Determination of Effective Mutation Dose on Walnut (Juglans regia L. cv. Chandler) Budwoods

Year 2021, Volume: 18 Issue: 1, 111 - 117, 30.06.2021

Sinem Sanlı Zeynel Dalkılıç

https://doi.org/10.25308/aduziraat.859402

Cited By: 1

Abstract

Walnut (Juglans regia L., 2n=2x=32, Juglandaceae) is a deciduous temperate fruit species with an increasing economic importance and health benefits. The effective mutation dose (EMD) was not determined for ‘Chandler’ budwoods. The objective of this study was to determine the radiosensitivity of walnut budwoods to cobalt-60 gamma ray. One-year-old budwoods of ‘Chandler’ walnut cultivar carrying 4-5 buds in 20 cm in length were irradiated with cobalt-60. The gamma irradiation and chip budding were performed three different times. The budwoods were irradiated (1) with 0, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100 Gy gamma doses on Apr 2017, (2) with 0, 20, 25, 30, 35, 40 Gy gamma doses on Sep 2017, and (3) with only 42 Gy gamma dose on Apr 2018. Then, gamma irradiated budwoods were chip budded on seedling rootstocks. Mutation 1 Vegetation 1 (M1V1) plants were obtained. The humidity content of the budwoods was determined after gamma irradiation. The bud take ratio, shoot length, and chlorophyll density of M1V1 plants were measured. From the shoot length of the plants, EMD was calculated as 42.1 Gy after the first irradiation on Apr, 2017, and that was calculated as 20.9 Gy in the second irradiation on Sep, 2017. The main plant population was obtained by previously calculated 42.1 Gy dose in the third irradiation on Apr, 2018. The survival rates of budded plants in three irradiation experiments were 21.6%, 54.8%, and 32.0%, respectively. According to the results, the most suitable gamma ray dose is 42.1 Gy.

Keywords

dormant budding, budwood, radiosensitivity, fruit breeding, physical mutagen, gamma irradiation

Supporting Institution

Aydın Adnan Menderes Üniversitesi

Project Number

ADU BAP ZRF-17023

Thanks

We thank Dr. Burak Kunter and Prof. Dr. Kourosh Vahdati for their critical reading of the manuscript. ADU-AgBioCenter was acknowledged for allowing us to conduct a part of this work. This M.S. thesis work was funded by the Scientific Research Project No. ADU BAP ZRF-17023

Ceviz (Juglans regia L. cv. Chandler) Aşı Gözleri Üzerine Etkili Mutasyon Dozunun Belirlenmesi

Abstract

Ceviz (Juglans regia L., 2n=2x=32, Juglandaceae), ekonomik önemi ve sağlık açısından faydaları artan, yaprak döken ılıman iklim meyve türüdür. ‘Chandler’ aşı gözlerinin etkili mutasyon dozu (EMD) henüz belirlenmemiştir. Bu çalışmanın amacı, ceviz aşı gözlerinin kobalt-60 ışınlamasına hassasiyetini belirlemektir. Üzerinde 4-5 aşı gözü bulunan 20 cm uzunluğundaki ‘Chandler’ ceviz çeşidi aşı kalemleri kobalt-60 ışınlamasına maruz bırakılmıştır. Üç farklı dönemde gama ışınlaması ve yongalı göz aşısı yapılmıştır. Aşı kalemleri (1) Nisan 2017’de 0, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100 Gy gama dozlarına, (2) Eylül 2017’de 0, 20, 25, 30, 35, 40 Gy gama dozlarına ve (3) Nisan 2018’de 42 Gy gama dozuna tabi tutulmuşlardır. Gama uygulaması yapılan aşı kalemleri çöğür anaçları üzerine yongalı göz aşısı ile aşılanmıştır. Mutasyon 1 Vejetasyon 1 (M1V1) bitkileri elde edilmiştir. Aşı kalemlerinin nem içeriği gama ışınlamasından sonra belirlenmiştir. M1V1 bitkilerinin aşı tutma oranı, sürgün boyu ve klorofil yoğunluğu ölçülmüştür. Aşılamadan elde edilen bitkilerin sürgün uzunluklarından, birinci ışınlamadaki EMD=42.1 Gy olarak ve ikinci ışınlamadaki EMD=20.9 Gy olarak belirlenmiştir. Üçüncü ışınlama döneminde sadece daha önce belirlenen 42.1 Gy dozu uygulanılarak ana populasyon bitkileri oluşturulmuştur. Üç ışınlama denemesinden elde edilen aşılı bitki yaşama oranları sırasıyla %21.6, %54.8 ve %32.0. Bu sonuçlara göre, ‘Chandler’ aşı kalemlerine yapılacak en uygun gama dozu 42.1 Gy’dir.

Keywords

durgun yongalı göz aşısı, aşı gözü, radyasyon hassasiyeti, meyve ıslahı, fiziksel mutajen, gama ışınlaması

Project Number

ADU BAP ZRF-17023

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