anatol. j. bio.

Anatolian Journal of Biology

2687-444X

Mehmet KARADAYI

Structural Biology

Yapısal Biyoloji

Investigation of UV Protection of Pigments Obtained from DifferentBacteria on Wheat Plant

Gülmez

Özlem

ATATÜRK ÜNİVERSİTESİ

https://orcid.org/0000-0002-7452-9236

Tiryaki

Deniz

ATATURK UNIVERSITY

12 31 2021

2 2 21 24 12 24 2021 12 31 2021

2020

Anatolian Journal of Biology

In this study, UV protection of the same type of pigments obtained from different types of bacteria on wheat (Triticum aestivum cv Kirik) plant was investigated. The seedlings were grown in pots under optimal conditions for a total of 15 days. Pigments obtained from bacteria were applied on the 12th day, and after 24 hours, all UV was applied except for the control group. The plants harvested after the fifteenth day were used as experimental material. As a result of the application, the amounts of hydrogen peroxide (H2O2) and malondialdehyde (MDA) increased in the seedlings that were only UV applied. In the pigment applications we use as a preservative, it has been determined by the measurements that it reduces the UV damage. In addition, antioxidant enzyme activities; Superoxide dismutase (SOD), catalase (CAT) and peroxidase (POX) were examined and it was observed that these enzymes increased in pigment applications and showed protection against UV.

Bacterial pigments UV Plant resistance Triticum aestivum Bacterial pigments UV Plant resistance Triticum aestivum

[1] Narsing Rao., MP., Xiao, M., Li, W. J. Fungal and bacterial pigments: secondary metabolites with wide applications. Frontiers in microbiology, 2017. 8, 1113.

[2] Borić, M., Danevčič, T., & Stopar, D. Prodigiosin from Vibrio sp. DSM 14379; a new UV-protective pigment. Microbial ecology, 2011. 62(3), 528-536.

[3] Mohana D.C., Thippeswamy S., Abhishek R.U. Antioxidant, antibacterial, and ultraviolet-protective properties of carotenoids isolated from Micrococcus spp. Radiat Prot Environ, 2013. 36(4), 168.

[4] Poorniammal R., Balachandar D., Gunasekaran S. Evaluation of antioxidant property of some fungal pigments by DNA protection assay. AP, 2018, 7(1), 106-111.

[5] Venil C.K., Velmurugan P., Dufossé L., Renuka Devi P., Veera Ravi A. Fungal pigments: Potential coloring compounds for wide ranging applications in textile dyeing. J. Fungus, 2020. 6(2), 68.

[6] Pailliè-Jiménez M.E., Stincone P., Brandelli A. Natural pigments of microbial origin. Food syst, 2020. 4, 160.

[7] Pramono N.A., Sofyan F.I., Purwandani B.A., Ghaisyani O. Application of ESP32 as a Media for Learning Ozone Damage in the Form of IoT-Based Ultraviolet Index Readers. JODLI, 2020. 2(1), 22-29.

[8] Rai, A., & Agarwal, U. A.. Linking workplace bullying and work engagement: the mediating role of psychological contract violation. South Asian Journal of Human Resources Management, 2017. 4(1), 42-71.

[9] Coffey A., Prinsen E., Jansen M.A.K., Conway., J. The UVB photoreceptor UVR8 mediates accumulation of UV‐ absorbing pigments, but not changes in plant morphology, under outdoor conditions. Plant Cell Environ, 2017. 40(10), 2250-2260.

[10] Shatila F., Yusef H., Holail H. Pigment production by Exiguobacterium aurantiacum FH, a novel Lebanese strain. Int. J. Curr. Microbiol. App. Sci, 2013, 2(12), 176-191.

[11] Heath R.L, Packer L. Photo peroxidation in isolated chloroplast I. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 1968. 25:189–198.

[12] Patterson, B.D., MacRae, E. A., & Ferguson, I. B. Estimation of hydrogen peroxide in plant extracts using titanium (IV). Analytical biochemistry, 1984. 139(2), 487-492.

[13] Agarwal S, Pandey V. Antioxidant enzyme responses to NaCl stress in Cassia angustifoli. Biol Plant 2004. 48:555–560.

[14] Zhang J.X, Kirkham M.B. Drought stress-induced changes in activities of superoxide dismutase, catalase and peroxidase in wheat species. Plant Cell Physiol 1994. 35:785–79.

[15] Qiu Z.B., Li J.T., Zhang Y.J., Bi Z.Z., Wei H.F. Microwave pretreatment can enhance tolerance of wheat seedlings to CdCl2 stress. Ecotoxicol Environ Saf 2011. 74:820–825.

[16] Kasote, D. M., Katyare, S. S., Hegde, M. V., & Bae, H. Significance of antioxidant potential of plants and its relevance to therapeutic applications. International journal of biological sciences, 2015. 11(8), 982.

[17] Avalos, J., & Limón, M. C. Biological roles of fungal carotenoids. Current Genetics, 2015. 61(3), 309-324.

[18] Tiryaki D., Aydın, İ., & Atıcı, Ö. Psychrotolerant bacteria isolated from the leaf apoplast of cold-adapted wild plants improve the cold resistance of bean (Phaseolus vulgaris L.) under low temperature. Cryobiology, 2019. 86, 111-119.

[19] Gülmez Ö. Tiryaki D., Algue O.f., Koseoğlu M., Gezgincioglu E. Reduction of phytotoxic effect of cadmium heavy metal by biomass of edible fungus, Armillaria tabescens. Iranian Journal of Plant Physiology, 2020. 10(3), 3265-3272.

[20] Bulgari R., Franzoni, G., & Ferrante, ABiostimulants application in horticultural crops under abiotic stress conditions. Agronomy, . 2019. 9(6), 306.