Sucul bir bitki olan Pogostemon erectus (Dalzell) Kuntze’un In Vitro hızlı çoğaltımı

Year 2019, Volume: 3 Issue: 1, 1 - 6, 01.05.2019

Muhammet Doğan

https://doi.org/10.30616/ajb.441197

Cited By: 9

Abstract

Bu çalışmanın amacı, Pogostemon erectus (Dalzell) Kuntze'un doku kültürü teknikleri ile çoklu ve hızlı üretimini araştırmaktır. P. erectus'un sürgün ucu ve nodal eksplantları izole edilmiş ve daha sonra farklı kombinasyonlarda thidiazuron (TDZ) ve 2,4-Diklorofenoksiasetik asit (2,4-D) içeren Murashige ve Skoog (1962) (MS) besin ortamında kültüre alınmıştır. Eksplantlardaki ilk sürgün oluşumu 15. günde gözlenmiştir. Eksplant başına sürgün sayısı, sürgün ucu eksplantlarında 2.85 ila 29.39 ve nodal eksplantlarında 2.72 ila 32.24 arasında sıralanmıştır. Eksplant başına maksimum sürgün sayısı sürgün ucu eksplantı için (29.39) 0.20 mg/L TDZ + 0.10 mg/L 2,4-D içeren MS ortamında ve nodal eksplant için 0.10 mg/L TDZ + 0.10 mg/L 2,4-D içeren MS ortamında (32.24) elde edilmiştir. En uzun sürgünler, sürgün ucu (1.76 cm) ve nodal (1.64 cm) eksplantlarında 0.10 mg/L TDZ + 0.10 mg/L 2,4-D ile desteklenmiş MS ortamında belirlenmiştir. Rejenere sürgünler, 0.25 mg/L Indol-3-asetik asit (IAA) ile takviye edilmiş MS ortamında köklendirilmiş ve ardından akvaryum koşullarına alıştırılmıştır.

Keywords

In vitro, Sürgün rejenerasyonu, TDZ, Pogostemon erectus, TDZ, Doku kültürü

In Vitro rapid propagation of an aquatic plant Pogostemon erectus (Dalzell) Kuntze

Abstract

The aim of this study is to investigate the multiple and rapid production of Pogostemon erectus (Dalzell) Kuntze by tissue culture techniques. The shoot tip and nodal explants of P. erectus were isolated and then cultured in Murashige and Skoog (1962) (MS) nutrient media containing thidiazuron (TDZ) and 2,4-Dichlorophenoxyacetic acid (2,4-D) in different combinations. The first shoot formation on the explants was observed on day 15. The number of shoots per explant ranged from 2.85 to 29.39 in the shoot tip explants and from 2.72 to 32.24 in the nodal explants. The maximum number of shoots per explant was obtained in the MS medium containing 0.20 mg/L TDZ + 0.10 mg/L 2,4-D for shoot tip explant (29.39) and in the MS medium containing 0.10 mg/L TDZ + 0.10 mg/L 2,4-D for nodal explant (32.24). The longest shoots in the shoot tip (1.76 cm) and nodal (1.64 cm) explants were determined in MS medium supplemented with 0.10 mg/L TDZ + 0.10 mg/L 2,4-D. Regenerated shoots were rooted in MS medium supplemented with 0.25 mg/L indol-3yl acetic acid (IAA), and then acclimatized to aquarium conditions.

Keywords

In vitro, Shoot regeneration, TDZ, Tissue culture, Pogostemon erectus, Shoot regeneration, TDZ, Tissue culture

References

• Ahmed MR, Anis M (2012). Role of TDZ in the Quick Regeneration of Multiple Shoots From Nodal Explant of Vitex trifolia L.-an important medicinal plant. Appl Biochem Biotechnol 168: 957-966.
• Baskaran P, Kumari A, Van Staden J (2017). In vitro Propagation Via Organogenesis and Synthetic Seeds of Urginea altissima (L.f.) Baker: a Threatened Medicinal Plant. 3 Biotech 8:18.
• Bourrain L (2018). In Vitro Propagation of Actinidia melanandra Franch. and Actinidia rubricaulis Dunn. from Shoot Tip Explants. N Z J Crop Hortic Sci 46(2): 162-173.
• Dobranszki J, Asboth G, Homoki D, Biro-Molnar P, da Silva JAT, Remenyik J (2017). Ultrasonication of In Vitro Potato Single Node Explants: Activation and Recovery of Antioxidant Defence System and Growth Responses. Plant Physiol Biochem 121: 153-160.
• Dogan M (2017a). Multiple Shoot Regeneration from Shoot Tip and Nodal Explants of Rotala rotundifolia (Buch-Ham. ex Roxb) Koehne. Ant J Bot 1(1): 4-8.
• Doğan M (2017b). In Vitro Koşullarda Çoğaltılan Bazı Su Bitkilerinin Fitoremediasyon Potansiyellerinin Araştırılması. Karamanoğlu Mehmetbey Üniversitesi, Fen Bilimleri Enstitüsü, Biyoloji Anabilim Dalı, Karaman.
• Emsen B, Dogan M (2018). Evaluation of Antioxidant Activity of In Vitro Propagated Medicinal Ceratophyllum demersum L. Extracts. Acta Sci Pol Hortoru 17(1): 23-33.
• George EF (2008). Plant Tissue Culture Procedure - Background, Plant Propagation by Tissue Culture 3rd Edition, Editörler: George, E.F., Hall, M. ve De Klerk, G.J. 1, Springer, Netherlands.
• Gnanaraj WE, Marimuthu J, Subramanian KM, Nallyan S (2011). Micropropagation of Alternanthera sessilis (L.) Using Shoot Tip and Nodal Segments. Iran J Biotechnol 9: 206-212.
• Huetteman CA, Preece JE (1993). Thidiazuron: a Potent Cytokinin for Woody Plant Tissue. Plant Cell Tissue Organ Cult 33: 105-119.
• Hussain SA, Ahmad N, Anis M (2018). Synergetic effect of TDZ and BA on Minimizing the Post-Exposure Effects on Axillary Shoot Proliferation and Assessment of Genetic Fidelity in Rauvolfia tetraphylla (L.). Rend Lincei Sci Fis Nat 29: 109-115.
• Hussain A, Qarshi IA, Nazir H, Ullah I (2012). Plant Tissue Culture: Current Status and Opportunities, Recent Advances in Plant in vitro Culture, Editörler: Leva, A. ve Rinaldi, L.M.R., 1, InTech, Croatia.
• Irshad M, Rizwan HM, Debnath B, Anwar M, Li M, Liu S, He BZ, Qiu DL (2018). Ascorbic Acid Controls Lethal Browning and Pluronic F-68 Promotes High-frequency Multiple Shoot Regeneration from Cotyldonary Node Explant of Okra (Abelmoschus esculentus L.). HortScience 53(2): 183-190.
• Kadota M, Niimi Y (2003). Efects of Cytokinin Types and Their Concentrations on Shoot Proliferation and Hyperhydricity in In Vitro Pear Cultivar Shoots. Plant Cell Tissue Organ Cult 72: 261-265.
• Krishnan JJ, Gangaprasad A, Satheeshkumar K (2018). In Vitro Mass Multiplication and Estimation of Camptothecin (CPT) in Ophiorrhiza mungos L. var. angustifolia (Thw.) Hook. f.. Ind Crops Prod 119: 64-72.
• Kumar V, Moyo M, Van Staden J (2016). Enhancing Plant Regeneration of Lachenalia viridiflora, a Critically Endangered Ornamental Geophyte with High Floricultural Potential. Sci Hortic 211: 263-268.
• Mahindrakar A, Kumar R, Aswath C, Munikrishnappa PM (2018). Callusing and Regeneration Response of In Vitro Derived Leaf Explants of Gerbera (Gerbera jamesonii). Indian J Agricul Sci 88(6): 860-864.
• Mahoney J, Apicella PV, Brand MH (2018). Adventitious Shoot Regeneration from In Vitro Leaves of Aronia mitschurinii and Cotyledons of Closely Related Pyrinae taxa. Sci Hortic 237: 135-141.
• Murashige T, Skoog F (1962). A Revised Medium for Rapid Growth and Bioassays With Tobacco Tissue Cultures. Plant Physiol 15: 473-497.
• Nazi S, Siddiquiland MF, Raza S (2018). Effect of Different Growth Regulators on In Vitro Propagation of Brassica napus L.. Pak J Bot 50(5): 1871-1876.
• Neumann KH, Kumar A, Imani J (2009). Plant Cell and Tissue Culture - A Tool in Biotechnology, Principles and Practice, Springer-Verlag Berlin Heidelberg, 1, Germany.
• Sasidharan P, Jayachitra A, (2017). Direct Shoot Bud Regeneration from Shoot Tip Explants of Enicostema axillare: An Important Medicinal Plant. Agrofor Syst 91(3): 471-477.
• Sharma S, Gambhir G, Srivastava DK (2017). In Vitro Differentiation and Plant Regeneration from Root and Other Explants of Juvenile Origin in Pea (Pisum sativum L.). Legume Res 40(6): 1020-1027.
• Shekhawat MS, Manokari M (2018). In Vitro Multiplication, Micromorphological Studies and Ex Vitro Rooting of Hybanthus enneaspermus (L.) F. Muell. - A Rare Medicinal Plant. Acta Bot Croat 77(1): 80-87.
• Snedecor GW, Cochran WG (1997). Statistical Methods. The Iowa State University Press, Iowa. USA.
• Thul ST, Kukreja AK, (2010). An Efficient Protocol for High-frequency Direct Multiple Shoot Regeneration from Internodes of Peppermint (Mentha x piperita). Nat Prod Commun 5(12): 1945-1946.
• Zhang YY, Chen YL, Zhang XH, da Silva JAT, Ma GH (2017). Adventitious Shoot Induction from Internode and Root Explants in a Semiparasitic Herb Monochasma savatieri Franch ex Maxim. J Plant Growth Regul 36(3): 799-804.