Investigation of the toxicity of ethanol extracts obtained from six different Satureja L. species on Colorado Potato Beetle, Leptinotarsa decemlineata (Say, 1824), (Coleoptera: Chrysomelidae)

Year 2019, Volume: 3 Issue: 2, 69 - 79, 01.11.2019

Ayşe Usanmaz Bozhüyük Şaban Kordali

https://doi.org/10.30616/ajb.623827

Cited By: 5

Abstract

In the
present study, ethanol extracts obtained from Satureja cilicica P. H. Davis,
Satureja cuneifolia Ten, Satureja
hortensis L., Satureja spicigera (C.
Koch) Boiss., Satureja thymbra L. and
Satureja montana L. were tested against on the adults and larvae of
Colorado potato beetle (Leptinotarsa
decemlineata (Say, 1824)). The experiments were conducted in glass Petri
dishes and vacuum desiccators including 15 individual for each period with
three replicates under laboratory conditions. 10, 15 and 20 mg/mL doses of ethanol extracts conducted in the Petri dishes and the
desiccators showed that depending on concentration increase and duration of
exposure time resulted between 2.22-100% toxic effects on the potato beetle
larvae and adults. In Petri trials, the highest mortality rate was recorded as
100% for the second larval stage at the 20 mg/mL dose of S. spicigera ethanol extract after 96
hours the treatment. In desiccator experiments, the highest toxicity rate was
determined as 100% for first larval stage at the 20 mg/mL dose of S. thymbra ethanol
extract after 96 hours of the application. In addition to, when LD values of
the ethanol extracts ​​were taken into account the highest toxicity of adult
period was determined for S. thymbra extract (LD25:0.000, LD50:
0.010 μL/insect), the lowest toxicity was determined for S. cilicica extract (LD90:
436.020 μL/insect). The results obtained from this study suggested that the
ethanol extracts of tested Satureja L.
species could be used for L. decemlineata
larvae and adults as bio-larvicides and insecticides.

Keywords

Ethanol extracts, Leptinotarsa decemlineata, Satureja species, toxic effect

Supporting Institution

Ataturk University Scientific Research Projects

Project Number

BAP 2012/233

Thanks

This study is a part of master thesis supported by Ataturk University Scientific Research Projects (BAP 2012/233). The authors would like to thank Assoc. Prof. Dr. Memiş KESDEK (Mugla Sıtkı Kocman University) for valuable contributions and their helpful comments on the earlier versions of this manuscript.

Altı farklı Satureja L. türünden elde edilen etanol ekstraktının Patates Böceği, Leptinotarsa decemlineata (Say, 1824), (Coleoptera: Chrysomelidae) üzerindeki toksisitelerinin araştırılması

Abstract

Bu
çalışmada, Satureja cilicica P. H.
Davis, Satureja cuneifolia Ten, Satureja hortensis L., Satureja spicigera (C. Koch) Boiss., Satureja thymbra L. ve Satureja montana L. bitkilerinden elde
edilen ethanol ekstraktları patates böceğinin ergin ve larvaları üzerinde test
edilmiştir. Testler laboratuvar koşulları altında cam Petri ve vakumlu
desikatörlere yerleştirilmiş her bir döneme ait 15 bireyde 3 tekerrürlü olarak
yapılmıştır. Petri ve desikatör denemelerinde ethanol ekstraklarının 10, 15 ve
20 mg/mL’lik dozları konsantrasyon artışına ve maruz kalma süresine bağlı olarak
patates böceği larva dönemleri ve erginleri üzerinde 2.22-100% oranında toksik
etki göstermiştir. Petri denemelerinde, en yüksek ölüm oranı uygulanmadan 96 saat
sonra S. spicigera ethanol
ekstraktının 20 mg/mL’lik dozunda ikinci larva döneminde %100 olarak
kaydedilmiştir. Desikatör
denemelerinde ise, en yüksek toksisite oranı uygulamadan 96 saat sonra S. thymbra etanol ekstraktının 20 mg/mL’lik
dozunda birinci larva dönemi için % 100 olarak belirlenmiştir. Ek olarak,
etanol ekstraktlarının LD değerleri dikkate alındığında, en yüksek toksitite ergin dönemde S. thymbra ekstraktında
(LD25:0.000, LD50:
0.010 μL/böcek), en düşük toksitite ise S.
cilicica ekstraktında (LD90: 436.020 μL/böcek) olarak
belirlenmiştir. Bu
çalışmadan elde edilen sonuçlar, test edilen Satureja L. türleri etanol ekstraklarının L. decemlineata larvaları ve yetişkinleri için biyo-larvisit ve
insektisit olarak kullanılabileceğini göstermiştir.

Keywords

Etanol ekstraktı, Leptinotarsa decemlineata, Satureja türleri, toksik etki

Project Number

BAP 2012/233

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