Aeromonas hydrophila Suşlarının Antibiyotik Direnç Profilleri

Year 2021, Volume: 17 Issue: 2, 202 - 213, 01.06.2021

Nurdan Filik , Ebru Önem , Ayşegül Kubilay

https://doi.org/10.22392/actaquatr.792224

Cited By: 2

Abstract

Aeromonas hydrophila virülens özellikleri açısından önemli bir patojen olup balıklarda ölümcül bir hastalık olan Motile Aeromonas Septisemisine (MAS) neden olur. Bu çalışmada hasta balıklardan izole edilen 20 A. hydrophila suşunun ve A. hydrophila ATCC 7966 suşunun 28 farklı antibiyotiğe karşı duyarlılığı araştırılmıştır. Araştırmada A. hydrophila suşlarında antibiyotik duyarlılık profilleri Kirby-Bauer disk difüzyon yöntemiyle saptanmış ve heri bir suşa ait Çoklu Antibiyotik Dirençliliği (ÇAD) indeksi hesaplanmıştır. Elde edilen sonuçlara göre suşların 12 antibiyotiğe dirençli, 14 antibiyotiğe duyarlı, 2 antibiyotiğe orta dercede duyarlı olduğu belirlenmiştir. ÇAD indeksi sonuçlarına göre suşların toplam 14 antibiyotiğe karşı çoklu antibiyotik direnci gösterdiği tespit edilmiştir. Suşların ÇAD indeks değerleri 0,21-0,75 aralığında tespit edilmiştir. Son yıllarda yapılan çalışmaların ortaya koyduğu bir bulgu A. hydrophila’nın oksitetrasikline karşı geliştirdiği dirençtir. Bu açıdan araştırma bulgularında en dikkat çeken oksitetrasiklin direnci olmuştur. A. hydrophila suşlarının araştırmada kullanılan antibiyotiklerin büyük bir kısmına ve yapılan bazı çalışmalarla tedavide en etkili antibiyotik olduğu bildirilen oksitetrasikline karşı direnç geliştirdiğinin tespit edilmesi suşların direnç profilinin yüksek olduğunu göstermektedir.
Sonuç olarak, balık patojenlerinin oluşturduğu hastalıkların tedavisinde yanlış antibiyotik kullanımı sonucu patojenin direnç kazandığı, bu durumunda balık hastalıkları ile mücadelede ciddi bir sorun oluşturduğu görülmektedir. 

Keywords

Aeromonas hydrophila, Balık, Antibiyogram, Çoklu Antibiyotik Dirençliliği, CLSI

Supporting Institution

Türkiye Bilimsel ve Teknolojik Araştırma Kurumu (TÜBİTAK)

Project Number

119O671

Thanks

Bu çalışma doktora tezinden özetlenmiştir. Türkiye Bilimsel ve Teknolojik Araştırma Kurumu (TÜBİTAK) 1002 Hızlı Destek Programı kapsamında 119O671 Proje Nosuyla desteklenmiştir. Araştırma da kullanılan A. hydrophila suşları Bursa Uludağ Üniversitesi (BUÜ)’nin, Isparta Uygulamalı Bilimler Üniversitesi (ISUBÜ)’nin, Ondokuz Mayıs Üniversitesi (OMÜ)’nin ve Recep Tayyip Erdoğan Üniversitesi (RTEÜ)’nin değerli Öğretim Elemanları tarafından temin edilmiş olup kendilerinin şahıslarına ve bağlı bulundukları Üniversitelerine teşekkürlerimizi sunarız.

Antibiotic Resistance Profiles of Aeromonas hydrophila Strains

Abstract

Aeromonas hydrophila virulent is a pathogen with important virulent properties and causes Motile Aeromonas Septicemia (MAS), a deadly disease in fish. In this study sensitivity of 20 A. hydrophila strains and A. hydrophila ATCC 7966 strains isolated from sick fish to 28 different antibiotics were investigated. In the study, antibiotic susceptibility profiles of A. hydrophila strains were determined by Kirby-Bauer disk diffusion method and the Multiple Antibiotic Resistance (MAR) index for each strain was calculated. According to the results, it was determined that the strains were resistant to 12 antibiotics, sensitive to 14 antibiotics, and 2 antibiotics moderately. According to the results of the MAR index, the strains were found to have multiple antibiotic resistance against the total of 14 antibiotics. MAR index values of strains were determined in the range of 0.21-0.75. One of the findings of recent studies is the resistance developed by A. hydrophila against oxytetracycline. In this respect, oxytetracycline resistance was the most striking in the research findings. The fact that A. hydrophila strains developed resistance to most of the antibiotics used in the research and oxytetracycline, which was reported to be the most effective antibiotic in treatment, shows that the resistance profile of strains is high.
As a result, it is seen that pathogen has gained resistance as a result of the use of broad-spectrum antibiotics in the treatment of diseases caused by fish pathogens, and this situation creates a serious problem in the fight against fish diseases.

Keywords

Aeromonas hydrophila, Balık, Antibiyogram, Çoklu Antibiyotik Dirençliliği, CLSI

Project Number

119O671

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