İnaktive Edilmiş Streptococcus iniae, Edwardsiella tarda ve Poly I:C'nin Japon Pisi Balığındaki CXCL-10 ve CXCL-9 Geninin mRNA Ekspresyon Seviyeleri Üzerindeki Etkileri

Year 2024, Volume: 20 Issue: 2, 128 - 139, 01.06.2024

Ergi Bahrioğlu Hidehiro Kondo Ikuo Hırono

https://doi.org/10.22392/actaquatr.1312305

Abstract

Bu çalışma, Japon pisi balığının (Paralichthys olivaceus) dalak cDNA'sında JfCXCL9_L ve JfCXCL10_L genlerinin başarılı bir şekilde klonlandığını ve dizilendiğini bildirmektedir. Bu iki genin dokulardaki dağılımları da herhangi bir stimulant uygulanmadan önce (0h) belirlenmiştir. Ayrıca, qRT-PCR analizi, JfCXCL10_L ekspresyonlarının IL1-β ile benzerlik gösterdiğini ortaya koymuştur. Poly I:C ve formalinle öldürülmüş Edwardsiella tarda (FKCET) uygulamasından sonra artan ekspresyon seviyesi gösterirken, formalinle öldürülmüş Streptococcus iniae (FKCSI) uygulamasından sonra azalan ekpresyon seviyesi tespit edilmiştir. Bu durum, JfCXCL10_L'nin viral ve gram-negatif bakteriyel uyarılara karşı bağışıklık tepkisinde rolü olduğunu düşündürmektedir. JfCXCL9_L mRNA düzeyleri, FKCET ve FKCSI uygulamalarından sonra anlamlı derecede düşmüştür. Özellikle belirli zaman noktalarında, JfCXCL9_L düzeyleri Poly I:C uygulamasına kıyasla daha düşük bulunmuştur. Bu bulgular, JfCXCL9_L ve JfCXCL10_L'nin Japon pisi balığında bağışıklık tepkisi mekanizmaları ve potansiyel işlevleri konusunda bilgi sunmaktadır.

Keywords

CXC Motifi, Kemokin, Poly I:C, Paralichthys olivaceus, Gen Ekspresyonu

Effects of inactivated Streptococcus iniae, Edwardsiella tarda, and Poly I:C on mRNA Expression Levels of CXCL-10 and CXCL-9 Genes in Japanese Flounder

Abstract

The present study accomplished the successful cloning and sequencing of the JfCXCL9_L and JfCXCL10_L genes found in the spleen cDNA of the Japanese flounder (Paralichthys olivaceus). The tissue distribution of these two genes was determined before any stimuli administration at the zero-hour mark. The qRT-PCR analysis demonstrated that the expression of JfCXCL10_L closely mirrored that of IL1-β, displaying an upregulation following the application of Poly I:C (Viral mimic) and formalin-killed Edwardsiella tarda (Gram-Negative mimic), while showing a downregulation after the application of formalin-killed Streptococcus iniae (Gram-Positive mimic) treatment. These findings strongly suggest a role for JfCXCL10_L in the immune response to viral and gram-negative bacterial stimuli. Regarding JfCXCL9_L, mRNA levels were found to be significantly downregulated after FKCET and FKCSI treatments, though to varying extents. Interestingly, at specific time points, JfCXCL9_L levels were even lower compared to Poly I:C treatment. These intriguing findings shed valuable light on the roles of both JfCXCL9_L and JfCXCL10_L in potential functions of immune response mechanisms of the Japanese flounder'.

Keywords

CXC Motif, Chemokine, Poly I:C, Paralichthys olivaceus, Gene Expression

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