Antibiotic and Heavy Metal Resistance of Escherichia coli Strains Isolated from the Seve Dam, and Konak Pond, Kilis, Turkey

Year 2021, Volume: 17 Issue: 2, 290 - 297, 01.06.2021

Hatice Aysun Mercimek Takcı , Sevil Toplar Melis Sumengen Özdenefe

https://doi.org/10.22392/actaquatr.801564

Cited By: 5

Abstract

Pollution of surface waters with coliform bacteria having antibiotic and heavy metal resistance has become an increasing public health risk. For this reason, antibiotic and heavy metal resistance profiles of fecal microorganisms in surface water samples collected from two different points (Seve Dam and Konak Pond, Kilis, Turkey) were analyzed in this study. The various physicochemical parameters such as pH, Temperature, Total Dissolved Solids (TDS), Electrical Conductivity (EC) of samples were also examined. The total coliform load in each sample was ˃1100 (MPN)/100 mL. As fecal indicator microorganisms, 21 Escherichia coli strains were isolated from sampling points. Despite the high coliform contamination, the multiple antibiotic resistance (MAR) index of E. coli was recorded as 0.187. The multiple heavy metal resistance (MHMR) index (0.202) was remarkably higher than the MAR value. Most isolates showed minimal inhibition concentrations (MIC) in the range of 25-1.600 μg/mL based on concentrations of heavy metals. These results indicate that surface waters may be discharge points of industrial and domestic wastewaters

Keywords

Antibiotic, Heavy metal resistance, Coliform pollution, Water quality.

Konak Göleti ve Seve Barajı’ndan, (Kilis, Türkiye) izole edilen Escherichia coli suşlarının antibiyotik ve ağır metal dirençliliği

Abstract

Antibiyotik ve ağır metal dirençliliği gösteren koliform bakterilerle yüzeysel suların kirliliği artan bir halk sağlığı riski oluşturmaktadır. Bu nedenle, çalışmamızda Kilis İlinde iki farklı bölgeden (Seve Barajı ve Konak Göleti) toplanan yüzey suyu örneklerindeki fekal bakterilerin antibiyotik ve ağır metal dirençlilik profilleri araştırılmıştır. Örneklerin pH, sıcaklık, toplam çözünmüş katı madde (TDS) ve elektrik iletkenlik (EC) gibi çeşitli fizikokimyasal değişkenleri incelenmiştir. Her örnekteki total koliform sayısı ˃1100 (EMS)/100 mL’dir. Örnek noktalarında fekal kirliliğin indikatörü olarak 21 Escherichia coli suşu izole edilmiştir. Yüksek koliform kirliliğine karşın, E. coli izolatlarının çoklu antibiyotik direnç (ÇAD) indeksi 0.187 olarak kaydedilmiştir. Çoklu ağır metal direnç (ÇAMD) indeksi (0.202) ise çoklu antibiyotik direncinden yüksek bulunmuştur. Çoğu izolatın 25-1.600 μg/mL aralığında minimum inhibibe edici konsantrasyon gösterdiği belirlenmiştir. Bu sonuçlar yüzey sularının endüstriyel ve evsel atıksuların etkisi altında olabileceğini göstermektedir.

Keywords

Antibiyotik, Ağır metal direnç, Koliform kirliliği, Su kalitesi

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