also
revealed by histopathological examinations. Through this way, a new AS model is suggested
without surgery, in which the disease is correctly created.

Keywords

Asherman Syndrome, rat, gynatresia, pathology, animal model

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