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Identification and virules of Sphaeropsis tip blight (Sphaeropsis sapinea) on Pinus spp. in Istanbul and Bursa parks

Year 2018, Volume: 31 Issue: 1, 17 - 21, 30.03.2018

Abstract

Sphaeropsis sapinea tip blight is one of
the most destructive fungal diseases of pine trees in the world.
It causes shoot blight, canker, collar rot, root disease
and blue stain of Pinus spp.
As a result of surveys
performed in the parks, recreation areas and groves in Istanbul and Bursa
provinces in January, June and October 2015, it was observed that the agent
especially causes disease on Pinus
spp.
The
disease was observed as dead, brown needles at the tips of branches, shoot
blight and cankers on pine stems and branches.
Twenty eight diseased pine samples were collected from parks, recreation
areas and groves.
Totally
15
Sphaeropsis sapinea isolates were
obtained.
Cultural and
morphological characteristics of the isolates were determined using cultures
grown on potato dextrose agar (PDA).
Cultures on PDA were initially white
with profuse aerial mycelia that after one week turned grey to blackish.
Identification
of the pathogen was based on morphological characteristics of the conidia and
DNA sequence analysis. Genomic DNA was
extracted and the internal transcribed spacer (ITS) region of the rDNA was
amplified using primers ITS1 and ITS 4. Pathogenicity tests were made with twig
inoculation method. All isolates formed lesions on twigs.

References

  • Aroca A., Raposo R. 2007. PCR-based stragety to detect and identify species of Phaeoacremonium causing grapevine diseases. Applied and Environmental Microbiology, 73: 2911-2918. Brookhouser L.W., Peterson G.W. 1971. Infection of Austrian, Scots and Ponderosa pines by Diplodia pinea. Phytopathology, 6: 439-444. Chou C.K.S. 1976. A shoot dieback in Pinus radiata caused by Diplodia pinea II. Inoculation studies. New Zealand Journal of Forestry Science, 6: 409-420. Chou C.K.S., MacKenzie M. 1988. Effect of pruning intensity and season on Diplodia pinea infection of Pinus radiata stems through pruning wounds. European Journal of Forest Pathology, 18: 437-444. Chou C.K.S. 1987. Crown wilt of Pinus radiata associated with Diplodia pinea infection of woody stems. European Journal of Forest Pathology, 17: 398-411. Cobos R., Martin M.T. 2008. Molecular characterization of Phaeomoniella chlamydospora isolated from grapevines in Castilla y León (Spain). Phytopathologia Mediterranea, 47: 20-27. De Wet J., Wingfield M. J., Coutinho T.A., Wingfield B.D. 2000. Characterization of Sphaeropsis sapinea isolates from South Africa, Mexico, and Indonesia. Plant Disease, 84: 151-156. Doğmuş-Lehtijärvi H.T., Kaya A.G.A., Lehtijärvi A., Oskay F., Kaya Ö.D. 2014. Occurrence and genetic similarity of Diplodia pinea on shoots and cones in seed orchards of Pinus spp. in north-western Turkey. Plant Protection Science, 50: 217-220. Doğmuş-Lehtijärvi H.T., Lehtijärvi A., Karaca G., Aday A.G. 2007. Sphaeropsis sapinea Dyko & Sutton associated with shoot blight on Brutian pine in southwestern Turkey. Acta Silvatica & Lignaria Hungarica, Special Edition 2007: 95-99. Kaya A.G.A., Lehtijärvi A., Kaya Ö.D., Doğmuş-Lehtijärvi H.T. 2014. First report of Diplodia pinea on Pseudotsuga menziesii in Turkey. Plant Disease, 98: 689. Nicholls T.H., Ostry M.E. 1990. Sphaeropsis sapinea cankers on stressed red and jack pines in Minnesota and Wisconsin. Plant Disease, 74: 54-56. Palmer M.A., Nicholls T.H. 1985. Shoot blight and col¬lar rot of Pinus resinosa caused by Sphaeropsis sapinea in forest tree nurseries. Plant Disease, 69: 739-740. Peterson G.W. 1977. Infection, epidemiology and control of Diplodia blight of Austrian, Ponderosa and Scots pines. Phytopathology, 67: 511-514. Peterson G.W. 1981. Diplodia Blight of Pines, Forest Insect& Disease Leaflet 161, U.S. Agriculture Forest Service. www.na.fs.fed.us/spfo/pubs/fidls/diplodia/diplodiafidl.htm Rees A.A., Webber J.F. 1988. Pathogenicity of Sphaeropsis sapinea to seed, seedlings and saplings of some Central American pines. Transactions of the British Mycological Society, 91: 273-277. Sinclair W.A., Lyon H.H. 1987. Disease of Trees and Shrubs. Department of Plant Pathology. Cornell Univer¬sity Press, Ithaca, London, 136-137. Stanosz G.R., Blodgett J.T., Smith D.R., Kruger E.L. 2001. Water stress and Sphaeropsis as a latent pathogen of red pine seedlings. New Phytologist, 149: 531-538. Stanosz G.R., Cummings-Carlson J. 1996. Association of mortality of recently planted seedlings and established saplings in red pine plantations with Sphaeropsis collar rot. Plant Disease, 80: 750-753. Sümer S. 2000. Orman Bakanlığı Makamına Kahramanmaraş Yöresi için Hazırlanan Rapor, İstanbul. Swart W.J., Wingfield M.J., Knox-Davies P.S. 1987. Conidial dispersal of Sphaeropsis sapinea in three climat¬ic regions of South Africa. Plant Disease, 71: 1038-1040. White T.J., Bruns T., Lee S., Taylor J.W., 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: M.A. Innis, D.H. Gelfand, J.J. Sninsky White T.J. (Eds.). PCR Protocols: A Guide to Methods and Applications. Academic Press, Inc., New York. pp: 315-322. Wingfield M.J., Know-Davies P.S. 1980. Observation on diseases in pine and eucalyptus plantations in South Africa. Phytophylactica, 12: 57-63.
Year 2018, Volume: 31 Issue: 1, 17 - 21, 30.03.2018

Abstract

References

  • Aroca A., Raposo R. 2007. PCR-based stragety to detect and identify species of Phaeoacremonium causing grapevine diseases. Applied and Environmental Microbiology, 73: 2911-2918. Brookhouser L.W., Peterson G.W. 1971. Infection of Austrian, Scots and Ponderosa pines by Diplodia pinea. Phytopathology, 6: 439-444. Chou C.K.S. 1976. A shoot dieback in Pinus radiata caused by Diplodia pinea II. Inoculation studies. New Zealand Journal of Forestry Science, 6: 409-420. Chou C.K.S., MacKenzie M. 1988. Effect of pruning intensity and season on Diplodia pinea infection of Pinus radiata stems through pruning wounds. European Journal of Forest Pathology, 18: 437-444. Chou C.K.S. 1987. Crown wilt of Pinus radiata associated with Diplodia pinea infection of woody stems. European Journal of Forest Pathology, 17: 398-411. Cobos R., Martin M.T. 2008. Molecular characterization of Phaeomoniella chlamydospora isolated from grapevines in Castilla y León (Spain). Phytopathologia Mediterranea, 47: 20-27. De Wet J., Wingfield M. J., Coutinho T.A., Wingfield B.D. 2000. Characterization of Sphaeropsis sapinea isolates from South Africa, Mexico, and Indonesia. Plant Disease, 84: 151-156. Doğmuş-Lehtijärvi H.T., Kaya A.G.A., Lehtijärvi A., Oskay F., Kaya Ö.D. 2014. Occurrence and genetic similarity of Diplodia pinea on shoots and cones in seed orchards of Pinus spp. in north-western Turkey. Plant Protection Science, 50: 217-220. Doğmuş-Lehtijärvi H.T., Lehtijärvi A., Karaca G., Aday A.G. 2007. Sphaeropsis sapinea Dyko & Sutton associated with shoot blight on Brutian pine in southwestern Turkey. Acta Silvatica & Lignaria Hungarica, Special Edition 2007: 95-99. Kaya A.G.A., Lehtijärvi A., Kaya Ö.D., Doğmuş-Lehtijärvi H.T. 2014. First report of Diplodia pinea on Pseudotsuga menziesii in Turkey. Plant Disease, 98: 689. Nicholls T.H., Ostry M.E. 1990. Sphaeropsis sapinea cankers on stressed red and jack pines in Minnesota and Wisconsin. Plant Disease, 74: 54-56. Palmer M.A., Nicholls T.H. 1985. Shoot blight and col¬lar rot of Pinus resinosa caused by Sphaeropsis sapinea in forest tree nurseries. Plant Disease, 69: 739-740. Peterson G.W. 1977. Infection, epidemiology and control of Diplodia blight of Austrian, Ponderosa and Scots pines. Phytopathology, 67: 511-514. Peterson G.W. 1981. Diplodia Blight of Pines, Forest Insect& Disease Leaflet 161, U.S. Agriculture Forest Service. www.na.fs.fed.us/spfo/pubs/fidls/diplodia/diplodiafidl.htm Rees A.A., Webber J.F. 1988. Pathogenicity of Sphaeropsis sapinea to seed, seedlings and saplings of some Central American pines. Transactions of the British Mycological Society, 91: 273-277. Sinclair W.A., Lyon H.H. 1987. Disease of Trees and Shrubs. Department of Plant Pathology. Cornell Univer¬sity Press, Ithaca, London, 136-137. Stanosz G.R., Blodgett J.T., Smith D.R., Kruger E.L. 2001. Water stress and Sphaeropsis as a latent pathogen of red pine seedlings. New Phytologist, 149: 531-538. Stanosz G.R., Cummings-Carlson J. 1996. Association of mortality of recently planted seedlings and established saplings in red pine plantations with Sphaeropsis collar rot. Plant Disease, 80: 750-753. Sümer S. 2000. Orman Bakanlığı Makamına Kahramanmaraş Yöresi için Hazırlanan Rapor, İstanbul. Swart W.J., Wingfield M.J., Knox-Davies P.S. 1987. Conidial dispersal of Sphaeropsis sapinea in three climat¬ic regions of South Africa. Plant Disease, 71: 1038-1040. White T.J., Bruns T., Lee S., Taylor J.W., 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: M.A. Innis, D.H. Gelfand, J.J. Sninsky White T.J. (Eds.). PCR Protocols: A Guide to Methods and Applications. Academic Press, Inc., New York. pp: 315-322. Wingfield M.J., Know-Davies P.S. 1980. Observation on diseases in pine and eucalyptus plantations in South Africa. Phytophylactica, 12: 57-63.
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Details

Primary Language English
Subjects Structural Biology
Journal Section Articles
Authors

Filiz Ünal

Ercan Koca This is me

Ayşe Aşkın This is me

İlker Kurbetli This is me

Kamil Sarpkaya This is me

Publication Date March 30, 2018
Published in Issue Year 2018 Volume: 31 Issue: 1

Cite

APA Ünal, F., Koca, E., Aşkın, A., Kurbetli, İ., et al. (2018). Identification and virules of Sphaeropsis tip blight (Sphaeropsis sapinea) on Pinus spp. in Istanbul and Bursa parks. Acta Biologica Turcica, 31(1), 17-21.
AMA Ünal F, Koca E, Aşkın A, Kurbetli İ, Sarpkaya K. Identification and virules of Sphaeropsis tip blight (Sphaeropsis sapinea) on Pinus spp. in Istanbul and Bursa parks. Acta Biologica Turcica. March 2018;31(1):17-21.
Chicago Ünal, Filiz, Ercan Koca, Ayşe Aşkın, İlker Kurbetli, and Kamil Sarpkaya. “Identification and Virules of Sphaeropsis Tip Blight (Sphaeropsis Sapinea) on Pinus Spp. In Istanbul and Bursa Parks”. Acta Biologica Turcica 31, no. 1 (March 2018): 17-21.
EndNote Ünal F, Koca E, Aşkın A, Kurbetli İ, Sarpkaya K (March 1, 2018) Identification and virules of Sphaeropsis tip blight (Sphaeropsis sapinea) on Pinus spp. in Istanbul and Bursa parks. Acta Biologica Turcica 31 1 17–21.
IEEE F. Ünal, E. Koca, A. Aşkın, İ. Kurbetli, and K. Sarpkaya, “Identification and virules of Sphaeropsis tip blight (Sphaeropsis sapinea) on Pinus spp. in Istanbul and Bursa parks”, Acta Biologica Turcica, vol. 31, no. 1, pp. 17–21, 2018.
ISNAD Ünal, Filiz et al. “Identification and Virules of Sphaeropsis Tip Blight (Sphaeropsis Sapinea) on Pinus Spp. In Istanbul and Bursa Parks”. Acta Biologica Turcica 31/1 (March 2018), 17-21.
JAMA Ünal F, Koca E, Aşkın A, Kurbetli İ, Sarpkaya K. Identification and virules of Sphaeropsis tip blight (Sphaeropsis sapinea) on Pinus spp. in Istanbul and Bursa parks. Acta Biologica Turcica. 2018;31:17–21.
MLA Ünal, Filiz et al. “Identification and Virules of Sphaeropsis Tip Blight (Sphaeropsis Sapinea) on Pinus Spp. In Istanbul and Bursa Parks”. Acta Biologica Turcica, vol. 31, no. 1, 2018, pp. 17-21.
Vancouver Ünal F, Koca E, Aşkın A, Kurbetli İ, Sarpkaya K. Identification and virules of Sphaeropsis tip blight (Sphaeropsis sapinea) on Pinus spp. in Istanbul and Bursa parks. Acta Biologica Turcica. 2018;31(1):17-21.