The Effect of Uterine Lavage and Oxytocin Administration Before and After Breeding on Fertility in Mares in The First Postpartum Estrus

Year 2018, Volume: 44 Issue: 3, 112 - 116, 02.09.2018

Mert Kundak Mehmet Ragıp Kılıçarslan

Abstract

DOI: 10.26650/actavet.2019.398037

The objective of this study was to
investigate the effects of the combination of uterine lavage and oxytocin
administration before and after breeding during post-partum first estrus on the
pregnancy rates of mares. Thirty mares whose fetal membranes were released
within the first 3 h after parturition were divided into three groups - the
control (n=10), pre-breeding (n=10) and post-breeding treatment group (n=10).
The uterine lavage was performed 4 h before breeding in the pre-breeding group
and 4 h after breeding in the post-breeding group. The oxytocin administration
was performed twice in both treatment groups intravenously - immediately after
and 12 h after the uterine lavage. A sterile NaCl solution (0.9%) was
administrated intravenously in the control group.  In the control group there was a longer
interval between parturition and first ovulation (14.6 days) compared to
treatment groups (p<0.05). The pregnancy rates in the control; pre-breeding
and post-breeding treatment groups were calculated as 40%, 40%, and 60%,
respectively. Although early embryonic loss was not observed in both the pre-
and post-breeding treatment groups, this ratio was 25%  for the control group. As a conclusion, the
administration of a uterine lavage (1 liter of sterile 0.9% NaCl solution
+4.000.000 IU crystallized penicillin +4g streptomycin sulfate) and 20 IU
oxytocin 4 h before or after breeding mares at their first postpartum ovulation
shortens the day interval between parturition and ovulation. It can be assumed
that breeding during foal heat can be effective in reducing uterine involution,
inflammatory reactions related to breeding and embryonic death.

Keywords

foal heat, lavage, ovulation period, oxytocin, pregnancy rates

References

• Azawi, O.J., 2008. Effect on Fertility of Human Chorionic Gonadotrophin and Uterine Lavage with Oxytocin Performed After Mating in Arabian Barren Mares. Animal Reproduction Science 106, 345-351.
• Ball, B.A., 1993. Embryonic Death in Mares. In: McKinnon, A.O., Voss, J.L. (Eds.), Equine Reproduction. Lea & Febiger, Philadelphia, pp. 517-527.
• Blanchard, T.L., Varner, D.D., 1993. Uterine Involution and Postpartum Breeding. In: McKinnon, A.O., Voss, J.L. (Eds), Equine Reproduction. Lea&Febiger, Philadelphia,pp. 622-625.
• Bliss, A.J., Campbell, M.L.H., 2010. Evaluation of the Efficacy of Three Diffrent Doses of Oxytocin on Uterine Fluid Clearance in Mares. Animal Reproduction Science 121, 118-119.
• Bozkurt, Z., 2007. Kısraklarda Aşım Öncesi ve Sonrası Jinekolojik Muayeneler ile Tedavi Girişimlerinin Fertiliteye Etkisi. Doktora Tezi, Ankara Üniversitesi Sağlık Bilimleri Enstitüsü, Ankara.
• Brinsko, S.P., Varner, D.D., Blanchard, T.L. ,1991. The Effect of Uterine Lavage Performed Four Hours Post Insemination on Pregnancy Rate in Mares. Theriogenology 35, 1111-1119.
• Brinsko, S.P., 2001. How to Perform Uterine Lavage: İndications and Practical Techniques. AAEP Proceedings 47, 407-411.
• Cadario, M.E., Merritt, A.M., Archbald, L.F., Thatcher, W.W., Leblanc, M.M., 1999. Changes in İntrauterine Pressure After Oxytocin Administration in Reproductively Normal Mares and in those with a Delay in Uterine Clearance. Theriogenology 51, 1017-1025.
• Dadarwala, D., Tandonb, S.N., Purohitc, G.N. Pareekc, P.K., 2004. Ultrasonographic evaluation of Uterine Involution and Postpartum Follicular Dynamics in French Jennies. Theriogenology 62, 257-264.
• Gündüz, M.C., Kaşıkçı, G., Horoz, H., 2008. The Effect Of Oxytocin And Pgf2alpha On The Uterine Involution And Pregnancy Rates In Postpartum Arabian Mares. Animal Reproduction Science 104, 257-263.
• Katila, T., Reilas, T., 2001. The Post Partum Mare. Pferdeheilkunde 17, 623-626.
• Keskintepe, L., Alpar, R., Küplülü, Ş., 1988. Çifteler Anadolu Tarım İşletmesindeki Safkan Arap Kısrakların Bazı Reprodüktif Özellikleri Üzerinde İncelemeler. Ankara Üniversitesi Veteriner Fakültesi Dergisi 35, 488-496.
• Kılıçarslan, M.R., 2002. Kısraklarda bir GnRH analoğu olan Deslorelin acetate ile ovulasyonun uyarılması. İstanbul Üniversitesi Veteriner Fakültesi Dergisi 28, 403-409.
• Kılıçarslan, M.R., 2013. Kısraklarda üremenin denetlenmesi. In: V. Veteriner Doğum ve Jinekoloji Kongresi, Antalya, 22-23.
• Kılıçarslan, M.R., Horoz, H., Şenünver, A., Konuk, S.C., Tek, Ç., Carıoğlu, B., 1996. Effect of GnRH and hCG on ovulation and pregnancy in mares. Veterinary Record 139, 119-120.
• Kılıçarslan, M.R., Uçar, M., 2015. Genital Organların Muayenesi. In: Kaymaz, M., Fındık, M., Rişvanlı, A., Köker, A. (Eds), Kısraklarda Doğum ve Jinekoloji. Medipres, Malatya, pp. 45-81.
• Le Blanc, M.M., 2009. Delay in Uterine Clearance or Chronic Endometritis-Which Does the Mare Have and How Sould it be Treated?, http://www.ivis.org/proceedings/weva/2009/478.pdf?LA=1 (Erişim 19.02.2018)
• Le Blanc, M.M., 2003. Persistent Mating Induced Endometritis in the Mare: Pathogenesis and Treatment, http://www.ivis.org/advances/Reproduction_Ball/leblanc/chapter.asp?LA=1 (Erişim 19.02.2018)
• Lowis, T.C., Hyland, J.H., 1991. Analysis of Postpartum Fertility in Mares on a Thoroughbred Stud in Outhern Victoria. Australian Veterinary Journal 68, 304-306.
• Loy, R.G., 1980. Characteristics of Postpartum Reproduction in Mares. Veterinary Clinics of North America: Large Animal Practice 2, 345-359.
• Malschitzky, E., Schilela, A., Mattos, A.L.G., Garbade, P., Gregory, R.M., Mattos, R.C., 2002. Effect of Intra-Uterine Fluid accumulation During and After Foal-Heat and of Different Management Techniques on the Postpartum Fertility of Throughbred Mares. Theriogenology 58, 495-498.
• McKinnon, A.O., Squires, E.L., Harrison, L.A., Blach, E.L., Shideler, R.K., 1988. Ultrasonographic Studies on the Reproductive Tract of Mares After Parturrition: Effect of Involution and Uterine Fluid on Pregnancy Rates in Mares with Normal and Delayed First Postpartum Ovulatory Cycles. Journal of the American Veterinary Medical Association 192, 350-353.
• Nagy, P., Guillaume, D., Daels, P., 2000. Seasonality in mares. Animal Reproduction Science 2, 245-262.
• Pycock, J., 2001. İnfertility in the Mare. In: Noakes, D.E., Parkinson, T.J., England, G.C.W. (Eds), Veterinary Reproduction and Obstetrics. Saunders, Philadelpia, pp. 577-670.
• Pycock, J.F., Newcombe, J.R., 1996. Assesment of the Effect of Three Treatments to Remove Intrauterine Fluid on Pregnancy Rate in the Mare. Veterinary Record 138, 320-323.
• Reilas, T., Katila, T., Makela, O., Huhtinen, M., Koskinen, E., 1997. Intrauterine Fluid Accumulation in Oestrus Mares. Acta Veterinaria Scandinavica 38, 69-78.
• Sertich, P.L., Watson, E.D., 1992. Plasma concetrations 15-keto-13,14-dihidro-PGF2alfa in Mares During Uterine Involution, Journal of the American Veterinary Medical Association 201, 434-437.
• Watson, E.D., 2000. Postbreeding endometritis in the Mare, Animal Reproduction Science 2, 221-232.