In Vitro rapid propagation of an aquatic plant Pogostemon erectus (Dalzell) Kuntze

Year 2019, Volume: 3 Issue: 1, 1 - 6, 01.05.2019

Muhammet Doğan

https://doi.org/10.30616/ajb.441197

Cited By: 8

Abstract

The aim of this study is to
investigate the multiple and rapid production of Pogostemon erectus (Dalzell) Kuntze by tissue culture techniques. The shoot tip and nodal explants of P. erectus were isolated and then
cultured in Murashige and Skoog (1962) (MS) nutrient media containing
thidiazuron (TDZ) and 2,4-Dichlorophenoxyacetic acid (2,4-D) in different
combinations. The first shoot formation on the explants was observed on day 15.
The number of shoots per explant ranged from 2.85 to 29.39 in the shoot tip
explants and from 2.72 to 32.24 in the nodal explants. The maximum number of
shoots per explant was obtained in the MS medium containing 0.20 mg/L TDZ +
0.10 mg/L 2,4-D for shoot tip explant (29.39) and in the MS medium containing
0.10 mg/L TDZ + 0.10 mg/L 2,4-D for nodal explant (32.24). The longest shoots in the shoot
tip (1.76 cm) and nodal (1.64 cm) explants were determined in MS medium
supplemented with 0.10 mg/L TDZ + 0.10 mg/L 2,4-D. Regenerated shoots were
rooted in MS medium supplemented with 0.25 mg/L indol-3yl acetic acid (IAA), and then acclimatized to
aquarium conditions.

Keywords

In vitro, Shoot regeneration, TDZ, Tissue culture, Pogostemon erectus, Shoot regeneration, TDZ, Tissue culture

Sucul bir bitki olan Pogostemon erectus (Dalzell) Kuntze’un In Vitro hızlı çoğaltımı

Abstract

Bu çalışmanın amacı, Pogostemon erectus (Dalzell) Kuntze'un
doku kültürü teknikleri ile çoklu ve hızlı üretimini araştırmaktır. P. erectus'un sürgün ucu ve nodal
eksplantları izole edilmiş ve daha sonra farklı kombinasyonlarda thidiazuron
(TDZ) ve 2,4-Diklorofenoksiasetik asit (2,4-D) içeren Murashige ve Skoog (1962)
(MS) besin ortamında kültüre alınmıştır. Eksplantlardaki ilk sürgün oluşumu 15.
günde gözlenmiştir. Eksplant başına sürgün sayısı, sürgün ucu eksplantlarında
2.85 ila 29.39 ve nodal eksplantlarında 2.72 ila 32.24 arasında sıralanmıştır.
Eksplant başına maksimum sürgün sayısı sürgün ucu eksplantı için (29.39) 0.20
mg/L TDZ + 0.10 mg/L 2,4-D içeren MS ortamında ve nodal eksplant için 0.10 mg/L
TDZ + 0.10 mg/L 2,4-D içeren MS ortamında (32.24) elde edilmiştir. En uzun
sürgünler, sürgün ucu (1.76 cm) ve nodal (1.64 cm) eksplantlarında 0.10 mg/L
TDZ + 0.10 mg/L 2,4-D ile desteklenmiş MS ortamında belirlenmiştir. Rejenere
sürgünler, 0.25 mg/L Indol-3-asetik asit (IAA) ile takviye edilmiş MS ortamında
köklendirilmiş ve ardından akvaryum koşullarına alıştırılmıştır.

Keywords

In vitro, Sürgün rejenerasyonu, TDZ, Pogostemon erectus, TDZ, Doku kültürü

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