The Effect of Delivery Mode on Thiol-Disulfide Balance in the Umbilical Cord Blood

Year 2021, Volume: 5 Issue: 1, 22 - 27, 20.04.2021

Selda Songur Dağlı Recai Dağlı

https://doi.org/10.46332/aemj.841513

Abstract

Purpose: Today, the Oxidative stress level is frequently determined through the methods examining the thiol-disulfide balance. We aimed to compare oxidative stress in elective caesarean sections (ECS) and vaginal deliveries (VD) by examining the thiol-disulfide balance.

Materials and methods: Forty- eight VDs and 57 ECSs were included in the study. For standardization, pregnant women who underwent spinal anesthesia for ECS were examined in the study. Five milliliter of blood samples were taken from the umbilical artery immediately after placenta delivery. Serum native thiol, total thiol, disulfide was analyzed in blood samples. Disulfide/native thiol (%), disulfide/total thiol (%), and native thiol/total thiol (%) ratios were calculated.

Results: Native thiol, total thiol, disulfide, and disulfide/total thiol ratios were significantly lower in the ECS group than in the VD group (446.27±48.01 vs 484.26±47.50, p<0.001, 493.28±69.14 vs 541.43±64.21, p<0.001, 23.75±9.27 vs. 28.58±11.11, p=0.017, and 4.68±1.48 vs 5.80±2.04, p= 0.002, respectively). There were no differences between the groups in terms of disulfide/native thiol, and native thiol/total thiol ratios (p>0.05). There were no differences between the groups in terms of mean age, gestational week, and birth weight (p>0.05). First and 5th Apgar scores of the newborn of the ECS group were higher than the VD group (p<0.01).

Conclusion: The thiol-disulfide balance is affected by delivery mode. The oxidative stress level in vaginal deliveries is higher than in elective caesarean sections.

Keywords

caesarean section, disulfides, oxidative stress, thiols, vaginal delivery

Supporting Institution

Yok

Project Number

Yok

Thanks

We would like to thank the team of biochemistry laboratory for the assistance in this research.

Doğum Şeklinin Umbilikal Kord Kanındaki Tiyol-Disulfid Dengesine Etkisi

Abstract

Amaç: Günümüzde, oksidatif stress seviyesi sıklıkla thiol-disufid dengesini inceleyen metodlar yoluyla saptanır. Elektif sezaryen (ES) ve vajinal doğumlardaki (VD) oksidatif stresi tihol-disulfid dengesini inceleyerek karşılaştırmayı amaçladık.

Araçlar ve Yöntem: Çalışmaya 48 VD ve 57 ES dahil edildi. Çalışmada, standardizasyon amacıyla, spinal anestezi ile yapılan ES’lar incelendi. Plasenta ayrıldıktan hemen sonra umblikal arterden beş mililitre kan örneği alındı. Serum nativ thiol, total thiol, disulfid analiz edildi. Disulfid/nativ thiol (%), disulfid/total thiol (%), and nativ thiol/total thiol (%) oranları hesaplandı.

Bulgular: ES grubundaki nativ thiol, total thiol, disulfid, and disulfid/total thiol oranları VD grubundan belirgin olarak düşüktü (446.27±48.01 , 484.26±47.50, p<0.001, 493.28±69.14 , 541.43±64.21, p<0.001, 23.75±9.27 , 28.58±11.11, p=0.017 ve 4.68±1.48 , 5.80±2.04, p= 0.002, sırasıyla). Gruplar arasında disulfid/nativ thiol ve nativ thiol/total thiol oranları bakımından farklılık yoktu (p>0.05).
Gruplar arasında yaş ortalaması, doğum haftası ve doğum ağırlığı açısından fark yoktu (p>0.05). ES grubundaki yenidoğanların ilk ve 5. dakika Apgar skorları VD grubundan daha yüksekti (p<0.01).

Sonuç: Thiol-disulfit dengesi doğum yönteminden etkilenmektedir. Vajinal doğumdaki oxidativ stres seviyesi elektif sezaryendekinden daha yüksektir.

Keywords

sezeryan, disülfit, oksidatif stres, tiyol, vajinal doğum

Project Number

Yok

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