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Türkiye’de Çift Kabuklu Yumuşakçalarda Betanodavirus Varlığının Araştırılması

Year 2022, Volume Accepted Articles, Issue 1, 15.01.2022
https://doi.org/10.22392/actaquatr.1088276

Abstract

Viral nervöz nekrozis (VNN), özellikle larva ve yavru deniz balıklarında, bazen de yetişkinlerde görülen önemli viral bir hastalıktır. Akdeniz’de artık levreklerde endemik olarak kabul edilen ve sık sık salgınlara neden olan betanodavirusların RGNNV genotipinin yanısıra, son birkaç yılda çipuralarda da salgınlar daha sık bildirilmeye başlanmış ve RGNNV/SJNNV genotipi izole edilmiştir. Bu çalışmanın amacı resmi otoriteden onaylı doğal yataklarda yetiştirilen akivades (Ruditapes decussatus) ve kara midye (Mytilus galloprovincialis) istasyonlarında VNN etkeni betanodavirus varlığının araştırılmasıdır. Çalışmada 2016-2020 yılları arasında beş adet akivades, sekiz adet kara midye istasyonundan toplam 50 örnekleme yapılmıştır. Akivades örneklemesi Ağustos aylarında, kara midye örneklemesi ise Eylül aylarında gerçekleştirilmiştir. Her örneklemede her istasyondan 30 adet örnek alınmıştır ve her biri beş örnekten oluşmak üzere toplam 300 adet havuz oluşturulmuştur. Çift kabuklu yumuşakçaların hepatopankreaslarından hazırlanan örnekler, Real-Time Reverse Transcriptase Polimerase Chain Reaction (RT-qPCR) testi ile betanodavirus yönünden araştırılmıştır. RT-qPCR testleri sonucunda hem akivades örneklerinde hem kara midye örneklerinde betanodavirus nükleik asidi tespit edilmemiştir. Türkiye’de çift kabuklu yumuşakçalarda betanodavirus varlığı ilk defa bu çalışma ile araştırılmıştır. Sonuç olarak bu çalışma ile sadece doğal yataklarda bulunan midye ve akivadeslerde araştırma yapılmıştır, ancak, virüsün daha çok endemik olduğu Güney Ege ve Akdeniz bölgelerinde resmi onaylı akivades veya kara midye istasyonu bulunmadığından bu bölgelerde örnekleme yapılmamıştır. Kabuklularda betanodavirus epidemiyolojisini daha iyi anlamak için bu bölgeleri de içeren daha ileri ve genişletilmiş çalışmalara ihtiyaç vardır.

References

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Investigation of Betanodavirus in Bivalve Mollusks in Turkey

Year 2022, Volume Accepted Articles, Issue 1, 15.01.2022
https://doi.org/10.22392/actaquatr.1088276

Abstract

Viral nervous necrosis (VNN) is an important viral disease that is seen especially in larval and juvenile, occasionally in adult marine fish. VNN is now accepted as endemic in the Mediterranean basin and outbreaks in sea bream caused by RGNNV/SJNNV genotype have been isolated as well as the RGNNV genotype of betanodaviridae which causes frequent outbreaks. The aim of this study was to investigate the presence of the betanodavirus in carpet shell (Ruditapes decussatus) and black mussel (Mytilus galloprovencialis) stations approved by the official authorities in Turkey. A total of 50 samplings were carried out from five carpet shells and eight black mussel stations between 2016-2020. Sampling of carpet shells were conducted in August and black mussels in September by every year. Thirty samples were collected from each station, and a total of three hundred pools consisting of five samples each were produced. Samples prepared from the hepatopancreas of the bivalve mollusks were tested by Real-Time Reverse Transcriptase Polymerase Chain Reaction (RT-qPCR). Betanodavirus nucleic acid was not detected in any of carpet shell and black mussel samples. The presence of betanodavirus in bivalve mollusks in Turkey was investigated for the first time in this study. In conclusion, this study was conducted in only officially approved carpet shell and black mussel stations, however, since there were no officially approved stations in the Southern Aegean and Mediterranean regions, where the virus is more endemic, sampling was not conducted. Further and expanded studies are necessary including these regions to better understand the betanodavirus epidemiology in shellfish.

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  • Baeck, G. W., Gomez, D. K., Oh, K. S., Kim, J. H., Choresca, C. H., & Park, S. C. (2007). Detection of piscine Nodaviruses from apparently healthy wild marine fish in Korea. Bulletin of the European Association of Fish Pathologists, 27, 116–122.
  • Bandín, I., & Souto, S., (2020). Betanodavirus and VER Disease: A 30-year Research Review. Pathogens 9, 106. https://doi:10.3390/pathogens9020106
  • Barker, D. E., MacKinnon, A. M., Boston, L., Burt, M. D., Cone, D. K., Speare, D. J., Griffiths S, Cook M, Ritchie R, & Olivier, G. (2002). First report of piscine nodavirus infecting wild winter flounder Pleuronectes americanus in Passamaquoddy Bay, New Brunswick, Canada. Diseases of Aquatic Organisms, 49(2), 99-105. https://doi:10.3354/dao049099
  • Berzak, R., Scheinin, A., Davidovich, N., Regev, Y., Diga, R., Tchernov, D., & Morick, D. (2019). Prevalence of nervous necrosis virus (NNV) and Streptococcusspecies in wild marine fish and crustaceans from the Levantine Basin, Mediterranean Sea. Diseases of Aquatic Organisms, 133, 7-17. https://doi.org/10.3354/dao03339
  • Bitchava, K., Xylouri, E., Fragkiadaki, E., Athanassopoulou, F., Sabatakou, O., & Papanastassopoulou, M. (2007). First incidence of clinical signs of nodavirus infection in sea bream, Sparus auratus L. The Israeli Journal of Aquaculture, 59, 3–9.
  • Bitchava, K., Chassalevris, T., Lampou, E., Athanassopoulou, F., Economou, V., & Dovas, C. I. (2019). Occurrence and molecular characterization of betanodaviruses in fish and invertebrates of the Greek territorial waters. Journal of Fish Diseases, 42(12), 1773-1783. https://doi.org/10.1111/jfd.13098
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  • Ciulli, S., Galletti, E., Grodzki, M., Alessi, A., Battilani, M., & Prosperi, S. (2007). Isolation and genetic characterization of Betanodavirus from wild marine fish from the Adriatic Sea. Veterinary Research Communications, 31(Suppl 1), 221–224. https://doi.org/10.1007/s11259-007-0010-y
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Details

Primary Language Turkish
Subjects Biology
Journal Section Research Articles
Authors

Murat KAPLAN> (Primary Author)
İzmir/Bornova Veteriner Kontrol Enstitüsü
0000-0002-2634-6478
Türkiye


Kemal PEKMEZ>
İzmir/Bornova Veteriner Kontrol Enstitüsü
0000-0001-7077-6582
Türkiye


Abdurrahman Anıl ÇAĞIRGAN>
İzmir/Bornova Veteriner Kontrol Enstitüsü
0000-0001-7766-3150
Türkiye


Buket ÖZKAN>
İzmir/Bornova Veteriner Kontrol Enstitüsü
0000-0003-3464-5651
Türkiye


Fatih ARSLAN>
İzmir/Bornova Veteriner Kontrol Enstitüsü
0000-0002-6706-3650
Türkiye


Bülent KAFA>
İzmir/Bornova Veteriner Kontrol Enstitüsü
0000-0002-1402-894X
Türkiye


Gülnur KALAYCI>
İzmir/Bornova Veteriner Kontrol Enstitüsü
0000-0002-2024-303X
Türkiye

Supporting Institution İzmir/Bornova Veteriner Kontrol Enstitüsü
Thanks Yazarlar, desteklerinden ve teşviklerinden dolayı İzmir/Bornova Veteriner Kontrol Enstitüsü Müdürlüğü’ne teşekkür etmektedir.
Publication Date January 15, 2022
Published in Issue Year 2022, Volume Accepted Articles, Issue 1

Cite

APA Kaplan, M. , Pekmez, K. , Çağırgan, A. A. , Özkan, B. , Arslan, F. , Kafa, B. & Kalaycı, G. (2022). Türkiye’de Çift Kabuklu Yumuşakçalarda Betanodavirus Varlığının Araştırılması . Acta Aquatica Turcica , Accepted Articles (1) , . DOI: 10.22392/actaquatr.1088276